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Last Updated: Aggregation Period:May 06, 2026~Jun 02, 2026
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HPLC columns Product List

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Biomonolith

Designed for the separation of macromolecular biomolecules! Polymer-based monolith HPLC column.

We would like to introduce our bio-pharmaceutical analysis LC column, "Bio Monolith." It enables high-speed, high-resolution separation of antibodies (IgG, IgM), plasmid DNA, viruses, phages, and other macro biomolecules. Strong cation exchange phases, strong and weak anion exchange phases, and Protein A phases are available. It is compatible with HPLC and preparative LC systems such as the "1200 Infinity Series," as well as HPLC systems from other manufacturers. 【Features】 ■ Separation unaffected by flow rate ■ High-speed movement between the mobile phase and stationary phase ■ Ultra-fast separation ■ Accelerates method development and reduces costs by fixing method parameters *For more details, please refer to the related links or feel free to contact us.

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F5 (PFP) HPLC/UHPLC column

Separation of positional isomers using reverse phase and HILIC analysis! An additional step to add to C18 and HILIC columns.

The "F5 (PFP) HPLC/UHPLC column" for metabolomics is a reversed-phase column with selectivity different from C18. By incorporating it as a second or third choice column in reversed-phase systems, the range of substances for LC analysis is broadened. Four retention mechanisms—hydrogen bonding, dipole-dipole interactions, π-π interactions, and hydrophobic interactions—complexly influence each other to exhibit unique retention characteristics and stereoselectivity. 【Features】 ■ Retains highly polar basic compounds without ion-pair reagents ■ 100% water mobile phase is usable ■ Suitable for LC/MS due to retention at high organic solvent concentrations ■ Supports both analysis and fractionation *For more details, please refer to the PDF document or feel free to contact us.

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Acclaim Mixed-Mode HPLC Column

A column that is expected to provide high separation.

Liquid chromatography aims to achieve good separation ability to obtain meaningful information through peak resolution. In mixed-mode chromatography, numerous parameters can be adjusted to optimize separation efficiency, allowing for effective control of separation performance. The Thermo Scientific Acclaim mixed-mode column features unique surface chemistry that facilitates easy adjustment of analyte elution, enabling maximum separation efficiency, which often leads to successful separation of compounds that cannot be addressed by other conventional high-performance liquid chromatography (HPLC) columns. The combination of ion exchange and reversed-phase, specifically HILIC chemistry, can extract the chemical properties of sample mixtures, allowing for complete separation of components in complex samples or high-sensitivity detection of trace samples. However, it is impossible to retain highly polar or charged analytes using only reversed-phase chromatography. Ion exchange (IEX) chromatography alone is insufficient for separating hydrophobic compounds. By using columns that combine different functionalities, a completely new toolkit is obtained for separating and evaluating samples.

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Just 5 minutes of high-speed reverse-phase chiral separation ULTRON ES-OVM-3

Achieving ultra-fast chiral analysis using reverse-phase HPLC.

ULTRON ES-OVM-3 accelerates chiral separation and analysis using reverse-phase HPLC, a feature of ULTRON ES-OVM. It utilizes ovalbumin, an egg white protein, as a chiral recognition ligand, allowing for the chiral identification of many pharmaceuticals that dock with proteins. Chiral analysis of many pharmaceuticals, starting with Clopidogrel bisulfate, which utilizes ULTRON ES-OVM (USP L57), can be completed in 5 to 10 minutes. The following pharmaceuticals undergo ultra-fast reverse-phase chiral analysis in just under 5 minutes. For more details, please contact our company or your representative. - Clopidogrel bisulfate - Amlodipine - Atomoxetine hydrochloride - Carvedilol - Duloxetine hydrochloride - Tolperisone hydrochloride - Tolterodine

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High-performance columns for HPLC, UHPLC, and UPLC [CORTECS]

Improve productivity! A lineup of columns that maximizes lab performance.

This is the latest high-performance LC column family that maximizes the performance of laboratory LC systems. With high column efficiency, it achieves superior throughput and high separation compared to fully porous particle columns. It offers 1.6μm for UPLC, which realizes the ultimate theoretical plate count, and 2.7μm for HPLC/UHPLC, which is practical with low back pressure, allowing you to choose the optimal column for each analysis from a total of seven types of chemistry. Whether you want to separate complex samples or maintain separation while improving throughput, it delivers results beyond expectations. 【Features】 - Adoption of solid-core (core-shell) particles to maximize column efficiency - Particle size of 1.6μm for UPLC separation and 2.7μm for UHPLC/HPLC separation - A chemistry lineup that broadens selectivity for method development - Seven types of chemistry lineup: C18, C18+, T3, C8, Shield RP18, Phenyl, HILIC - Easy and seamless transfer of methods between 1.6μm and 2.7μm - Excellent reproducibility between batches and columns through comprehensive management of the entire manufacturing process from particle synthesis to final product

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ZORBAX Rx Reverse Phase HPLC Column

Rx-C18 is recommended for separation in low pH regions! It shows excellent stability and peak shape.

The "ZORBAX Rx Reverse Phase HPLC Column" is suitable for high-throughput analysis. It shows different selectivity compared to Eclipse XDB-C18 and StableBond SB-C18 in the low pH range, and StableBond is recommended for high-temperature applications. Additionally, Rx-C18 is manufactured using dimethyl octadecyl silane and demonstrates excellent stability up to pH 9 due to the lack of end-capping. 【Features】 ■ Shows different selectivity compared to Eclipse XDB-C18 and StableBond SB-C18 in the low pH range ■ Exhibits excellent stability and peak shape in low pH applications ■ Rx-C18 is manufactured using dimethyl octadecyl silane and demonstrates excellent stability up to pH 9 due to the lack of end-capping ■ Rx-C8 is the same product as SB-C8 *For more details, please refer to the PDF document or feel free to contact us.

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Silica-based column for HPLC/UHPLC 'ACE Generix'

A new product of a versatile column with excellent cost performance. Smooth replacement with other manufacturers' products is also possible.

"ACE Generix" is an HPLC/UHPLC column that maintains the traditional features of ACE columns, such as "high efficiency, high reproducibility, and long column life," while achieving high cost performance through thorough cost reduction. It uses high-purity spherical silica as its base, and the bonded phases include typical C18 types, as well as C8 types, Phenyl-Hexyl types, and SIL types in its lineup. It is available in a wide range of sizes, allowing for selection based on application. It is also easy to replace with products from other companies, contributing to cost reduction. 【Features】 ■ Manufactured at a UK facility certified with ISO9001 and ISO14001 ■ Exhibits sharp and symmetrical peak shapes against acids, bases, and neutral substances ■ Provides excellent reproducibility between columns and batches ■ Various guard columns are available to extend column life *You can view the English version of the catalog, which includes a comparison table with other products, by downloading the PDF. Please feel free to contact us for inquiries.

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HPLC Column "GPC Column - Molecular Weight Marker"

Introducing a wide range of products, including semi-micro SEC columns with a high degree of pore dispersion.

In addition to general-purpose GPC columns, we also introduce specialized SEC columns such as the ultra-fast SEC columns "SuperHZ Series" and "HXL Series," along with guidance on how to differentiate between SEC columns. 【Features】 <SuperMultiporeHZ Series> ■ Pore size-distributed semi-micro SEC column ■ Excellent linearity of calibration curves ■ No inflection points, resulting in undistorted chromatograms ■ Three grades available with different molecular weight fraction ranges *For more details, please refer to the PDF materials or feel free to contact us.

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HPLC/UHPLC column "FlexFire WP C4"

For the analysis of macromolecules such as proteins and antibodies! It greatly contributes to the analysis of biopharmaceuticals.

The "FlexFire WP C4" is a column designed to target macromolecules such as proteins and antibodies. To accommodate various analytical conditions, its pH and temperature durability have been significantly upgraded compared to previous models, greatly contributing to the analysis of biopharmaceuticals in the future. Additionally, it can also be applied to the analysis of intact mAbs, in addition to proteins. 【Features】 ■Elutes proteins with various properties based on hydrophobic differences, primarily aimed at analyzing primary structural characteristics. ■Applicable for the analysis of intact mAbs. ■Ensures durability at 80°C, allowing for sharp peak elution. *For more details, please refer to the PDF document or feel free to contact us.

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Core shell type column "SunShell C4-100"

Reverse phase column for the separation of monoclonal antibodies with a pore diameter of 1000A! Utilizing core-shell particles to achieve high theoretical plate numbers!

The "SunShell C4-100" is a core-shell type column for the separation of monoclonal antibodies. SunShell columns utilize core-shell silica and a specially modified Sunniest-bonding method, achieving efficient deactivation of silanol groups while also possessing high durability. Additionally, by using core-shell particles, the theoretical plate number per pressure increases, allowing for high separation to be easily achieved even with existing HPLCs that do not have high pressure tolerance. 【Features】 ■ Uses core-shell particles to achieve a high theoretical plate number ■ Usable in both HPLC and UHPLC with low pressure and high theoretical plate number ■ Sharp peak shapes due to excellent end-capping *For more details, please download the PDF or feel free to contact us.

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ACE UltraCore Column for HPLC and UHPLC

Core-shell column with a maximum pore size of 50 nm! Suitable for the analysis of large biomolecules such as proteins and peptides.

The "ACE UltraCore Column" is a biochromatography column that employs a core-shell (Solid-Core) design. With pore sizes of 30nm and 50nm (300Å and 500Å), it is suitable for the analysis of large biomolecules such as proteins and peptides over 5kDa. Leveraging the characteristics of the core-shell structure, it offers high separation capability comparable to UHPLC columns with particle sizes below 2μm, enabling fast analysis at low column pressure. 【Features】 ■ Uses ultra-pure and inert silica ■ Available in particle sizes of 2.5μm, 3.5μm, and 5μm ■ Available in pore sizes of 9.5nm, 30nm, and 50nm (95Å, 300Å, and 500Å) ■ Excellent reproducibility and long column life due to strict quality control ■ Various guard cartridge columns are also available ■ Stationary phases: C18, C4, CN, Phenyl2, SuperC18 (EBT), Phenyl-Hexyl *For more details, please refer to the PDF document (English version) or feel free to contact us.

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Ultra Hybrid UHPLC/HPLC Column

Durability and inertness that surpass conventional hybrid C18.

SunBridge is an all-porous hybrid column with unprecedented stability. Thanks to the fusion technology of silica surface treatment and the creation of organic silica particles (Ultra Hybrid Technology) that our company has developed since its founding, it exhibits extremely high durability and stability that surpasses the limits of conventional C18 columns. ★ Mobile phases from pH 1 to pH 12 can be used ★ Good peak shape for acidic, basic, and metal-coordinating compounds ★ A 40% discount campaign sale will be held from February 2025 to the end of July 2025 ★ Particle sizes of 3 µm and 1.8 µm are scheduled to be launched in the spring and summer of 2025

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Acclaim Surfactant Plus Column

For the analysis of consumables such as detergents, pharmaceuticals, food and beverages, and environmental samples! HPLC column.

The "Acclaim Surfactant Plus Column" is a state-of-the-art HPLC column with high efficiency and silica-based material. It allows for the simultaneous analysis of cationic surfactants, anionic surfactants, nonionic surfactants, and amphoteric surfactants. It can be used for the analysis of consumables such as detergents, pharmaceuticals, food and beverages, and environmental samples. 【Features】 ■ Selectivity suitable for the simultaneous analysis of cationic surfactants, anionic surfactants, nonionic surfactants, and amphoteric surfactants ■ Measurement of cationic surfactants ■ Separation of ethoxylated surfactants ■ Retention of highly hydrophilic compounds such as hydrotropes *Please feel free to contact us for more details.

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PLRP-S for biomolecules

Chemically and physically stable and robust! LC column for biopharmaceutical analysis.

"PLRP-S for Biomolecules" is an HPLC column filled with macroporous styrene-divinylbenzene. It is a highly reproducible reverse-phase HPLC column, and there is no need for the attachment of alkyl chains (such as C18 or C8) to enhance hydrophobicity. Additionally, the surface of PLRP-S has no unnecessary activity, so there is no occurrence of separation inconsistency due to residual silanol or end-capping rates seen in silica-based fillers. 【Features (Specifications)】 ■ Polymer: Macroporous spherical styrene-divinylbenzene ■ Particle sizes: 3μm, 5μm, 8μm, 10μm, 30μm ■ Pore sizes: 100Å, 300Å, 1000Å, 4000Å ■ Functional groups: None ■ Usable organic solvent concentration: 1–100% (100% water is not allowed) *For more details, please refer to the related links or feel free to contact us.

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HPLC column "Sunniest"

Developing a new combined phase! A column with an unprecedented end capping.

"Sunniest C18" uses a tri-functional reagent and features end-capping based on a unique new concept, making it the only one in the world. "Sunniest" significantly suppresses the influence of residual silanol groups, allowing for a wider range of conditions without tailing of basic compounds, and enabling the optimal setting of mobile phase pH and column temperature. 【Features】 ○ Almost no influence from silanol groups ○ Superior end-capping ○ The bonding reagent also has the effect of end-capping ○ RP-AQUA is prepared for stable separation with 100% aqueous mobile phase ○ Columns are available from an inner diameter of 2.0mm to 20mm for preparative use For more details, please contact us or download the catalog.

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Kemco Plus Automatic Boosting Slurry Filling Machine Model 124APP

You can easily fill HPLC columns.

This is a model that combines the conventional Kemco slurry filling machine model 124A with the new automatic pressure programmer PP-21. Column filling has been manually controlled for pressure, but due to individual differences, consistent quality has not always been achieved. The Kemco Plus automatic pressure slurry filling machine model 124APP solves all these issues and enables stable quality column filling without individual differences.

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VariTide RPC

Developing a polymer-based HPLC column to economically enhance throughput and analyze and purify synthetic peptides!

We would like to introduce our LC column for biopharmaceutical analysis, 'VariTide RPC.' To efficiently retain peptides composed of 5 to 60 amino acids, we adjust the particle size and pore size. It can be used at various pH ranges, allowing for the separation and purification of acidic, basic, hydrophilic, and hydrophobic peptides with this product. 【Features (Specifications)】 ■ Polymer: Macroporous spherical styrene-divinylbenzene ■ Particle size: 6μm ■ Pore diameter: 200Å ■ Functional groups: None ■ Column size: Inner diameter: 4.6mm to 21.2mm / Length: 25cm *For more details, please refer to the related links or feel free to contact us.

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DNAPac PA200/PA200 RS Column

High separation of oligonucleotide chains and stereoisomers! Anion exchange column.

The "DNAPac PA200 HPLC/DNAPac PA200 RS (High-Speed Separation) UHPLC Column" is a column designed for the high separation of ssDNA and RNA. The selectivity for oligonucleotides can be controlled by various parameters such as the pH of the mobile phase, solvents, and the use of different salts, allowing for adjustments in separation to meet the specific requirements of applications. We provide structural analysis and quantification methods utilizing high-resolution mass spectrometry and anion exchange columns for UHPLC. 【Features】 ■ High separation of oligonucleotide chains and stereoisomers ■ Improved separation and throughput ■ Control of selectivity and retention through the pH of the mobile phase, type of salt, and type of mobile phase ■ Separation of n from n-1 bases *For more details, please refer to the PDF document or feel free to contact us.

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Technical Data: Chiral Column for Reverse Phase Ultron ES-OVM

Effective for metabolic studies in blood! A column that is valuable in the fields of pharmaceuticals, pesticides, food, and organic synthesis.

The "Ultron ES-OVM" is an optical separation HPLC column that has many excellent features, such as being easy to use in reverse phase mode, having good peak shape, and being suitable for the study of trace metabolites. It uses amino-modified silica gel with proteins that are resistant to denaturation as the stationary phase, allowing for the analysis of plasma samples without any pre-treatment. This document includes the basic properties of the ligand, examples of separation degree adjustment, and case studies of compound analysis using the Ultron ES-OVM. We encourage you to read it. [Contents] ■ Basic properties of the ligand ■ Mechanism of separation ■ Selection of mobile phase ■ Relationship between pH and capacity factor ■ Relationship between salt concentration and capacity factor *For more details, please refer to the PDF document or feel free to contact us.

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ZORBAX Extend-C18

Improvement of retention, separation ability, and peak shape in base compounds! Introducing ZORBAX reverse phase HPLC columns.

The "ZORBAX Extend-C18" utilizes a groundbreaking bidentate C18-C18 bonding technology, enabling the development of high-separation methods in high pH regions while being a silica-based column. The unique bidentate bonding and double-end capping enhance stability at high pH. It improves retention, separation capability, and peak shape for basic compounds. To extend column life, it is preferable to use triethylamine, ammonium hydroxide, or pyrrolidine as buffer solutions, while phosphate buffers are not recommended. 【Features】 - High separation efficiency and long lifespan in high pH regions (up to pH 11.5) - Improved stability at high pH due to unique bidentate bonding and double-end capping - Higher efficiency and superior peak shape compared to polymer-based columns - High sensitivity and separation for LC/MS analysis of peptides and proteins *For more details, please refer to the PDF document or feel free to contact us.

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HPLC column for optical isomer separation

HPLC column for optical isomer separation

Optical isomers present in various bioactive substances (such as pharmaceuticals, pesticides, and fragrances) that are deeply related to our daily lives are known to exhibit significantly different biological activities between isomers, increasing the necessity for the analysis and separation of optical isomers. The "SUMICHIRAL OA series" HPLC columns for the separation of optical isomers are gaining attention in fields such as pharmaceuticals and pesticides.

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Analysis of peptides, proteins, and monoclonal antibodies by UHPLC.

A technical report featuring data primarily aimed at the analysis of biopharmaceuticals.

The document includes applications using "FlexFire WP C4," as well as the upcoming FlexFire WP C18 and WP C8. It primarily features data aimed at biopharmaceutical analysis, focusing on what can be achieved with wide-pore columns. Additionally, it includes data from other WP series. Please make use of it. [Contents] ■ Retention and separation due to differences in pore size ■ Analysis of peptides ■ Analysis of cyclic peptides ■ Analysis of proteins ■ Analysis of monoclonal antibodies *For more details, please refer to the PDF document or feel free to contact us.

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[Application Example] HPLC Analysis of Sugars Using the CarboSep Series

You can choose the column that suits you from a rich lineup.

Concise Separations, Inc. in the United States has been manufacturing and selling HPLC products for over 20 years. The CarboSep series supports various sugar analyses and is used by customers around the world. We have also included actual analysis examples in the case studies, so please take a look.

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HPLC column for organic acid analysis - 'Coregel Series'

You can choose from a rich lineup of columns for organic acid analysis.

Concise Separations, Inc. (formerly Transgenomic, Inc.) has been providing various HPLC columns for over 20 years. We offer the Coregel series as columns for organic acid analysis.

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HPLC Column "Reversed Phase / HILIC"

Numerous charts are included. In addition to the characteristics of each mode, we introduce the main target substances.

This document contains information on HPLC columns for normal phase chromatography and multi-mode chromatography. In addition to the characteristics of each mode, it introduces the main target substances. Furthermore, it includes charts and tables for separation and comparison. [Contents (excerpt)] ■ Normal Phase Chromatography ■ Multi-Mode Chromatography ■ Columns for LC/MS ■ Reverse Phase Chromatography *For more details, please refer to the PDF document or feel free to contact us.

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HPLC column "IEC・HIC・Affinity"

This is a book that explains the features and physical properties of ion exchange chromatography and other related topics.

This document explains the IEC, HIC, and affinity of HPLC columns. It introduces the characteristics of ion exchange chromatography columns, as well as basic physical properties. Additionally, it explains each product series using tables. Furthermore, it also includes information on hydrophobic chromatography and affinity chromatography. 【Contents (excerpt)】 ■ Ion Exchange Chromatography ■ Hydrophobic Chromatography ■ Affinity Chromatography *For more details, please refer to the PDF document or feel free to contact us.

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HPLC/UHPLC column "FlexFire C18"

Making HPLC⇔UHPLC more accessible! This is a column that uses refined silica gel.

The "FlexFire C18" is a highly versatile HPLC/UHPLC column that emphasizes hydrophobic interactions due to high carbon content. It provides results that are faithful to the fundamentals of HPLC (UHPLC) and offers important information to establish future directions. It allows for easy setting of analytical conditions, as it can be transferred between quality control and research & development according to the system. 【Features】 ■ Extremely versatile ■ Emphasizes hydrophobic interactions due to high carbon content ■ Available in 5μm, 2.6μm, and 1.6μm ■ Transferable between quality control and research & development according to the system ■ Utilizes refined silica gel *For more details, please refer to the PDF document or feel free to contact us.

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HPLC column "Ion Chromatography"

Explanation of separation and analysis using anion analysis columns, illustrated with diagrams.

This document explains ion chromatography of HPLC columns. It provides an explanation of the separation and analysis of both anion analysis columns and cation analysis columns, using diagrams and tables. 【Contents (excerpt)】 ■ Anion analysis column (for IC-2010) ■ Cation analysis column (for IC-2010) ■ Anion analysis column (non-suppressor type) ■ Anion analysis eluent ■ Cation analysis column (non-suppressor type) *For more details, please refer to the PDF document or feel free to contact us.

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HPLC column "Size Exclusion Chromatography"

Features of the SEC column and a list of compatibility with H-type solvents are included.

This document explains size exclusion chromatography (SEC) for HPLC columns. It includes features of SEC columns and a list of compatible H-type solvents. Additionally, the section on SEC column selection provides a detailed explanation of both aqueous SEC column selection (for synthetic polymers) and organic solvent-based SEC column selection, using tables for clarity. 【Contents (excerpt)】 ■ Features of SEC columns ■ List of compatible H-type solvents ■ SEC column selection ■ Aqueous SEC columns (for proteins, peptides, and nucleic acids) ■ Aqueous SEC columns (for synthetic polymers, polysaccharides, oligosaccharides, and nucleic acids) *For more details, please refer to the PDF document or feel free to contact us.

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