Detection of difficult-to-culture microorganisms suspected to be the cause of swelling in packaged foods through microbial community analysis.
Understanding microorganisms that could not be detected through cultivation. Providing a new perspective for cause investigation using non-cultivation methods.
In cases of product abnormalities such as swelling in packaged foods, gas production by microorganisms is suspected to be the cause. However, there are often instances where microbial growth cannot be confirmed even after conducting culture tests, making it difficult to determine the cause. In this case, a microbial confirmation test using microscopic observation was conducted, followed by an attempt to isolate microorganisms using culture methods, but no colony growth was observed. By employing amplicon sequencing analysis, a non-culture method, we were able to obtain information on microorganisms that cannot be captured through culture. Amplicon sequencing analysis allows for direct DNA analysis from samples, enabling a comprehensive understanding of the microbial composition (microbiota) present in the sample, independent of culture conditions. While the results obtained do not specifically identify the cause of the product abnormality, they can be utilized as foundational information to narrow down potential causes and advance discussions on countermeasures.
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basic information
- Target samples: Abnormal products such as packaged foods (swelling, spoilage, etc.) - Test contents: - Microbial confirmation test (microscopic observation) - Microbial isolation (aerobic and anaerobic conditions, media modification) → In this case, no colony growth was observed - Amplicon sequencing analysis (microbiome analysis) - Features of amplicon sequencing analysis (microbiome analysis): - Direct analysis of microbial-derived DNA without dependence on culture conditions - Detection of difficult-to-culture or dead microorganisms - Comprehensive understanding of the composition ratio of microorganisms (at the genus level)
Price information
- Price estimate (excluding tax): - Microbial confirmation: 12,000 yen/sample - Microbial isolation: 18,000 yen/sample (due to no colony growth observed) + 4,000 yen/sample (anaerobic culture fee) - Amplicon sequencing analysis: 19,000 yen/sample - Total: approximately 53,000 yen/sample
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Applications/Examples of results
- Investigation of the causes of swelling in packaged foods (gas generation), spoilage, and off-odors - Analysis of cases where microorganisms could not be detected using culture methods - Reevaluation of culture conditions based on microbiome information
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Company information
We offer contract analysis services related to microorganisms. We support our customers' testing, analysis, and research with a wide range of services, including microbial identification, microbial community structure analysis, and intestinal environment analysis. We meet diverse needs in academic research, product research and development, and hygiene management.
