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HPLC Product List

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HPLC Method Transfer: Adjustment Function for Gradient Delay Volume

HPLC Method Transfer: Adjustment Function for Gradient Delay Volume

A detailed explanation of one of the most important parameters of method transfer, gradient delay volume.

Transferring methods from old HPLC equipment to new devices or between devices from different manufacturers is a challenging task for many HPLC users. Differences in hardware design, such as the temperature control mode of the column oven, pre-column heater, and specifications of the pump—critical for gradient methods—as well as the design of the auto-sampler and the system volume determined by the plumbing, can affect the results of chromatography. This technical note introduces the gradient delay volume, which is extremely important in method transfer.

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  • Analytical Equipment and Devices
  • HPLC

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Automate and remote the analysis workflow! New product Agilent HPLC.

Automate and remote the analysis workflow! New product Agilent HPLC.

Accelerating Automation and DX: The Arrival of Agilent Infinity III LC with Intelligence Features

The new Agilent Infinity III LC is a liquid chromatograph that automates and remote-controls analytical workflows, making your lab smarter. It streamlines routine and ancillary tasks, allowing you to focus on R&D and creative work, while reducing human error and stress. **Features** - **Automation and Remote Control** Streamlines daily tasks from mobile phase handling to sample management. You can monitor the status of the device from your office or home. - **Maximizing Uptime** Uses troubleshooting and maintenance guides to maximize device uptime. Even if the LC personnel change due to organizational restructuring or employee turnover, the system can be fully operational immediately. - **Investment Protection** Existing Infinity II LC systems can be easily upgraded with the latest Agilent Infinity III LC technology. *Please download the leaflet with a comparison table to conventional HPLC here (PDF).*

  • Analytical Equipment and Devices
  • HPLC

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HPLC & UHPLC Comprehensive Catalog 2023-2024 Edition

HPLC & UHPLC Comprehensive Catalog 2023-2024 Edition

Worldwide bestseller LC

Since Agilent launched the HP1010 LC system in 1973, it has consistently incorporated customer feedback and focused on improving system performance. During this time, there was a period when we had development and manufacturing bases in Germany and Japan (Yokogawa Electric Corporation, Yokogawa Analytical Systems), but now all LC systems are "Made in Germany." By integrating the latest technologies, we have been recognized as a worldwide bestseller in LC systems, addressing various needs such as pharmaceutical development, food safety assurance, chemical and material analysis, and fractionation, while also complying with global regulations. Currently, Agilent has formed a teamwork approach among LC instruments, columns, consumables, mass spectrometers, software, and service/support under the InfinityLab series, continuously evolving to maximize daily analysis, equipment, and lab efficiency.

  • Analytical Equipment and Devices
  • Contract measurement
  • HPLC

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Recycling Fractionation HPLC LaboACE

Recycling Fractionation HPLC LaboACE

This is a preparative HPLC aimed at the isolation and purification of target compounds.

The highly acclaimed LaboACE series has further evolved, with significantly improved recovery rates and purity. With a combination of comprehensive automatic functions and high-speed preparative GPC columns, you can rely on us for large-scale preparative needs! You can program up to 30 tasks (events) for a single chromatogram, including sample injection, cutting unnecessary peaks, recycling, and fractionation, and repeat the entire sequence up to 99 times. Additionally, the built-in fraction collector in the main unit can accommodate various container sizes, making it ideal for large-scale fractionation of the same sample. 【Features】 - Compact body equipped with comprehensive automatic functions - Standard recycling function that promotes separation by repeatedly passing through the column (no solvent is consumed during recycling). - Automatic cleaning function for automatic flow path cleaning - Easy operation with a large LCD touch panel - PC control function (optional) to operate the main unit via the included PC - Low-pressure two-liquid gradient capability is also available (optional) *Manual models are also available in the lineup. For more details, please feel free to contact us.

  • Separation device
  • HPLC

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Vanquish Core HPLC System for Routine Analysis

Vanquish Core HPLC System for Routine Analysis

A reliable HPLC system that supports stable routine analysis.

The Thermo Scientific Vanquish Core HPLC system is based on the innovative Vanquish platform, achieving reliable performance and durability through excellent analytical precision, detector sensitivity, and operability. Designed for routine analysis laboratories, it features: - Regular automatic system health checks and improved robustness to provide reliable data throughout uptime. - Simple operability through the Thermo Scientific Chromeleon Chromatography Data System (CDS), maximizing performance while ensuring data integrity and preventing data tampering and falsification. - System diagnostics that can identify equipment issues and a solvent monitoring system that measures mobile phase consumption and waste volume in real-time, minimizing unexpected or non-compliant results. - Intuitive operability and simplified maintenance supported by videos, enhancing productivity regardless of the operator's skill level. - Easy method transfer from various HPLC systems without hardware configuration changes, making it suitable as a backup system.

  • Analytical Equipment and Devices
  • HPLC

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[Case Study] Method Transfer for HPLC Purification and Analysis of Oligonucleotides

[Case Study] Method Transfer for HPLC Purification and Analysis of Oligonucleotides

Streamlining the process from high-purity fractionation of double-labeled oligos to quality confirmation using HRAM-MS.

This application note presents a case study of transferring the purification and analysis workflow of synthetic oligonucleotides from a competitor's HPLC system to the Thermo Scientific Vanquish HPLC system. Focusing on dual-labeled oligonucleotide samples, the workflow was validated through purification via semi-preparative RP-HPLC, purity assessment using HPLC-UV, and identity confirmation with HRAM-MS. A comparison using the Hypersil GOLD column demonstrated the ability to isolate the target oligonucleotides with high purity, achieving efficient quality control. The note provides practical comparative data on retention behavior, peak shape, and collection capacity differences during method transfer. **Features** - Case study on the transfer from a competitor's LC purification system to the Vanquish analytical purification LC system. - Validation of purification, purity assessment, and identity confirmation of dual-labeled oligonucleotides as a complete workflow. - Comparison of separation performance between the Hypersil GOLD column and competitor columns. - Explanation of differences in peak shape and collection capacity with specific chromatograms. - Introduction of an efficient quality control method combining HPLC-UV and HRAM-MS.

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  • Analytical Equipment and Devices
  • HPLC

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[Case Study] Efficient Analysis of Sunscreen Ingredients Using UHPLC System

[Case Study] Efficient Analysis of Sunscreen Ingredients Using UHPLC System

Rapid and accurate analysis of multiple ultraviolet filter components using a high-precision, environmentally friendly UHPLC method!

This technical document introduces a method for efficiently analyzing ten types of ultraviolet filter components contained in five types of sunscreen using a UHPLC system under a single analytical condition. The results of the validation of the method's accuracy and precision showed that the peak area RSD was below 1.10%, and the retention time RSD was below 0.50%, indicating high precision. Additionally, by transitioning the existing analytical method to the UHPLC method, we reduced solvent consumption and waste generation, and replaced tetrahydrofuran (THF) used in the sample solution with a more environmentally friendly solvent, thereby reducing the environmental impact. 【Features】 ■ Efficient analysis of ten types of ultraviolet filter components under a single analytical condition ■ High precision with peak area RSD below 1.10% and retention time RSD below 0.50% ■ Reduction of solvent consumption and waste generation through the UHPLC method ■ Thorough evaluation of accuracy, linearity, and precision ■ Use of environmentally friendly solvents to reduce environmental impact *For more details, please download the PDF or feel free to contact us.

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  • Other measurement, recording and measuring instruments
  • HPLC

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[HPLC Case Study] Automatic Derivatization of Amino Acids and Quantification of D-Amino Acids

[HPLC Case Study] Automatic Derivatization of Amino Acids and Quantification of D-Amino Acids

High-precision and high-sensitivity analysis of D/L amino acids using online derivatization with an autosampler.

This application note introduces amino acid analysis utilizing the automatic derivatization feature of the Thermo Scientific Vanquish Flex UHPLC system. Online chiral derivatization using OPA/NAC was employed to conduct quantitative analysis of D-aspartic acid (D-Asp), D-glutamic acid (D-Glu), and D-serine (D-Ser). By automating the process from derivatization to injection within the auto-sampler, we streamline complex sample preparation while achieving high reproducibility and quantitativeness. Furthermore, we demonstrate the quantification of D-amino acids present in fermented foods such as apple cider vinegar, grain vinegar, and whey, showing that sensitive and selective analysis is possible even in complex sample matrices. **Features** - Separation of D and L forms of eight amino acids including D-Asp, D-Glu, and D-Ser in under 15 minutes. - High reproducibility confirmed with retention time RSD ≦ 0.16% and area value RSD ≦ 0.22%. - Good linearity in the calibration range of 1–500 ng/mL (R² ≧ 0.9999). - Examples of D-amino acid quantification in fermented foods (apple cider vinegar, grain vinegar, whey) along with chromatograms are presented.

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  • Analytical Equipment and Devices
  • HPLC

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