![[Case Study] On the Use of PNA in Amplicon Sample Preparation](https://image.mono.ipros.com/public/product/image/2ef/2000603360/IPROS20301676918714465824.png?w=280&h=280)
[Case Study] On the Use of PNA in Amplicon Sample Preparation
It is possible to estimate higher resolution feed organisms! Introduction of next-generation sequencing analysis examples *Analysis case collection is being distributed.
In our company, when preparing amplicon samples aimed at analyzing the microbial community structure present in plant leaves, we add PNA to suppress amplification from plant mitochondrial and chloroplast origins, resulting in favorable outcomes. Without the addition of PNA, approximately 90% of the acquired data consists of amplification products derived from the host. By adding PNA, we can reduce this to about 10%, allowing for a higher resolution estimation of the target organisms. [Results of condition testing with 4 sample units] ■ Ct1-Ct4: No PNA added ■ Mt1-Mt4: PNA added for mitochondrial blocking ■ Pl1-Pl4: PNA added for chloroplast blocking ■ PM1-PM4: PNA added for both mitochondrial and chloroplast blocking *For more details, please refer to the PDF document or feel free to contact us.
- Company：ファスマック
- Price：Other
