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reagent Product List and Ranking from 111 Manufacturers, Suppliers and Companies | IPROS GMS

Last Updated： Aggregation Period:Feb 11, 2026～Mar 10, 2026
This ranking is based on the number of page views on our site.

reagent Manufacturer, Suppliers and Company Rankings

Last Updated： Aggregation Period:Feb 11, 2026～Mar 10, 2026
This ranking is based on the number of page views on our site.

  1. ビジョンバイオ Fukuoka//Testing, Analysis and Measurement
  2. ファルマ Tokyo//others
  3. KANTO CHEMICAL CO., INC. Biochemical Dept Tokyo//Chemical
  4. 4 null/null
  5. 5 ニッポンジーン Toyama//others
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reagent Product ranking

Last Updated： Aggregation Period:Feb 11, 2026～Mar 10, 2026
This ranking is based on the number of page views on our site.

  1. New fixing solution "Artefix" that does not contain formaldehyde. ファルマ
  2. Foreign Substance Identification Group (Bloodstain Identification Reagent) [Simple determination by just dropping the reagent] ビジョンバイオ
  3. Reagent: Karl Fischer Reagent Chem Aqua
  4. 4 Amino Acid Analyzer Reagents PF-KANTO/PH-KANTO Series KANTO CHEMICAL CO., INC. Biochemical Dept
  5. 5 Powder Artificial Gastric Juice Artificial Intestinal Juice BioWise アイビック・リサーチ

reagent Product List

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Monomer for polyimide synthesis

Monomer for polyimide synthesis

We have a variety of raw materials (monomers) available for synthesizing heat-resistant, chemical-resistant, and electrically insulating polyimides.

Tokyo Chemical Industry offers a range of p-type organic semiconductors, n-type organic semiconductors, and bipolar organic semiconductors for organic transistors. 【Products】 ■ Aliphatic amines ■ Aromatic amines ■ Aliphatic carboxylic acid anhydrides ■ Aromatic carboxylic acid anhydrides ■ Aliphatic isocyanates ■ Aromatic isocyanates

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  • Engineering Plastics
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[Information] Novabiochem(R) Peptide Synthesis Reagent Product Guide

[Information] Novabiochem(R) Peptide Synthesis Reagent Product Guide

Setting strict product standards for 20 types of standard Fmoc amino acids!

This catalog is a product guide for the high-quality brand of peptide synthesis reagents, "Novabiochem(R)." It sets strict product standards for standard Fmoc amino acids used in peptide synthesis. You can use it with confidence for everything from basic research to pharmaceuticals, cosmetics, and contract synthesis. We offer a wide range of packaging options, from small sizes to bulk sizes. [Contents (excerpt)] ■ Major Fmoc amino acids ■ Pseudoproline dipeptides ■ Coupling agents ■ Resins for solid-phase synthesis ■ History of Novabiochem innovations *For more details, please refer to the PDF document or feel free to contact us.

  • Other polymer materials
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Brighter LC/MS: Solvent, mobile phase additives, reagents

Brighter LC/MS: Solvent, mobile phase additives, reagents

A wide range of solvents for LC/MS, additives for mobile phases, and reagents are featured!

This catalog features a wide range of LC/MS solvents, additives for mobile phases, and reagents. Liquid Chromatography-Mass Spectrometry (LC/MS) has become a routine analysis method, requiring high-purity reagents, mobile phases, and additives for mobile phases for sample preparation. Additionally, it is essential to always consider the risk of contamination from reagents, solvents, and devices used in sample preparation. Our company offers a rich selection of solvents, mobile phase additives, and reagents suitable for LC/MS. [Contents] ■ Solvents for LC/MS, blended solvents ■ Reagents and additives for LC/MS ■ Derivatization reagents for LC/MS ■ LC/MS methods for impurity profiling *For more details, please refer to the PDF document or feel free to contact us.

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[Information] Sigma Aldrich Fluorination Reagent Product Guide

[Information] Sigma Aldrich Fluorination Reagent Product Guide

Featuring highly thermally and chemically stable deoxygenated fluorines such as "PyFluor" and "DMPU-HF"!

This document is a product guide for "fluorinated reagents" handled by our company. We introduce "Baran Diversinates," which allows for the addition of various functional groups, such as fluorinated alkyl groups, onto aromatic heterocyclic frameworks with simple operations that are not affected by moisture or the atmosphere, as well as "XtalFluor," a stable solid fluorination reagent that is easy to handle. Please use this guide to assist in selecting products. [Contents (excerpt)] ■Baran Diversinates: Introduction of fluorine to aromatic heterocyclic frameworks ■PhenoFluor, PhenoFluor MIX, AlkylFluor: User-friendly deoxygenative fluorination agents ■PyFluor: Deoxygenative fluorination agent with high thermal and chemical stability ■DMPU-HF: A new nucleophilic fluorination reagent *For more details, please refer to the PDF document or feel free to contact us.

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Catalog of reagents related to histology and hematology research

Catalog of reagents related to histology and hematology research

Starting with 'cell separation-related reagents,' it includes the selection of 'appropriate density gradient solutions (media)' and more.

This catalog introduces reagents related to histology and hematology research. It includes "cell separation-related reagents," as well as "Histopaque density gradient media," "the basics of centrifugation," and "selection of appropriate density gradient solutions (media)." We encourage you to read it. 【Contents (excerpt)】 ■ Cell separation-related reagents ■ Histopaque density gradient media ■ The basics of centrifugation ■ Various centrifugation methods *For more details, please refer to the PDF document or feel free to contact us.

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Merck Microscope Reagent Catalog 2023

Merck Microscope Reagent Catalog 2023

A diverse lineup of products including staining solutions for organization, blood smears, and staining solutions for blood cell count determination.

This catalog introduces the reagents for microscopes that we handle. It includes various products such as staining solutions for cytology, staining solutions for bacteria and microorganisms, and staining solutions for fiber examination. In addition, we also provide information on dyes, chromogenic substrates, fluorescent dyes, and fixatives, so please take a moment to read through it. [Contents (excerpt)] ■ Regarding orders ■ CE mark and others ■ Staining solutions for tissues ■ Staining solutions for cytology ■ Staining solutions for blood enhancement and cell count ■ Staining solutions for bacteria and microorganisms * The information contained in this catalog is as of 2023. * For more details, please download the PDF or feel free to contact us.

  • Staining Reagents
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PET business

PET business

Short delivery time! Available for sale with a purity of over 98%, starting from a minimum quantity of 10mg.

Nard Institute was founded in 1972 starting from contract research business and began custom synthesis of PET reagents in 1995. For PET reagents, we have a wealth of synthesis achievements for numerous precursors and reference materials. Our company offers a wide range of products, available for sale with a purity of 98% (or 95%) or higher, starting from a minimum quantity of 10mg. We provide short delivery times and will attach HPLC charts with results. Product information is constantly updated, so please feel free to consult us. 【Features】 ■ Custom synthesis for reagents not listed in the catalog ■ Extensive manufacturing experience compliant with GMP ■ Manufacturing support from our subsidiary Nard Chemicals, which has a quality assurance system based on GMP standards ■ Design of compound structures suitable for PET probes and synthesis of derivatives to support early development of PET probes *For more details, please refer to the related links page or feel free to contact us.

  • Other contract services
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Pneumococcal identification reagent "Sodium deoxycholate solution"

Pneumococcal identification reagent "Sodium deoxycholate solution"

No need for labor-intensive concentration adjustment work, and dripping work is also easy. Inspection reports can also be issued.

We provide a "sodium deoxycholate solution" that can be used for bile solubility tests to identify Streptococcus pneumoniae. The liquid reagent is pre-adjusted for concentration, eliminating the need for cumbersome concentration adjustments. The container uses a dropper bottle, making the dropping process easy. By using the bile solubility test in conjunction, more accurate species identification is possible. 【Features】 ■ Two concentrations available: 2% and 10% ■ Enables bile solubility tests for identifying Streptococcus pneumoniae ■ Pre-adjusted concentration as a liquid reagent ■ Inspection report can be issued *You can view the product introduction materials and a comprehensive catalog featuring many products we handle by clicking the download button below.

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Alpha-glucosidase reagent

Alpha-glucosidase reagent

By conducting the α-glucosidase test, it is possible to differentiate between the genera Bacteroides and Fusobacterium!

This product is a simple enzyme reaction reagent that can differentiate between the genera Bacteroides and Fusobacterium. Positive bacterial species include Bacteroides fragilis, Bacteroides thetaiotaomicron, Parabacteroides distasonis, Phocaeicola vulgatus, and Prevotella bivia. Negative bacterial species include Porphyromonas asaccharolytica, Fusobacterium nucleatum, Bilophila wadsworthia, and Desulfovibrio desulfuricans. 【Instructions for Use (Using Microtubes)】 1. Add 20-50 μl of sterile water to the microtube. 2. Use a toothpick or similar tool to take an appropriate amount of colony and suspend it to create a concentrated bacterial solution. 3. Add an equal amount of α-glucosidase reagent to the suspension and mix. 4. Incubate at 35°C to 37°C for 10 to 15 minutes. 5. Shine UV light to check for fluorescence (blue-white) presence. *For more details, please download the PDF or feel free to contact us.

  • Other research reagents
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Antibiotic-resistant bacteria detection reagent "Boric acid solution"

Antibiotic-resistant bacteria detection reagent "Boric acid solution"

Compare the inhibition zones! Determine the production of beta-lactamases and carbapenemases.

We would like to introduce our resistant bacteria detection reagent, "Boron Acid Solution." Using disks for susceptibility testing, we compare the inhibition zones obtained from disks with and without the addition of this product to determine the production of beta-lactamase and carbapenemase. After culturing at 35°C overnight (16 to 18 hours), if an expansion of the inhibition zone diameter (generally 5mm or more) is observed due to the addition of boron acid, it is judged as positive. 【Product Lineup】 ■ SG-BA50-5 (50μL: 5 pieces) ■ SG-BA50-10 (50μL: 10 pieces) ■ SG-BA50-20PB (50μL: 20 pieces) ■ SG-BA50-50PB (50μL: 50 pieces) *For more details, please download the PDF or feel free to contact us.

  • Other research reagents
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Realization of acceleration, high sensitivity, and simplification in the analysis of N-type glycan characteristics.

Realization of acceleration, high sensitivity, and simplification in the analysis of N-type glycan characteristics.

Innovative technology for the characterization of glycoproteins.

■Extraction of glycans from glycoproteins, labeling, and purification can be completed in just three steps in 30 minutes. This dramatically improves the throughput of glycan analysis. ■Thanks to a significant increase in sensitivity, reliable glycan analysis is possible even with trace amounts of glycoproteins. Glycan identification is achievable from as little as 0.1 μg of glycoprotein. ■By combining a fluorescence detector with MS, both quantification and mass information of trace glycans can be obtained simultaneously in a single analysis. This enables reliable and rapid glycan analysis. ■The GlycoWorks HILIC μElution plate allows for easy and rapid purification. No more drying down is necessary. ■Everything needed for glycan extraction, labeling, and purification is packaged together. You can start preparing glycans right away.

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Test reagent: Frozen S-9 for mutagenicity testing

Test reagent: Frozen S-9 for mutagenicity testing

Introducing test reagents for mutagenicity tests, Ames tests, and chromosome aberration tests!

The "Kamada Brand Mutagenicity Test Rat Liver Homogenate Mutagenicity Test" is a test to investigate whether a certain substance (such as food, pharmaceuticals, food additives, paper, containers, exhaust gas, etc.) has mutagenic properties. "S-9" is standardized using liver prepared from male SD rats aged 7 weeks that have been administered phenobarbital and 5.6-benzoflavone as enzyme inducers via intraperitoneal injection. This product is highly regarded for its high enzyme activity and uniform quality. 【Features】 ■ High-activity enzyme system ■ Stored at -80℃ ■ Packaging unit: 1.5mL x 12 vials *For more details, please refer to the PDF document or feel free to contact us.

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Standard solution and reagents

Standard solution and reagents

We provide standard solutions and test samples! Introducing our product lineup.

We handle "standard solutions and reagents" at our company. Our lineup includes general-purpose chromatographic components such as "ULTRA standard substances," as well as "ICP-MS" and "ICP-OES" solutions, and "organic metal and oil" standard solutions for metals in hydrocarbon oils. Additionally, we also offer "standard solutions for atomic absorption" and "petroleum and biodiesel" standard solutions for metal and sulfur analysis in biodiesel. 【Partial Lineup】 ■ ULTRA standard substances ■ For ICP-MS ■ For ICP-OES ■ For atomic absorption (AA) ■ For petroleum and biodiesel ■ For organic metals and oil *For more details, please refer to the PDF materials or feel free to contact us.

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Derivatization for GC/MS analysis

Derivatization for GC/MS analysis

Proposals tailored to the purpose of analysis and the target object! GC/MS analysis of derivatized samples.

This document introduces a pretreatment method to enable the analysis of substances that are difficult to analyze using GC/MS. The pretreatment that converts difficult-to-analyze substances into analyzable substances is called "derivatization." Methods such as esterification, acylation, and silylation are used selectively according to the type and characteristics of the difficult-to-analyze substances. GC/MS measurements were conducted on citric acid solutions and citric acid solutions after esterification. It was found that citric acid, being a carboxylic acid, could not be effectively detected without derivatization, but after esterification, trimethyl citrate was detected, confirming the presence of citric acid. By performing appropriate derivatization, more accurate analysis becomes possible. [Analyzable Substances] ■ High volatility ■ High thermal stability ■ Low polarity *For more details, please refer to the PDF document or feel free to contact us.

  • Analytical Equipment and Devices
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Protein solubilization reagent MPEX PTS Reagents

Protein solubilization reagent MPEX PTS Reagents

Optimal solubilization reagents for analyzing membrane proteins using mass spectrometry (MS).

This is a solubilization reagent kit designed for the easy analysis of membrane proteins using mass spectrometry. Membrane proteins are said to be targets for many pharmaceuticals and are expected to be one of the protein groups that will see increasing research in the future. However, in analyses using mass spectrometry, it has been technically and temporally challenging to easily analyze them due to issues such as the removal of solubilizing agents and the inactivation of digestive enzymes. By using this product, which has a high solubilization capacity, membrane proteins can be easily solubilized without the need to consider different types of solubilizing agents. Even difficult-to-solubilize membrane-spanning proteins can be easily solubilized, and trypsin digestion can be performed in the presence of MPEX reagents. MPEX reagents can remove surfactants based on the principle of liquid-liquid extraction. It is possible to extract membrane proteins from membrane fractions of cell lysates, such as those from cultured cells, and analyze them using mass spectrometry. Features: - Higher solubilization capacity compared to Triton and Tween. - Does not inhibit the activity of digestive enzymes such as trypsin. - Easy removal method (liquid-liquid extraction).

  • Protein Extraction Kit
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Handling 400 types of valuable reagents! List of reagents handled by Nagara Science.

Handling 400 types of valuable reagents! List of reagents handled by Nagara Science.

We also produce custom reagents through contract purification and contract synthesis, so please make use of our services.

This catalog contains a list of reagents sold by Nagara Science Co., Ltd. We offer a variety of reagents related to "food and health food," including soybean isoflavones, tea catechins, strychnine, and tea methylated catechins, as well as "traditional Chinese medicine-related reagents" such as turmeric, curcumin, and turmeric bisacron. Additionally, we also handle "ceramide-related reagents" and "sialic acid glycan synthesis reagents." 【Reagent Products】 ■ Food and Health Food Related Reagents ■ Traditional Chinese Medicine Related Reagents ■ Ceramide Related Reagents ■ Sialic Acid Glycan Synthesis Reagents *This catalog is available in both English and Japanese. *For more details, please refer to the catalog or feel free to contact us.

  • Pharmaceutical materials and carriers
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Nagara Science Co., Ltd. Business Information

Nagara Science Co., Ltd. Business Information

We aim to commercialize university research outcomes to contribute to society and revitalize the university.

Nagara Science Co., Ltd. is a company that primarily manufactures and sells high-purity reagents by precisely separating approximately 400 types of components using its unique high-resolution HPLC columns. We can easily produce large quantities of reagents, and we have a rich inventory of reagents. Even if a reagent is out of stock, we can quickly mass-produce it. For large orders, we can offer very competitive prices, and we may also be able to provide crude purified products at special prices, so please feel free to consult with us. Additionally, we sell derivatives of sialic acid, glucose, galactose, lactose, and sialic acid-containing oligosaccharides. 【Business Activities】 ■ Manufacturing and selling high-purity reagents ■ Selling various derivatives and sialic acid-containing oligosaccharides ■ Contract services (purification, synthesis, analysis, instrumental analysis) ■ Development of high-resolution HPLC packing materials and fine particle classification technology *For more details, please refer to our catalog or feel free to contact us.

  • Other pharmaceutical intermediates and cosmetic ingredients
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Excerpt from the handbook: Reduction reactions using organosilicon compounds.

Excerpt from the handbook: Reduction reactions using organosilicon compounds.

It is possible to reduce compounds such as aldehydes, ketones, and acetals.

In the "Reduction Reactions Using Organosilicon Compounds," compounds such as "ketals," "esters," and "lactones" can be reduced. 【Features】 ○ SILICON-BASED REDUCING AGENTS ○ General Considerations ○ Silane Reduction of Alcohols to Alkanes For more details, please contact us or download the catalog. *This catalog is a digest version. If you need the full version, please contact us.

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New fixing solution "Artefix" that does not contain formaldehyde.

New fixing solution "Artefix" that does not contain formaldehyde.

Non-toxic and not classified as hazardous! Free from irritating odors and tear-inducing effects!

- Lower toxicity compared to formalin. - The mechanism of protein fixation is similar to that of formalin. - Using it as a storage solution after formalin fixation reduces exposure. - The use of formalin can be limited to specific areas. *For more details, please contact us at the URL below (or visit the website of Falma Co., Ltd.). Sample provision is also available. http://www.falma.co.jp/07question/index.html

  • Other experimental consumables
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[Sample available] Nucleic acid extraction reagent / CellEase II series

[Sample available] Nucleic acid extraction reagent / CellEase II series

[Sample available] No purification process required! DNA samples can be prepared in just 9 minutes.

The CellEase(R) II series nucleic acid extraction reagent is developed for gene extraction (DNA extraction) from cells, eliminating the need for complicated purification processes and allowing for the preparation of DNA samples that can be directly added to the gene amplification reaction (PCR) in just 9 minutes. CellEase II also eliminates the dilution step that was necessary with the conventional CellEase, achieving DNA extraction completion in a single tube. Additionally, the DNA extraction efficiency has significantly improved. 【Features】 ○ Only reagent addition and incubation required ○ No purification steps needed ○ Prepares genomic DNA for PCR in 9 minutes ○ Easy to automate ○ Significantly improved DNA extraction efficiency For more details, please contact us.

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[Sample available] Nucleic acid extraction reagent / CellEase Plant

[Sample available] Nucleic acid extraction reagent / CellEase Plant

[Sample available] It is possible to extract genes from plants and prepare DNA samples in just 9 minutes.

The CellEase(R) Plant nucleic acid extraction reagent for plants enables gene extraction from plants, which was difficult with the conventional CellEase and CellEase II. The protocol is the same as that of the conventional CellEase, and there is no need for complicated purification and separation processes. As a result, DNA samples that can be directly added to the gene amplification reaction (PCR) can be prepared in just 9 minutes. 【Features】 ○ For plants ○ Only 9 minutes ○ No purification required ○ Only temperature control Please contact us for more details.

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[Sample available] Nucleic acid extraction reagent / CellEase Blood

[Sample available] Nucleic acid extraction reagent / CellEase Blood

[Sample available] Gene extraction from whole blood is possible, and DNA samples can be prepared in just 9 minutes.

The CellEase(R) Blood nucleic acid extraction reagent for whole blood enables gene extraction from whole blood, which was difficult with the conventional CellEase and CellEase II. The protocol is the same as that of the conventional CellEase, eliminating the need for complicated purification and separation processes. As a result, DNA samples that can be directly added to the gene amplification reaction (PCR) can be prepared in just 9 minutes. 【Features】 ○ For whole blood ○ Only 9 minutes ○ No purification required ○ Only temperature control Please contact us for more details.

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Introduction to Custom Monoclonal Antibody Production Service Case Studies Vol. 8

Introduction to Custom Monoclonal Antibody Production Service Case Studies Vol. 8

An article introducing the selection of antibodies that distinguish specific proteins within family proteins.

Antibody artisans produce antibodies entirely in vitro without using animal immunization, allowing for detailed antigen design, such as narrowing down sequences and confirming cross-reactivity with different antigens during selection. In this case, we will introduce the antigen setup and selection method for producing antibodies that belong to a family of target proteins, binding only to the desired protein and not to other proteins within the same family. 【Contents】 ■1. Specificity of antibodies ■2. Preparation of antigens 　・2-1. Design of antigens 　・2-2. Production of conjugates ■3. Methods of selection 　・3-1. Panning 　・3-2. Screening ELISA ■4. Key points of selection 　・4-1. Design of antigens 　・4-2. Enhancing antibody specificity ■5. Summary *For detailed content of the article, please refer to the related links. *For detailed specifications, please refer to the PDF materials or feel free to contact us.

  • Other services
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  • Contract manufacturing
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Introduction to Custom Monoclonal Antibody Production Service Case Studies vol. 10

Introduction to Custom Monoclonal Antibody Production Service Case Studies vol. 10

It is possible to design fine antigens! An article introducing an example of antibody production that distinguishes a single amino acid substitution.

Antibody artisans perform selection in vitro against fixed antigens without using animal immunity. As a result, it is possible to conduct binding reactions in the state or environment where peptides or proteins are prepared, allowing for detailed antigen design, such as narrowing down the area of interest. Here, we will introduce an example of producing an antibody that can distinguish a substitution of one amino acid within a specific sequence. We encourage you to read on. [Contents] 1. Preparation of Antigens - 1-1. Narrowing down the target area - 1-2. Setting up antigens including negative controls - 1-3. Synthesizing peptides 2. Selection to Increase Specificity - 2-1. Excluding antibodies that bind to ends, etc. - 2-2. Increasing selection pressure with multiple antigens 3. Selection of Antibodies - 3-1. Checking the signal-to-noise ratio with ELISA - 3-2. Confirming the nucleotide sequence of the antibody 4. Summary *For detailed content of the article, please refer to the related links. *For detailed specifications, please refer to the PDF materials or feel free to contact us.

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  • Contract manufacturing
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Service Introduction: "Custom Oligonucleotide Synthesis Service"

Service Introduction: "Custom Oligonucleotide Synthesis Service"

Total support from basic research to clinical development! We provide oligonucleotides with optimal specifications.

At Ajinomoto Bio-Pharma Services Gene Design, we provide a variety of nucleic acid drug seeds such as siRNA, aptamers, antisense, decoys, miRNA, and CpG-ODN, tailored to optimal specifications for cell assays, in vivo experiments, in vitro diagnostic pharmaceuticals, and clinical trial GMP-compliant products. We also offer fluorescent labeling, such as Alexa modifications, and detection probes like LNA oligos at reasonable prices to support your basic research. 【Features】 - Extensive experience with cross-linked nucleic acids such as LNA and BNA, various modifications like 2’-OMe RNA and 2’-F RNA, and peptide, PEG, and ligand terminal additions. - Process development and analytical method development with an eye toward scale-up. - Experience in examining and validating standard testing methods. - Conducting stability tests necessary for GLP studies and clinical development. For more details, please contact us or download the catalog.

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  • Contract manufacturing
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Announcement of the Establishment of the Nucleic Acid Medicine API Development Center

Announcement of the Establishment of the Nucleic Acid Medicine API Development Center

[Announcement of New Base Establishment] Opening of a new GMP manufacturing facility for oligonucleotides.

Ajinomoto Bio-Pharma Services and Gene Design will strengthen its contract development and production system from the early stages of development to late stages and market launch by introducing large-scale production facilities. With the establishment of the new site, the production volume per lot will expand to the kilogram level. By combining our production capacity with the mass production technology of the Ajinomoto Group, a flexible and seamless supply system will be established, transitioning from conventional μg manufacturing to over 100 kg. [Overview] - Newly established within the research facility in Ibaraki City, Osaka Prefecture - Combined with the mass production technology of the Ajinomoto Group (AJIPHASE technology) - Establishing a supply system from conventional μg to over 100 kg *For more details, please refer to the PDF document or feel free to contact us.

  • Other contract services
  • Contract manufacturing
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miRNA mimic / S-TuD

miRNA mimic / S-TuD

We offer double-stranded RNA developed based on the results obtained from collaborative research!

We offer double-stranded RNA "miRNA mimic" with chemical modifications on the passenger strand and double-stranded RNA "S-TuD" primarily composed of 2’-O-Methyl RNA. The "miRNA mimic" allows you to observe the activity of the mature miRNA itself by inactivating the passenger strand. The "S-TuD" specifically binds to the Micro RNA Binding Site (MBS) of the RISC complexed mature miRNA and strongly inhibits miRNA activity even at low concentrations. 【Features】 ■ miRNA mimic - Double-stranded RNA with chemical modifications on the passenger strand - Enables observation of the activity of the mature miRNA itself - Product developed based on results obtained from collaborative research ■ S-TuD - Double-stranded RNA primarily composed of 2’-O-Methyl RNA - Strongly inhibits miRNA activity even at low concentrations - Negative control sequences are also available *For more details, please refer to the external link page or feel free to contact us.

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[Case Study] On the Use of PNA in Amplicon Sample Preparation

[Case Study] On the Use of PNA in Amplicon Sample Preparation

It is possible to estimate higher resolution feed organisms! Introduction of next-generation sequencing analysis examples *Analysis case collection is being distributed.

In our company, when preparing amplicon samples aimed at analyzing the microbial community structure present in plant leaves, we add PNA to suppress amplification from plant mitochondrial and chloroplast origins, resulting in favorable outcomes. Without the addition of PNA, approximately 90% of the acquired data consists of amplification products derived from the host. By adding PNA, we can reduce this to about 10%, allowing for a higher resolution estimation of the target organisms. [Results of condition testing with 4 sample units] ■ Ct1-Ct4: No PNA added ■ Mt1-Mt4: PNA added for mitochondrial blocking ■ Pl1-Pl4: PNA added for chloroplast blocking ■ PM1-PM4: PNA added for both mitochondrial and chloroplast blocking *For more details, please refer to the PDF document or feel free to contact us.

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[Next-Generation Sequencing Analysis Case] Analysis of Live Bacterial Microbial Community Structure

[Next-Generation Sequencing Analysis Case] Analysis of Live Bacterial Microbial Community Structure

Introducing a case that demonstrates the method using EMA can analyze live microbial communities! *Case study collection available.

We will introduce community structure analysis using the selective membrane permeable dye EMA (in-house data). For environmental samples dominated by E. coli, we prepared samples that were heat-treated and sterilized, as well as untreated samples, and performed EMA treatment. Subsequently, DNA extraction was conducted for each sample, followed by 16S rRNA gene amplicon analysis. As a result, in the community structure analysis, E. coli, which had become dominant, was hardly detected in the heat-treated samples due to its death. Additionally, in the related links, we also introduce community structure analysis using reverse transcription cDNA from RNA (in-house data), so please take a look. 【Workflow】 ■ Prepare environmental samples (heat-treated, untreated) ■ EMA treatment ■ Light irradiation ■ DNA extraction ■ Creation of adapter library via EMA-PCR *For more details, please refer to the PDF document or feel free to contact us.

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