We have compiled a list of manufacturers, distributors, product information, reference prices, and rankings for HPLC Columns.
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HPLC Columns Product List and Ranking from 16 Manufacturers, Suppliers and Companies

Last Updated： Aggregation Period:Sep 17, 2025～Oct 14, 2025
This ranking is based on the number of page views on our site.

HPLC Columns Manufacturer, Suppliers and Company Rankings

Last Updated： Aggregation Period:Sep 17, 2025～Oct 14, 2025
This ranking is based on the number of page views on our site.

  1. 関東化学株式会社 バイオケミカル部 Tokyo//Chemical
  2. 東ソー バイオサイエンス事業部　計測分野 Tokyo//Chemical
  3. サーモフィッシャーサイエンティフィック株式会社／Thermo Fisher Scientific K.K. Tokyo//Testing, Analysis and Measurement
  4. 4 日本ウォーターズ（米国本社 Waters Corporation）　 Tokyo//Testing, Analysis and Measurement
  5. 5 ADSTEC Corporation Bio Department Chiba//others
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HPLC Columns Product ranking

Last Updated： Aggregation Period:Sep 17, 2025～Oct 14, 2025
This ranking is based on the number of page views on our site.

  1. High Purity Silica Gel HPLC Column "Mightysil Series" 関東化学株式会社 バイオケミカル部
  2. High-performance columns for HPLC, UHPLC, and UPLC [CORTECS] 日本ウォーターズ（米国本社 Waters Corporation）　
  3. HPLC column for sugar analysis - "CarboSep Series" ADSTEC Corporation Bio Department
  4. DNAPac PA200/PA200 RS Column サーモフィッシャーサイエンティフィック株式会社／Thermo Fisher Scientific K.K.
  5. 5 HPLC Column "Reversed Phase / HILIC" 東ソー バイオサイエンス事業部　計測分野

HPLC Columns Product List

31～45 item / All 79 items

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Oligo analysis HPLC column OligoSep Cartridge

Oligo analysis HPLC column OligoSep Cartridge

High-performance liquid chromatography (HPLC) column for oligonucleotide analysis

The OligoSep Cartridge manufactured by ADS Biotec (formerly Transgenomic) is a column for oligonucleotide analysis using ion-pair reverse phase HPLC. The packing material consists of non-porous beads made of alkylated polystyrene-divinylbenzene. Due to the non-porous nature of the beads, it allows for higher precision purification. It can be used not only for quality control of oligonucleotides but also for high-precision analysis of both single-stranded and double-stranded DNA. It is compatible with various types of high-performance liquid chromatography (HPLC) systems. By using buffers manufactured by ADS Biotec as the mobile phase, high-precision separation can be achieved. Additionally, by following the procedures outlined in the instruction manual to wash the column with 75% acetonitrile, the column can be reused multiple times.

  • High Performance Liquid Chromatograph

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HPLC column for oligonucleotide HPLC purification - OligoSep Prep HC Cartridge

HPLC column for oligonucleotide HPLC purification - OligoSep Prep HC Cartridge

High-performance liquid chromatography (HPLC) column for the purification of synthetic oligonucleotides.

The OligoSep Prep HC Cartridge manufactured by ADS Biotec (formerly Transgenomic) is a column for the purification of oligonucleotides using ion-pair reverse phase HPLC. It uses porous beads made of alkylated polystyrene-divinylbenzene (PS-DVB). Due to the porous nature of the beads, the surface area is increased, allowing for the purification of larger amounts of samples. It can be used for the purification of synthetic oligos. It is compatible with various types of high-performance liquid chromatography (HPLC) systems. By using ADS Biotec's buffer as the mobile phase, high-precision separation can be achieved. Additionally, by following the procedures outlined in the instruction manual and washing the column with 75% acetonitrile, the column can be reused multiple times.

  • High Performance Liquid Chromatograph

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Application Note: HPLC Products for Oligonucleotide Analysis

Application Note: HPLC Products for Oligonucleotide Analysis

HPLC column and buffer for oligonucleotide analysis

The HPLC columns and buffers for oligonucleotide analysis manufactured by ADS Biotec in the United States use beads made of alkylated polystyrene-divinylbenzene. The columns are referred to as the OligoSep series. Oligonucleotides are used in research for antisense and siRNA pharmaceuticals.

  • High Performance Liquid Chromatograph

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mRNA analysis HPLC products RNASep series

mRNA analysis HPLC products RNASep series

Capable of handling everything from mRNA analysis to purification! RNASep series

The RNASep series from ADS Biotec in the United States is capable of handling everything from mRNA analysis to purification.

  • High Performance Liquid Chromatograph

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HPLC column for optical isomer separation

HPLC column for optical isomer separation

HPLC column for optical isomer separation

Optical isomers present in various bioactive substances (such as pharmaceuticals, pesticides, and fragrances) that are deeply related to our daily lives are known to exhibit significantly different biological activities between isomers, increasing the necessity for the analysis and separation of optical isomers. The "SUMICHIRAL OA series" HPLC columns for the separation of optical isomers are gaining attention in fields such as pharmaceuticals and pesticides.

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F5 (PFP) HPLC/UHPLC column

F5 (PFP) HPLC/UHPLC column

Separation of positional isomers using reverse phase and HILIC analysis! An additional step to add to C18 and HILIC columns.

The "F5 (PFP) HPLC/UHPLC column" for metabolomics is a reversed-phase column with selectivity different from C18. By incorporating it as a second or third choice column in reversed-phase systems, the range of substances for LC analysis is broadened. Four retention mechanisms—hydrogen bonding, dipole-dipole interactions, π-π interactions, and hydrophobic interactions—complexly influence each other to exhibit unique retention characteristics and stereoselectivity. 【Features】 ■ Retains highly polar basic compounds without ion-pair reagents ■ 100% water mobile phase is usable ■ Suitable for LC/MS due to retention at high organic solvent concentrations ■ Supports both analysis and fractionation *For more details, please refer to the PDF document or feel free to contact us.

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Ascentis Express HILIC Guide

Ascentis Express HILIC Guide

Retention and separation of hydrophilic compounds made faster! Choose the right column for your needs with the HILIC stationary phase selection guide.

This document provides an explanation of HILIC and features various types of HILIC stationary phase columns. HILIC technology, which has complementary properties to both reverse phase (RP) and normal phase (NP) chromatography, is often used in studies analyzing hydrophilic analytes. Additionally, we offer a lineup of products including the "Ascentis Express OH5 HPLC column," "Ascentis Express ES-Cyano HPLC column," and others. We invite you to read through it. [Contents (excerpt)] ■ Ascentis Express U/HPLC columns ■ What is HILIC and reasons to choose it ■ Development of HILIC methods ■ Ascentis Express OH5 HPLC column *For more details, please refer to the PDF document or feel free to contact us.

  • Analytical Equipment and Devices

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[Data] RP-Amide HPLC/UHPLC Column

[Data] RP-Amide HPLC/UHPLC Column

Improvement of retention for carboxylic acids and phenolic compounds! Achieving low bleed suitable for LC-MS applications.

This document provides an explanation of "RP-Amide HPLC/UHPLC columns." It introduces the "characteristics of RP-Amide," the "differences in retention for neutral analytes," and the "increased retention for hydrogen bond donors." Additionally, it includes analyses of "ulcer medications using Ascentis RP-Amide" and "UHPLC measurements of sunscreen drugs using Ascentis Express RP-Amide, 2.7μm." We encourage you to read it. 【Contents】 ■Ascentis RP-Amide HPLC column ■Analysis of ulcer medications using Ascentis RP-Amide ■UHPLC measurements of sunscreen drugs using Ascentis Express RP-Amide, 2.7μm ■Data sheet on the care and use of Ascentis Express RP-Amide columns ■LC/MS workflow products ■Ascentis RP-Amide product list ■Fused-Core product list *For more details, please refer to the PDF document or feel free to contact us.

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Acclaim Surfactant Plus Column

Acclaim Surfactant Plus Column

For the analysis of consumables such as detergents, pharmaceuticals, food and beverages, and environmental samples! HPLC column.

The "Acclaim Surfactant Plus Column" is a state-of-the-art HPLC column with high efficiency and silica-based material. It allows for the simultaneous analysis of cationic surfactants, anionic surfactants, nonionic surfactants, and amphoteric surfactants. It can be used for the analysis of consumables such as detergents, pharmaceuticals, food and beverages, and environmental samples. 【Features】 ■ Selectivity suitable for the simultaneous analysis of cationic surfactants, anionic surfactants, nonionic surfactants, and amphoteric surfactants ■ Measurement of cationic surfactants ■ Separation of ethoxylated surfactants ■ Retention of highly hydrophilic compounds such as hydrotropes *Please feel free to contact us for more details.

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HPLC column "Acclaim Trinity P2 column"

HPLC column "Acclaim Trinity P2 column"

An ideal solution for counterion analysis in pharmaceuticals.

The "Acclaim Trinity P2 Column" is a high-performance silica-based column. It is particularly suitable for HPLC analysis of counter ions in charged pharmaceuticals. The usage is simple, it boasts excellent robustness, and it allows for the analysis of both monovalent and multivalent ions using fast general-purpose methods. Using a general-purpose HPLC, it is possible to effectively analyze counter ions with just one column and one type of analytical method. 【Features】 ■ Effectively analyzes ions using HPLC ■ Enables screening of counter ions present in pharmaceuticals ■ Retains ions or ionic compounds without using ion-pair reagents ■ Simple to use ■ Provides reproducible packing *For more details, please refer to the PDF materials or feel free to contact us.

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DNAPac PA200/PA200 RS Column

DNAPac PA200/PA200 RS Column

High separation of oligonucleotide chains and stereoisomers! Anion exchange column.

The "DNAPac PA200 HPLC/DNAPac PA200 RS (High-Speed Separation) UHPLC Column" is a column designed for the high separation of ssDNA and RNA. The selectivity for oligonucleotides can be controlled by various parameters such as the pH of the mobile phase, solvents, and the use of different salts, allowing for adjustments in separation to meet the specific requirements of applications. We provide structural analysis and quantification methods utilizing high-resolution mass spectrometry and anion exchange columns for UHPLC. 【Features】 ■ High separation of oligonucleotide chains and stereoisomers ■ Improved separation and throughput ■ Control of selectivity and retention through the pH of the mobile phase, type of salt, and type of mobile phase ■ Separation of n from n-1 bases *For more details, please refer to the PDF document or feel free to contact us.

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Basics of Ion Chromatography: Anion Exchange Column

Basics of Ion Chromatography: Anion Exchange Column

A clear explanation of the basics of ion chromatography! Let's understand the anion column, which is important in analysis.

If optimization of separation cannot be achieved by adjusting the elution solution or temperature, it is recommended to change the type of column. Using columns with different performance can significantly alter the separation depending on the ionic components. We will explain our anion columns.

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Basics of Ion Chromatography: Cation Exchange Column

Basics of Ion Chromatography: Cation Exchange Column

A clear explanation of the basics of ion chromatography! Let's understand the important cation columns in analysis.

If optimization of separation cannot be achieved by adjusting the elution liquid or temperature, it is recommended to change the type of column. Using columns with different performance can significantly alter the separation depending on the ionic components. We will explain our cation columns.

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Acclaim Mixed-Mode HPLC Column

Acclaim Mixed-Mode HPLC Column

A column that is expected to provide high separation.

Liquid chromatography aims to achieve good separation ability to obtain meaningful information through peak resolution. In mixed-mode chromatography, numerous parameters can be adjusted to optimize separation efficiency, allowing for effective control of separation performance. The Thermo Scientific Acclaim mixed-mode column features unique surface chemistry that facilitates easy adjustment of analyte elution, enabling maximum separation efficiency, which often leads to successful separation of compounds that cannot be addressed by other conventional high-performance liquid chromatography (HPLC) columns. The combination of ion exchange and reversed-phase, specifically HILIC chemistry, can extract the chemical properties of sample mixtures, allowing for complete separation of components in complex samples or high-sensitivity detection of trace samples. However, it is impossible to retain highly polar or charged analytes using only reversed-phase chromatography. Ion exchange (IEX) chromatography alone is insufficient for separating hydrophobic compounds. By using columns that combine different functionalities, a completely new toolkit is obtained for separating and evaluating samples.

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Cartridge column / Column for preparative HPLC and SFC

Cartridge column / Column for preparative HPLC and SFC

We have a wide variety available for use in both preparative HPLC and preparative SFC!

We offer cartridge-type columns suitable for flash chromatography. In addition to the FlashPure series filled with silica, C18, as well as amino and diol stationary phases, we have newly started selling Pure+Flash. Furthermore, our PrepPure columns for preparative HPLC and SFC feature unique selectivity for polar and non-polar, chiral and achiral compounds, with chemically bonded silica, as well as a lineup of coated or immobilized polysaccharides. 【Product Lineup】 ■ Cartridge columns for flash chromatography ・Pure+Flash ・FlashPure ■ Columns for preparative HPLC and SFC ・General-purpose columns ・Chiral separation columns *For more details, please download the PDF or feel free to contact us.

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