High-precision analysis of the complex physicochemical properties of mRNA medicines.
High-precision evaluation of molecular weight, structure, and aggregation using light scattering detectors and LC-MS.
mRNA has been gaining attention as a modality in recent years, but evaluation methods have not yet been established, posing challenges in product development. SEC-MALS (Size Exclusion Chromatography with Multi-Angle Light Scattering detection) serves as a powerful tool for evaluating important biophysical characteristics. By utilizing SEC-MALS, it is possible to measure molecular weight, size, and three-dimensional structure with high precision, obtaining essential information for the development and optimization of mRNA therapeutics. Furthermore, when combined with LC-MS, it enables more comprehensive characterization and biophysical property analysis. The use of LC-MS for the separation and analysis of synthetic nucleic acids is effective, allowing for the simultaneous acquisition of a wide range of information, including purity, impurity content, and sequence confirmation. With the enhancement of dedicated analysis software, anyone can easily perform data analysis, regardless of their experience with LC-MS. By clicking [Submit], you are deemed to have consented to Waters' use and processing of the information provided on this site in accordance with Waters' Terms of Use and Privacy Notice. Terms of Use and Privacy Notice www.waters.com/legalandprivacy
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basic information
Related Products - Multi-Angle Light Scattering (MALS) Detector DAWN Series - Mass Spectrometer BioAccord LC-MS System - Mass Spectrometer Xevo G3 QTof - Low-Adsorption UPLC System ACQUITY Premier - Low-Adsorption LC Column MaxPeak Premier Column
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Applications/Examples of results
- Evaluation of size distribution - Analysis of absolute molecular weight, size, and three-dimensional structure - Characterization of aggregates and fragmentation - Radius of gyration (Rg) - Hydrodynamic radius (Rh) - Rg/Rh ratio (indicator of conformation) - UV absorbance coefficient (at 260 nm) - Purity of mRNA (A260/A280) - 5' capping efficiency - 3’ poly A distribution - Oligo mapping - Impurity analysis - LNP (constituent lipids, impurities in lipids) If you have any requests for application notes, demo analyses, etc., please feel free to contact us.
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Company information
Waters Corporation, headquartered in Milford, Massachusetts, USA, has played a pioneering role as a specialized manufacturer of liquid chromatography since its establishment in 1958. Actively engaged in the development of new products and technologies, it has grown into an international group company that supplies not only high-performance liquid chromatography but also mass spectrometers (MS) and thermal analysis instruments. Waters Japan started operations in 1973 (Showa 48) as the Japanese base of Waters Corporation. Inheriting the corporate ethos of the U.S. headquarters, we have made "Customer Success" our mission and continue to provide innovative technologies and lab solutions.