Detection of indole production by intestinal bacteria and certain anaerobic bacteria!
The "Spot Indole Reagent" is a product that demonstrates the ability of specific bacteria to decompose the amino acid tryptophan and convert it into indole, accumulating it in the medium. In the spot test, indole binds to p-dimethylaminocinnamaldehyde (DMACA) in an acidic pH within the filter paper matrix, producing a blue to blue-green compound. In a positive result, a color change from blue to blue-green appears in the bacterial smear sample within 2 to 3 minutes. If there is no reaction, it is considered negative. 【Specifications】 ■ Product Number: SG-SPOTIND-5 ■ Volume: 5mL *For more details, please download the PDF or feel free to contact us.
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【Instructions】 1. Use a sterile swab to collect a portion of the colony cultured for 18 to 24 hours from non-selective media. 2. Drop spot indole onto the collected sterile swab. *For more details, please download the PDF or feel free to contact us.
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As we enter the 21st century, the development of science and technology, including information technology (IT) and biotechnology, is accelerating, presenting new possibilities for people's lives. On the other hand, issues such as environmental pollution caused by chemical substances and the emergence and resurgence of infectious diseases due to microorganisms have not been overcome even with the latest scientific technologies, and are instead becoming more serious and complex. In this situation, the importance of the work of researchers, engineers, and healthcare professionals tackling these problems that threaten people's health will continue to grow. We aim to contribute to these individuals through the provision of instruments and consumables in the fields of environmental analysis, measurement, and microbiological research. We will reflect on what we can do and what we can uniquely offer, striving to provide unique value.
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