High-precision analysis of the complex physicochemical properties of mRNA medicines.

High-precision evaluation of molecular weight, structure, and aggregation using light scattering detectors and LC-MS.

mRNA has been gaining attention as a modality in recent years, but evaluation methods have not yet been established, posing challenges in product development. SEC-MALS (Size Exclusion Chromatography with Multi-Angle Light Scattering detection) serves as a powerful tool for evaluating important biophysical characteristics. By utilizing SEC-MALS, it is possible to measure molecular weight, size, and three-dimensional structure with high precision, obtaining essential information for the development and optimization of mRNA therapeutics. Furthermore, when combined with LC-MS, it enables more comprehensive characterization and biophysical property analysis. The use of LC-MS for the separation and analysis of synthetic nucleic acids is effective, allowing for the simultaneous acquisition of a wide range of information, including purity, impurity content, and sequence confirmation. With the enhancement of dedicated analysis software, anyone can easily perform data analysis, regardless of their experience with LC-MS. By clicking [Submit], you are deemed to have consented to Waters' use and processing of the information provided on this site in accordance with Waters' Terms of Use and Privacy Notice. Terms of Use and Privacy Notice www.waters.com/legalandprivacy

Light Scattering PhotometerMass SpectrometerHigh Performance Liquid Chromatograph