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HPLC/UHPLC column "FlexFire C18"

HPLC/UHPLC column "FlexFire C18"

Making HPLC⇔UHPLC more accessible! This is a column that uses refined silica gel.

The "FlexFire C18" is a highly versatile HPLC/UHPLC column that emphasizes hydrophobic interactions due to high carbon content. It provides results that are faithful to the fundamentals of HPLC (UHPLC) and offers important information to establish future directions. It allows for easy setting of analytical conditions, as it can be transferred between quality control and research & development according to the system. 【Features】 ■ Extremely versatile ■ Emphasizes hydrophobic interactions due to high carbon content ■ Available in 5μm, 2.6μm, and 1.6μm ■ Transferable between quality control and research & development according to the system ■ Utilizes refined silica gel *For more details, please refer to the PDF document or feel free to contact us.

  • Other protein analysis
  • Column Chromatography

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HPLC/UHPLC column "FlexFire AQ C18"

HPLC/UHPLC column "FlexFire AQ C18"

Retention and separation of highly polar compounds in reverse phase systems! It can be handled just like before.

The "FlexFire AQ C18" is a column that can perform well across a wide range in both first and second applications. In reversed-phase columns, retaining and separating highly polar compounds can be extremely challenging. As a result, it is often the case that HILIC columns are chosen, but this column has been developed as an additional option before transitioning to HILIC columns. The frequently used C18 group in familiar reversed-phase columns has no change in usage, allowing for handling in the same manner as before. 【Features】 ■ Suitable for retaining and separating highly polar compounds in reversed-phase systems ■ Can perform well across a wide range in both first and second applications ■ Serves as an additional option before transitioning to HILIC columns ■ Can be handled in the same manner as before ■ Utilizes refined silica gel *For more details, please refer to the PDF document or feel free to contact us.

  • Other protein analysis
  • Column Chromatography

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Column "FlexFire C8"

Column "FlexFire C8"

For the separation of methods and improvement of peak shapes! We provide retention and separation, not time savings.

The "FlexFire C8" is a column developed primarily based on hydrophobic interactions, just like the FlexFire C18. Traditionally, the short alkyl chain provided advantages in time reduction, but it offers retention and separation instead of time savings. It is useful for improving the separation and peak shape of established methods. 【Features】 ■ Developed primarily based on hydrophobic interactions, like the FlexFire C18 ■ Provides retention and separation instead of time savings ■ Useful for improving the separation and peak shape of established methods ■ Despite having a carbon content of 12%, its bonding density is almost the same as that of C18 ■ Utilizes refined silica gel *For more details, please refer to the PDF document or feel free to contact us.

  • Other protein analysis
  • Column Chromatography

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HPLC/UHPLC column "FlexFire C30"

HPLC/UHPLC column "FlexFire C30"

Dramatically updated! Not just a second choice, but also active as a main column.

The "FlexFire C30" was introduced in 1998 and has been a representative column used by many people to this day. Updates include an expanded pH range, improved pressure resistance, and enhanced retention and separation of highly polar compounds. Analysis with a 100% aqueous mobile phase can still be set up as before. It serves not just as a second choice, but also as a main column. 【Features】 ■ Expanded pH range ■ Improved pressure resistance ■ Enhanced retention and separation of highly polar compounds ■ Serves as a main column, not just a second choice ■ Utilizes refined silica gel *For more details, please refer to the PDF materials or feel free to contact us.

  • Other protein analysis
  • Column Chromatography

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HPLC/UHPLC column "FlexFire mAb-RP"

HPLC/UHPLC column "FlexFire mAb-RP"

From intact protein to post-digestion peptide analysis! This single column completes it all.

"FlexFire mAb-RP" is a column developed for HPLC/UHPLC using 1000Å silica gel as the substrate. This single column can complete the analysis of macromolecules such as intact proteins and monoclonal antibodies, as well as digested peptides. By matching the molecular weight of the compounds with the pore size of the silica gel substrate, it enables more sensitive analysis. [Features] - Developed using 1000Å silica gel as the substrate - Supports analysis from intact proteins to digested peptides - Enables more sensitive analysis by matching the molecular weight of the compounds with the pore size of the silica gel substrate *For more details, please refer to the PDF document or feel free to contact us.

  • Other protein analysis
  • Column Chromatography

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FlexFire SEC Series Columns for HPLC/UHPLC

FlexFire SEC Series Columns for HPLC/UHPLC

Easily analyze polymer compounds! Methods can also be easily constructed.

The "FlexFire SEC Series" is a column designed for the separation of macromolecules such as proteins and antibodies, as well as low molecular weight compounds, based on their size in solution. This product primarily utilizes isocratic elution, and methods can be easily constructed. Additionally, the results obtained are straightforward, making it suitable for the analysis of intact biomacromolecules such as proteins and antibodies. 【Features】 ■ Easy analysis of macromolecules ■ Separation based on size ■ Primarily uses isocratic elution, with easy method construction ■ Simple results obtained ■ Well-suited for the analysis of intact biomacromolecules such as proteins and antibodies *For more details, please refer to the PDF document or feel free to contact us.

  • Other protein analysis
  • Column Chromatography

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Column "FlexFire HILIC"

Column "FlexFire HILIC"

Building methods with organic solvent composition rich! Improved detection sensitivity in LC/MS is expected.

"FlexFire HILIC" simply utilizes silica gel as the base material to achieve the retention and separation of highly polar compounds. By adding a 1.6μm particle size, which was not previously available in the lineup, it enables time savings and solvent reduction through ultra-fast analysis. Additionally, analysis in normal phase mode using non-aqueous solvents is possible, allowing for behaviors not seen in reverse phase columns. [Features] ■ Improved retention and separation of highly polar compounds ■ Expected enhancement in detection sensitivity in LC/MS ■ Time savings and solvent reduction through ultra-fast analysis ■ Analysis in normal phase mode using non-aqueous solvents is also possible ■ Utilizes refined silica gel *For more details, please refer to the PDF document or feel free to contact us.

  • Other protein analysis
  • Column Chromatography

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FlexFire Metal-Free Column

FlexFire Metal-Free Column

Introduction to LC/MS is also easy! There are analyses that can be made possible with hardware changes!

The "FlexFire Metal-Free Column" is an HPLC/UHPLC column that has a significant effect on compounds such as phosphate groups and metal coordination. Traditionally, these compounds have been analyzed using stainless steel columns with phosphate buffer methods. By using this product, it is possible to develop methods using formate buffers, making it easier to introduce into LC/MS. 【Features】 - Prevents adsorption by avoiding contact with metals - Allows for direct elution of compounds - Has a significant effect on compounds such as phosphate groups and metal coordination - Enables method development using formate buffers *For more details, please refer to the PDF document or feel free to contact us.

  • Other protein analysis
  • Column Chromatography

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HPLC/UHPLC column "FlexFire WP C4"

HPLC/UHPLC column "FlexFire WP C4"

For the analysis of macromolecules such as proteins and antibodies! It greatly contributes to the analysis of biopharmaceuticals.

The "FlexFire WP C4" is a column designed to target macromolecules such as proteins and antibodies. To accommodate various analytical conditions, its pH and temperature durability have been significantly upgraded compared to previous models, greatly contributing to the analysis of biopharmaceuticals in the future. Additionally, it can also be applied to the analysis of intact mAbs, in addition to proteins. 【Features】 ■Elutes proteins with various properties based on hydrophobic differences, primarily aimed at analyzing primary structural characteristics. ■Applicable for the analysis of intact mAbs. ■Ensures durability at 80°C, allowing for sharp peak elution. *For more details, please refer to the PDF document or feel free to contact us.

  • Other protein analysis
  • Column Chromatography

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HPLC/UHPLC column "FlexFire C1"

HPLC/UHPLC column "FlexFire C1"

It is possible to shorten analysis time! It has surprising performance that enhances the retention of highly polar compounds to the maximum limit.

The first choice of columns in HPLC/UHPLC method development is almost always C18, and to change selectivity from C18, columns such as C30, C8, and HILIC are selected as second choices. The 'FlexFire C1' is a column that uses refined silica gel. The perception of "this product = low adsorption" holds unexpected performance that enhances peak shape and peak sensitivity, as well as maximizes the retention of highly polar compounds. 【Features】 ■ Contains unexpected performance that maximizes the retention of highly polar compounds ■ Significantly weakens excessively strong retention ■ Allows for reduced analysis time without changing the method ■ Utilizes refined silica gel *For more details, please refer to the PDF document or feel free to contact us.

  • Other protein analysis
  • Column Chromatography

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