State-of-the-art stop flow system
The stopped-flow method is a technique that rapidly mixes a sample solution and instantly stops the flow, allowing for the high-speed measurement of changes in the absorption spectra and fluorescence of the sample solution in the visible, ultraviolet, and near-infrared regions. It can measure the reaction rates of various fast reactions on the order of milliseconds to seconds, such as homogeneous catalyst reactions, enzyme reactions, redox reactions, structure formation, and particle generation, as well as detect short-lived intermediates. This enables the evaluation of reaction activity, derivation of activation energy, analysis of reaction mechanisms, assessment of inhibitors and promoters, and comparison of the properties of related substances and new materials.
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basic information
The stopped-flow method rapidly mixes samples and tracks the reaction process using time-resolved absorption spectra in the ultraviolet-visible-near-infrared range. - Spectra can be measured at intervals as short as 1 ms, with a maximum of 16,384 measurements. - Pseudo-logarithmic sampling allows for measurements from milliseconds to minutes to hours in a single shot. - The wavelength-time axis conversion function and nonlinear least squares fitting function enable the calculation of reaction rates on-site from the measured data. - It is also compatible with fluorescence measurements and flow flash measurements combined with lasers. - Measurements in the near-infrared region are also supported.
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Applications/Examples of results
【Examples of Use】 - Characterization of novel metal complexes/organic synthesis materials such as porphyrin-based and fullerene-based compounds - Analysis of reaction mechanisms of reactive oxygen species/free radicals/antioxidants - Measurement of drug properties - Analysis of nanoparticle synthesis processes - Analysis of reaction mechanisms and characterization of artificial heme proteins - Analysis of interactions between proteins and ligands - Analysis of the reaction processes of protein folding - Activity evaluation of membrane proteins such as aquaporins Additionally, we accommodate various options and custom requests.
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Company information
Unisoku was established in 1974 with the aim of becoming a creator of unique measuring instruments. It is a manufacturer that primarily develops and sells its own ultra-high vacuum scanning probe microscopes (SPM) to universities, public institutions, and private companies and research institutes. Over the years, it has developed and commercialized advanced measuring instruments such as high-speed spectroscopic measurement devices and scanning tunneling microscopes, delivering them to universities and research institutions both domestically and internationally, and its technological content has received high praise from researchers in the field of basic research. In recent years, scanning probe microscopes have significantly improved in performance and multifunctionality, particularly as a means of observing sample surfaces at the atomic level. Their applications are expanding mainly from the field of physics to chemistry and bio-related fields, making them indispensable tools in nanotechnology and nanoscience.