Cell-friendly gene introduction: Next-generation transfection platform
Using the latest photoporation technology, a wide variety of cells, including iPS cells and CAR-T cells, can be introduced with various substances such as nucleic acids, proteins, and CRISPR/Cas9 RNPs. This method provides minimal damage during introduction, high survival rates, and achieves efficient transfection with high throughput. Photoporation is a gentle method for delivering payloads to cells based on the interaction between pulsed laser light and photothermal nanosenstizers. When laser light is applied to unique nanosenstizers adjacent to the cells, temporary pores are formed in the cell membrane through localized plasmonic reactions, allowing external payloads to enter the cytoplasm with minimal impact on cell survival and function. This technology serves as a next-generation transfection platform that efficiently, flexibly, and safely delivers various payloads to a wide range of cells, including primary cells, where transfection is challenging.
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Operating temperature: 25 °C Operating humidity range: up to 80% (without condensation) Storage temperature range: 15 to 35 °C Storage humidity range: up to 95%, relative humidity (without condensation) Available ports: USB 2.0 x 1 Ethernet RJ45 x 1 Screen size: 10.1-inch LCD display Screen resolution: 1280 x 800px (WXGA) Well plate: 6-well to 96-well plate, IBIDI chamber slide T flask: T-75 flask Pollution degree (IEC 60664-1 standard): 2* Power supply: 100 to 240 VAC 50/60 Hz Maximum current: 90V / 2.65A Maximum power consumption: ±265 W Dimensions: W500 x D450 x H350 mm Weight: 23.5 kg *Pollution degree 2: Equipment can be used in environments where only non-conductive pollution, similar to that of household appliances and office equipment, occurs.
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Applications/Examples of results
■iPS cell transfection using photoporation Efficient and gentle non-viral engineering ■Efficient delivery of macromolecules to stem cells derived from horses and humans ■Research on inflammasome activation using the photoporation method
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Our researcher customers are dedicated to their research day and night to "change the world" and "create the future." The products we handle reach the hands of researchers, contributing to the advancement of science and technology in Japan. Our company plays a role in creating such an exciting future and has been supporting researchers in the niche fields of environment, materials, and biotechnology as a top partner for 50 years since our establishment.