Contracted analysis of intestinal bacteria, fungi, and yeast microbiota "amplicon sequencing analysis"
We accept a wide range of biological samples for analysis, including feces from humans and various animals, skin, and oral cavity, providing services from DNA extraction to data analysis.
● Contracted microbiome analysis of various samples such as intestinal microbiota, skin microbiota, and oral microbiota ● Amplicon sequencing analysis using next-generation sequencers (MiSeq, Illumina) ● Comprehensive support for DNA extraction, PCR, sequencing, and analysis in-house ● Options available for α and β diversity analysis, principal component analysis, cluster analysis, etc. ● Delivered data can also be used for academic papers ● Specimens can be stored at room temperature using our fecal collection kit Overview: Amplicon sequencing analysis is an excellent method for analyzing biological communities in samples containing diverse species. PCR amplification and sequencing analysis are performed using primers with barcode sequences added to the mixed DNA extracted from the samples to identify them, yielding tens of thousands to hundreds of thousands of sequences at once. A large amount of nucleotide sequences can be obtained in a single analysis, and comprehensive detection of bacteria (including fungi and yeasts), including minor bacteria, is possible. 【For more details, please check the related links】
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basic information
■ Acceptable Samples Category A: Feces, intestinal contents, rumen fluid Category B: Saliva, plaque, skin swabs, other than Category A ■ Specifications ● Set 1: - Analysis Software: Metagenome@KIN - Database: RDP, Technosulga Lab "Microbial Identification Database" ● Set 2: - Analysis Software: QIIME2 - Database: Greengenes, SILVA, UNITE ■ Delivery Contents ● Set 1 - NGS sequencing raw data (fastq files) - Sequence data after quality filtering and chimera check (fasta files) - Data analysis results (csv, html files) ● Set 2 - NGS sequencing raw data (fastq files) - QIIME2 output data files after quality filtering and chimera check (qzv files) → List of representative sequences and read counts, homology search results (tables, bar charts) ■ Sample Submission Method ● Please send frozen or refrigerated ● For feces, freezing is recommended ● Using our collection kit allows for refrigerated transport while preventing changes in the microbiome
Price range
P2
Delivery Time
※Depending on the selected course.
Applications/Examples of results
- Evaluation of functional foods through analysis of the gut microbiota (gut flora). - Confirmation of changes in the presence ratio of lactic acid bacteria and bifidobacteria based on the functionality of foods. - Academic research on the relationship between diseases and the gut microbiota (gut flora). - Analysis of the oral microbiota (oral flora). - Analysis of skin resident microbiota (skin flora) for product development. - Anti-aging research.
Company information
We offer contract analysis services related to microorganisms. We support our customers' testing, analysis, and research with a wide range of services, including microbial identification, microbial community structure analysis, and intestinal environment analysis. We meet diverse needs in academic research, product research and development, and hygiene management.