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We present the results of evaluating the undifferentiated maintenance ability of FGF2 and the FGF2 alternative peptide "PG-011" using three types of human iPS cell lines. FGF2 (100 ng/mL, approximately 5.9 nM) or an equimolar concentration of PG-011 (approximately 30 ng/mL) was added to the culture medium, and after 7 days of culture, passaging was performed. Three passages were conducted, and the positive rate of undifferentiated markers in iPS cells on Day 7 of passage 3 (P3) was evaluated. As a result, the cell numbers at each passage were almost the same, confirming that "PG-011" exhibited similar proliferation ability to FGF2 in iPS cells. [Results] - iPS cells cultured with "PG-011" and FGF2 showed similar colony morphology. - The cell numbers at each passage were almost the same, confirming that "PG-011" exhibited similar proliferation ability to FGF2 in iPS cells. - The measurement results of undifferentiated markers in iPS cells on P3, Day 7 indicated that "PG-011" has an undifferentiated maintenance ability at the same level as FGF2. *For more detailed test results, please download the materials from the link below.

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We conducted experiments to promote the differentiation of iPS cells into hematopoietic stem progenitor cells (HSPC) using "PG-007," "PG-008," and "PG-010," which can be utilized in the differentiation induction process into NKT cells. We compared the productivity, differentiation induction efficiency of HSPC, as well as the productivity, differentiation induction efficiency, and functionality of NKT cells, with control conditions using existing methods such as VEGF, CHIR99021, and TPO. As a result, the differentiation induction process was shortened by one day, and more than twice the number of HSPC cells was obtained compared to existing methods. [Summary of Results] ■ "PG-007" demonstrated sufficient effect at one-tenth the concentration of VEGF, "PG-008" at one 2500th the concentration of CHIR99021, and "PG-010" at one-fifth the concentration of TPO. ■ The differentiation efficiency into NKT cells and the functionality of the obtained NKT cells were equivalent to the control conditions, while the number of NKT cells obtained significantly increased. *For more detailed test results, please download the materials from the link below.

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In the process of inducing differentiation of lung progenitor cells from human iPS cells, Noggin was used in the anterior foregut induction step. As an alternative to Noggin, "PG-004" was used, and the differentiation induction efficiency of anterior foregut cells and subsequently induced ventral anterior foregut cells and lung progenitor cells was compared. After maintaining and proliferating human iPS cells (HILC01 strain) in commercially available medium (mTeSR Plus-cGMP) in an undifferentiated state, a stepwise differentiation induction was performed using the collected undifferentiated iPS cells. As a result, "PG-004" showed a concentration-dependent increase in the induction efficiency of each cell type, and particularly, results equivalent to Noggin were obtained in the induction efficiency of ventral anterior foregut and lung progenitor cells. [Summary] ■ Stepwise differentiation induction of endodermal cells, anterior foregut cells, ventral anterior foregut cells, and lung progenitor cells. ■ Formation of alveolar organoids was also confirmed using lung progenitor cells created through the differentiation induction process with "PG-004." *For more detailed test results, please download the materials from the link below.

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Our company develops, manufactures, and sells functional peptides that can serve as alternatives to growth factors and cytokines, which are essential components of media used for cell proliferation and differentiation induction. Through innovative products that maximize the unique properties of peptides, we aim to solve the challenges faced by industries and contribute to the promotion and dissemination of regenerative medicine and cell therapy. If you have any concerns regarding growth factors or cytokines, please feel free to reach out to us. 【Business Description】 ■ Development, manufacturing, and sales of functional peptides that can serve as alternatives to growth factors and cytokines *For more details, please download the PDF or feel free to contact us.

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