Simple screening reagent "TTC solution"

Established a simple and rapid screening method (TTC method) for E. faecalis and E. faecium!

The "TTC solution" is a simple screening reagent that establishes a rapid screening method (TTC method) for E. faecalis and E. faecium using the reduction ability of 2,3,5-triphenyltetrazolium chloride (TTC). TTC is originally white, but in physiologically active tissues, it is reduced by dehydrogenase enzymes to form an insoluble red compound called TPF (1,3,5-triphenylformazan). However, no color change occurs in tissues that lack activity. Various enzymes are involved in the reduction of TTC to TPF, including succinate dehydrogenase and flavin enzymes from the electron transport chain. 【Usage Instructions】 1. Collect one colony of bacteria grown on sheep blood agar at 37°C for 24 hours using a sterile swab. 2. Add 1-2 drops of TTC solution to the sterile swab that collected the colony. 3. Let it sit at room temperature for 2 minutes. *For more details, please download the PDF or feel free to contact us.*

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