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column Product List

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Bio LC column

Bio LC column

High-resolution analysis of protein therapeutics.

Thermo Fisher Scientific's Bio LC columns separate biological samples with high resolution and efficiency. Our proprietary column chemical properties for biological samples are specifically designed for the separation of proteins, peptides, monoclonal antibodies, carbohydrates, nucleic acids, and more. - Protein HPLC columns - Peptide HPLC columns - Glycan HPLC columns - Oligonucleotide HPLC columns - Monoclonal antibody HPLC columns

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Column "Stainless Steel HPLC Empty Column"

Column "Stainless Steel HPLC Empty Column"

If you're having trouble with columns, please give it a try first!

The "Stainless Steel Empty Column for HPLC" is a column that can accommodate a wide variety of HPLC applications. We offer screw-type and ferrule-type (swagelok type) options, with a standard frit filtration precision of 2µ. Additionally, it boasts a wide range of inner diameters and high internal precision, and the column ends have low diffusion. Based on years of experience and proven results, we manufacture products that meet our customers' needs. 【Features】 ■ Compatible with a wide variety of HPLC applications ■ Extensive range of inner diameters ■ High internal precision ■ Low diffusion column ends ■ High precision *For more details, please refer to the catalog or feel free to contact us.

  • Column Chromatography
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Column "PEEK-made empty column for HPLC"

Column "PEEK-made empty column for HPLC"

Most organic solvents, acids, and salts used in HPLC are available.

The "PEEK-made empty column for HPLC" is a column compatible with most organic solvents, acids, and salts used in HPLC. The contact part is made of inert PEEK, so there is no adsorption/denaturation of the target components or rust formation. A pressure test with water confirmed retention at 400 MPa. Additionally, a filling test at 30 MPa has also been confirmed. The inner diameter standard is the same as that of stainless steel columns, with no variation in volume, and handling is the same as that of stainless steel columns. 【Features】 ■ Withstands water pressure up to 400 MPa ■ Passes the filling test at 30 MPa ■ No adsorption/denaturation of the target components or rust formation ■ Compatible with most organic solvents, acids, and salts *For more details, please refer to the catalog or feel free to contact us.

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ACE HILIC Column

ACE HILIC Column

A new HPLC column for hydrophilic interaction chromatography (HILIC) has been newly introduced in the ACE column series. Three exciting selectivities are available.

A new HPLC column for hydrophilic interaction chromatography (HILIC) has been introduced in the ACE column series, developed by ACT (Advanced Chromatography Technologies) and globally recognized for its high quality. It successfully facilitates the separation of highly polar substances that are not sufficiently retained in reverse phase mode. 【Features】 - Three uniquely developed stationary phase types - A simple and systematic approach to HILIC method development - Compatibility with HPLC/UHPLC systems from all manufacturers - Excellent robustness: withstands pressures up to 1,000 bar (15,000 psi) - Available in three particle sizes (1.7, 3, or 5 µm)

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Force Performance LC Column

Force Performance LC Column

Restek's 100% pure satisfaction guarantee! Compatible with high-pressure fast analysis using UHPLC.

Restek's "Force Performance LC Column" is a product that can increase productivity by maximizing the operating time of the equipment. It is designed to easily transfer methods from HPLC to advanced UHPLC and to withstand high pressure and severe pressure fluctuations. Whether using columns with particle sizes of 3μm or 5μm in a typical HPLC system, or using columns with a particle size of 1.8μm in a new UHPLC, it consistently provides the same separation. 【Features】 ■ Durability and reproducibility ■ Compatibility between HPLC and UHPLC ■ High quality *For more details, please refer to the PDF document or feel free to contact us.

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Column "ZORBAX NH2"

Column "ZORBAX NH2"

ZORBAX offers both bonded and non-bonded series of stationary phases for reversed-phase HPLC columns!

"ZORBAX NH2" is a packing material that combines propylaminosilane with "ZORBAX SIL." It is used for normal phase separation, weak anion exchange, and reverse phase HPLC of polar compounds. When using this product, vitamins A and D are separated in normal phase mode, while hydrocarbons and sugars are separated in reverse phase mode. [Features] - Packing material that combines propylaminosilane with ZORBAX SIL - Used for normal phase separation, weak anion exchange, and reverse phase HPLC of polar compounds - Vitamins A/D are separated in normal phase mode, while hydrocarbons and sugars are separated in reverse phase mode *For more details, please refer to the related links or feel free to contact us.

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F5 HPLC/UHPLC column

F5 HPLC/UHPLC column

Separating structural isomers and related compounds through multiple interactions (MIX mode)!

We would like to introduce our "F5 HPLC/UHPLC Columns." These columns retain high-polarity basic compounds without the use of ion-pair reagents. They can separate related compounds such as metabolites. Additionally, they improve MS sensitivity by retaining compounds at high organic solvent concentrations, and 100% water mobile phase solvents can be used. 【Features】 ■ Retains high-polarity basic compounds without ion-pair reagents ■ 100% water mobile phase solvents can be used ■ Improves MS sensitivity by retaining compounds at high organic solvent concentrations ■ Can separate related compounds such as metabolites *For more details, please refer to the PDF document or feel free to contact us.

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Column "Pursuit XRS/XRS Ultra"

Column "Pursuit XRS/XRS Ultra"

The ultra-high purity base silica increases efficiency by 10-15% compared to a 3μm column!

The "Pursuit XRS/XRS Ultra" is a column that shortens analysis time while maintaining separation capability in general HPLC and LC/MS systems. The Agilent HPLC column series has been expanded with the addition of the "Pursuit XRs Ultra 2.8," an LC column with a particle size of 2.8μm. You can achieve rapid measurements while maintaining high separation efficiency with your current HPLC system. 【Features】 ■ Rapid analysis ■ High efficiency ■ High pressure resistance - Usable in general LC systems up to 1000 bar (=1020 kgf/cm2) *For more details, please refer to the related links or feel free to contact us.

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Column "AdvanceBio Peptide Mapping"

Column "AdvanceBio Peptide Mapping"

The particle size of 2.7μm allows for UHPLC performance in HPLC systems!

"AdvanceBio Peptide Mapping" is an LC column designed for biopharmaceutical analysis that provides high-resolution peptide maps for protein identification and post-translational modification measurement. Using this product, amino acid substitutions/modifications in the protein primary sequence can be rapidly separated and identified. This surface-porous advanced bio-column has a pore size of 120 Å, making it suitable for the analysis of peptides generated by enzymatic digestion of proteins. 【Features】 ■ Each batch is verified for stability and reproducibility using peptide standard substances. ■ Reliable analytical data can be obtained. ■ Identification of major peptides in complex peptide maps is possible. ■ UHPLC performance can be achieved with general HPLC equipment with a maximum pressure of 400 bar. ■ MS sensitivity is improved when using formic acid as the mobile phase in HPLC. *For more details, please refer to the PDF document or feel free to contact us.

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Column "ZORBAX Eclipse Amino Acid Analysis (AAA)"

Column "ZORBAX Eclipse Amino Acid Analysis (AAA)"

The test for amino acid analysis (AAA) is complete! Using the general OPA and FMOC derivatization methods.

The "ZORBAX Eclipse Amino Acid Analysis (AAA)" is a column designed for high-speed separation of amino acids, following updated and improved protocols for use with Agilent HPLC systems. All analytical tasks between injections can be completed in 14 minutes (with a 10-minute analysis time) using a short 7.5 cm column, or in 24 minutes (with a 16-minute analysis time) using a long 15 cm column. By utilizing the OPA and FMOC derivatization methods in a fully automated single procedure with the Agilent HPLC system, very high sensitivity (5-50 pmol with DAD and FLD) and reliability are achieved. 【Features】 ■ High-resolution, high-speed analysis of 24 types of amino acids ■ Tested for amino acid analysis ■ Easily automated with detailed online derivatization protocols, compatible with Agilent HPLC autosamplers *For more details, please refer to the related links or feel free to contact us.

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Recycling Fractionation HPLC LaboACE

Recycling Fractionation HPLC LaboACE

This is a preparative HPLC aimed at the isolation and purification of target compounds.

The highly acclaimed LaboACE series has further evolved, with significantly improved recovery rates and purity. With a combination of comprehensive automatic functions and high-speed preparative GPC columns, you can rely on us for large-scale preparative needs! You can program up to 30 tasks (events) for a single chromatogram, including sample injection, cutting unnecessary peaks, recycling, and fractionation, and repeat the entire sequence up to 99 times. Additionally, the built-in fraction collector in the main unit can accommodate various container sizes, making it ideal for large-scale fractionation of the same sample. 【Features】 - Compact body equipped with comprehensive automatic functions - Standard recycling function that promotes separation by repeatedly passing through the column (no solvent is consumed during recycling). - Automatic cleaning function for automatic flow path cleaning - Easy operation with a large LCD touch panel - PC control function (optional) to operate the main unit via the included PC - Low-pressure two-liquid gradient capability is also available (optional) *Manual models are also available in the lineup. For more details, please feel free to contact us.

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Column "Pursuit UPS"

Column "Pursuit UPS"

Even in measurements requiring high speed and high separation, it can be performed using general HPLC or LC/MS!

"Pursuit UPS" is an LC column designed for high-speed analysis using your existing HPLC or LC/MS systems without the need for specialized equipment. By controlling the particle size to 2.4μm, it enables analysis that is 30-35% more efficient compared to columns with a particle size of 3μm. Additionally, it can reduce back pressure to about half compared to columns with a particle size of 2μm or less, allowing for rapid analysis with your already used LC systems. 【Features】 ■ Improved work efficiency - Compared to traditional 3μm particle size columns, it reduces peak retention time and peak width by 30-35%. *For more details, please refer to the related links or feel free to contact us.

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Raptor Core Shell LC Column

Raptor Core Shell LC Column

Sustainable reproducibility, diverse stationary phases and particle sizes! A rich lineup.

Introducing Restek's "Raptor Core-Shell LC Column." It features high efficiency that significantly reduces analysis time, improved selectivity that enhances resolution, and the potential for improved sample throughput with existing HPLC and UHPLC systems. We offer various types including Biphenyl, ARC-18, and C18. Please feel free to contact us if you have any requests. 【Features】 ■ High efficiency that significantly reduces analysis time ■ Improved selectivity that enhances resolution ■ Improved sample throughput with existing HPLC and UHPLC systems ■ Sustainable reproducibility ■ A variety of stationary phases and particle sizes *For more details, please refer to the PDF document or feel free to contact us.

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Low-adsorption LC column MaxPeak Premier column

Low-adsorption LC column MaxPeak Premier column

Effectively reduce non-specific adsorption (NSA) of compounds that easily adsorb to metals, providing excellent durability and consistency over a long period.

This is a low-adsorption column that employs MaxPeak HPS technology, which minimizes the interaction between the analyte and column hardware (column tube) that can cause sample loss, thereby improving the recovery rate, sensitivity, and reproducibility of the analyte. MaxPeak HPS technology is an advanced surface treatment technology rather than a resin coating. It is designed to withstand the rigorous conditions of high-throughput laboratories, providing excellent performance that lasts without compromise. 【Features】 - Reduces the effort and time required for column conditioning and passivation - Improves sensitivity and peak shape - Allows analysis with a simpler mobile phase that does not contain complex additives - Saves time in method development - Reduces risk and enhances reliability in data and decision-making - A lineup compatible with all LC systems including UPLC/UHPLC/HPLC

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InfinityLab Poroshell 120 Column

InfinityLab Poroshell 120 Column

Available in three particle sizes! You can choose the particle size according to your separation needs.

The "InfinityLab Poroshell 120 Column" provides suitable analytical efficiency and reliability, significantly enhancing the performance of various instruments. It is suitable for those seeking superior HPLC performance or wishing to achieve UHPLC performance at lower pressures, as well as for those looking for the highest level of UHPLC performance. Available in three particle sizes: 1.9μm, 2.7μm, and 4μm, you can choose the particle size according to your separation needs. Additionally, it is compatible with almost all LC systems and offers 12 types of packing materials, allowing you to select from a wide range of columns with diverse selectivity, making method development quick and easy. [Features] - Significantly improves the performance of various instruments - Suitable for those seeking the highest level of UHPLC performance - Available in three particle sizes: 1.9μm, 2.7μm, and 4μm - Quick and easy method development *For more details, please refer to the PDF document or feel free to contact us.

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ZORBAX RRHD Column

ZORBAX RRHD Column

It shows the same selectivity as the 3.5 and 5µm ZORBAX columns using the same stationary phase!

The "ZORBAX Rapid Resolution High Definition (RRHD) column" is a product that further expands the lineup of ZORBAX columns with a particle size of 1.8µm. Through the optimization of the packing process, it achieves a pressure tolerance and stability of up to 120MPa (1200bar), suitable for use with the Agilent 1290 Infinity LC and other high-pressure HPLCs. Columns of lengths 50, 100, and 150mm are available, enabling the analysis of very complex samples at high speed or high separation. 【Features】 ■ Ultra-high pressure (120MPa [1200bar]) columns capable of achieving optimal results with the 1290 Infinity LC or other high-pressure HPLC systems ■ Achieves top-class separation ability with a particle size of 1.8µm ■ Two types of stationary phases are available: ZORBAX Eclipse Plus C18 for excellent peak shape, and ZORBAX StableBond C18 for superior selectivity and stability at low pH *For more details, please refer to the PDF document or feel free to contact us.

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PepSep column

PepSep column

Excellent robustness and reproducibility, compatible with ion sources from various manufacturers.

The "PepSep Column" is a column suitable for the separation of peptides. We offer a lineup that covers various proteomics applications, allowing us to meet all of the user's chromatography needs. It can be used with various UHPLC systems, and the connection with HPLC systems and emitters can be easily made. 【Lineup】 ■PepSepULTRA ■PepSepXTREME ■PepSepMAX *For more details, please download the PDF or feel free to contact us.

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Column "MicroBore"

Column "MicroBore"

When the sample mass is the same, the detection limit is improved by five times compared to a column with an inner diameter of 2.1 mm!

The "MicroBore" is a column suitable for the analysis of trace samples. This product uses low flow rates (generally below 50 μL/min). Therefore, it is suitable for use with detectors that require low flow rates, such as some mass spectrometers. The guard column can be adjusted for the depth of the tube stop, allowing for a complete zero dead volume connection at all times. 【Features】 ■ Suitable for use in HPLC systems purchased or modified for trace analysis ■ Achieves high sensitivity even with small sample volumes ■ Compatibility with LC/MS interfaces ■ A wide variety of stationary phases available ■ Guard columns that can be used with MicroBore columns (inner diameter 1.0 mm) and capillary columns *For more details, please refer to the related links or feel free to contact us.

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Column "Ultron ES"

Column "Ultron ES"

Acetonitrile, ethanol, and phosphate buffer can be used as mobile phases!

"Ultron ES" is an insolubilized protein column equipped with asymmetric recognition sites for the optical isomer separation of numerous chiral compounds. It is designed to have a chiral stationary phase with two complementary protein bases, making it suitable for HPLC separation of optical isomers without derivatization. Additionally, it is suitable for the separation of pharmaceuticals. It can directly separate racemates without going through derivatization. 【Features】 ■ Ultron ES-OVM is used for the separation of optical isomers of acidic and basic pharmaceuticals. ■ Basic compounds that are difficult to separate with common products can sometimes be easily separated. ■ The ES-OVM column and ES-Pepsin column use a 120Å, 5μm silica packing that combines ovomucoid protein and pepsin protein. *For more details, please refer to the related links or feel free to contact us.

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Column "ZORBAX 300Extend-C18"

Column "ZORBAX 300Extend-C18"

High robustness and separation of polypeptides and peptides over a wide range of pH from high pH to low pH (pH 2 to 11.5)!

The "ZORBAX 300Extend-C18" is a wide-bore HPLC column that efficiently separates peptides in the pH range of 2 to 11.5. Its unique bidentate bonding phase provides excellent longevity and reproducibility in both high and low pH regions. The retention characteristics and selectivity for peptides and polypeptides in the high pH region significantly change as these molecules become charged. 【Features】 ■ Shows different selectivity at high and low pH ■ Achieves excellent efficiency and good recovery rates for hydrophobic peptides at high pH ■ Suitable for LC/MS analysis using ammonium hydroxide mobile phase *For more details, please refer to the PDF document or feel free to contact us.

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Raptor LC Column

Raptor LC Column

A variety of stationary phases and particle sizes! LC columns with better selectivity that improve separation efficiency.

Restek's "Raptor LC Column" is a highly efficient LC column that significantly reduces analysis time. It includes options suitable for biological sample analysis, such as the "Raptor Biphenyl Column" for drug and metabolite analysis, as well as the "Raptor ARC-18 Column." It offers better selectivity that improves resolution and sustainable reproducibility. 【Features】 ■ High efficiency that significantly reduces analysis time ■ Better selectivity that improves resolution ■ Improved sample throughput with existing HPLC and UHPLC ■ Sustainable reproducibility ■ A variety of stationary phases and particle sizes *For more details, please refer to the PDF document or feel free to contact us.

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Column "Hi-Plex"

Column "Hi-Plex"

High efficiency and low operating pressure! Separation of carbohydrates and alcohols is possible through ligand exchange.

"Hi-Plex" is a column that achieves high efficiency, low operating pressure, and long column life due to its monodisperse materials. With a series of ligand-to-ion pairs that provide suitable selectivity, resolution, and materials corresponding to the USP definitions for filler types "L17/19/34" and "L58," this product is ideal for isocratic separation using water or diluted acids as solutes. This simplifies HPLC system requirements and eliminates the need to use hazardous organic solvents. [Features] ■ Resolution and materials corresponding to the USP definitions for filler types L17/19/34 and L58 ■ Separation of carbohydrates and alcohols through ligand exchange ■ Separation of organic acids through ion exchange *For more details, please refer to the related links or feel free to contact us.

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カラム『AdvanceBio オリゴヌクレオチド』

カラム『AdvanceBio オリゴヌクレオチド』

独自のテクノロジーを使用！高pH移動相に対する耐性を大幅に高めている

『AdvanceBio オリゴヌクレオチド』は、高効率、2.7μmの表面多孔質 Poroshell粒子が特長のバイオ医薬品分析用LCカラムです。 エンドキャップ処理を施したC18相で オリゴヌクレオチドの選択性を高めます。 さらに、分離能標準を用いて当製品をバッチテストすることで、 一貫した性能を実現しています。 【特長】 ■HPLCおよびUHPLCシステムの長寿命カラムによる、 　オリゴヌクレオチドの高分解能分離 ■結果の信頼性の向上 ・Poroshellの効率的な粒子形態により高分解能分離を実現 ■コストの削減 ・高pHに対して優れた耐性を持つ堅牢な化学修飾シリカによりカラムを長寿命化 ※詳しくはPDF資料をご覧いただくか、お気軽にお問い合わせ下さい。

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Agilent BioHPLC Column

Agilent BioHPLC Column

If all columns and consumables are used correctly, the lab work will proceed smoothly!

The "Agilent BioHPLC Column" is designed to perform high-precision and high-speed characterization analysis of monoclonal antibodies and other intact proteins, aggregates by SEC, charge variants by IEX, intact mass by reversed phase, primary structure, post-translational modifications (PTM), and cleaved glycan analysis by hydrophilic interaction liquid chromatography. To further enhance the productivity of chromatography labs, smooth operations can be achieved if all columns and consumables are used correctly. 【Features】 ■ Achieves fast and consistent biopharmaceutical analysis ■ Designed to provide consistent performance ■ Underlying technology of the column improves accuracy and productivity ■ Supports speeding up analysis and enhancing lab productivity *For more details, please refer to the PDF materials or feel free to contact us.

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CHEMCO MASK-ENV Column

CHEMCO MASK-ENV Column

High-performance pre-treatment column to prevent clogging of the column by humic acid.

The CHEMCO MASK-ENV column is a high-selectivity column developed for the pretreatment of environmental water samples in column switching methods. By introducing hydrophilic functional groups (sulfonic acid polymer) near the surface of a hydrophobic polymer filler through the MASK modification method, efficient concentration of target analytes and cleanup of interfering components can be achieved.

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Column "AdvanceBio MS Spent Media"

Column "AdvanceBio MS Spent Media"

Rapid MS-based workflow! No need for sample derivatization, saving time and resources.

The "AdvanceBio MS Spent Media" is a HILIC column that can separate non-derivatized amino acids and other polar metabolites in cell culture media with high speed, excellent sensitivity, and reproducibility, making it suitable for mass spectrometry. By combining it with Agilent InfinityLab LC series instruments and Agilent MS instruments, a comprehensive solution for cell culture component analysis is achieved. The AdvanceBio MS Spent Media analysis is an innovative solution designed for the generation and characterization of biomolecules, as a new product in the Agilent AdvanceBio family. 【Features】 ■ Achieves excellent peak shape and recovery rates even for challenging ionic metabolites ■ Baseline chromatographic separation capability for isomers of leucine and isoleucine ■ Columns tested with amino acids to ensure quality and performance ■ Excellent analytical sensitivity with a mobile phase suitable for MS ■ Compatible with both HPLC and UHPLC systems *For more details, please refer to the PDF document or feel free to contact us.

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InfinityLab Poroshell 120 Column

InfinityLab Poroshell 120 Column

Method development is quick and easy! Compatible with almost all LC systems, with 12 types of fillers available.

The "InfinityLab Poroshell 120 Column" enhances analytical efficiency and reliability, significantly improving the performance of various instruments. Whether you desire superior HPLC performance, want to achieve UHPLC performance at lower pressures, or are seeking the highest level of UHPLC performance, this column is suitable for you. Available in three particle sizes: 1.9μm, 2.7μm, and 4μm, allowing you to choose the appropriate particle size based on your separation needs. 【Features】 ■ A column with innovative chiral stationary phase on porous surface particles ■ High-speed, high-separation column that increases throughput and separation capability ■ Excellent inter-batch reproducibility that consistently delivers reliable separation results ■ Selectable particle sizes Particle sizes of 1.9μm, 2.7μm, and 4μm maximize the performance of methods and instruments *For more details, please refer to the PDF document or feel free to contact us.

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Glycan Separation Technology Column

Glycan Separation Technology Column

It is optimal for UPLC analysis of fluorescent (2-AB) labeled glycans with a stable amide column.

Glycoprotein analysis requires the identification of complex N-linked and O-linked structures consisting of repetitive similar sugar components. Hydrophilic interaction chromatography (HILIC) utilizing fluorescence detection is a highly reliable analytical method that effectively separates and quantifies glycan chains labeled with fluorescent labeling reagents. The Waters Glycan Separation Technology column is a dedicated QC-tested column developed for the rapid analysis of various glycan structures with excellent UPLC(R) separation capability. By combining the Waters ACQUITY UPLC(R) system with column chemistry, accurate analytical results can be obtained more quickly. 【Features】 ○ Shorter time and higher separation capability compared to conventional HPLC methods ○ Ideal for use with the ACQUITY UPLC fluorescence detection system ○ Stable and reproducible separation of labeled glycan chains Waters BEH particles and functional group bonding technology ○ QC tests using labeled glycan standards are conducted to ensure consistent column performance between batches For more details, please contact us or download the catalog.

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ZORBAX column / Poroshell column

ZORBAX column / Poroshell column

Dramatic improvement in productivity! Flexibility and scalability of methods for low molecular weight and biomolecule analysis.

The "ZORBAX Columns / Poroshell Columns" are specially designed products for HPLC analysis with high productivity. The silica used is of extremely high purity, very robust, and highly uniform, providing the highest level of reliability. Additionally, one of the features is the extremely wide range of phases and column configurations that cater to diverse application needs. 【Features of ZORBAX Columns】 ■ Excellent options and flexibility for low molecular weight and reversed-phase biomolecule analysis with over 18 phases, including Eclipse Plus and HILIC ■ Easy method development that demonstrates excellent performance across a wide range of target compounds and conditions ■ Improvement of UHPLC methods ■ Outstanding peak shape performance due to proven innovative silica and bonding technology ■ Long-term reliability by having the necessary options available when needed for analysis and preparative purification *For more details, please refer to the PDF materials or feel free to contact us.

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