Introduction to Custom Monoclonal Antibody Production Service Case Studies vol. 10

It is possible to design fine antigens! An article introducing an example of antibody production that distinguishes a single amino acid substitution.

Antibody artisans perform selection in vitro against fixed antigens without using animal immunity. As a result, it is possible to conduct binding reactions in the state or environment where peptides or proteins are prepared, allowing for detailed antigen design, such as narrowing down the area of interest. Here, we will introduce an example of producing an antibody that can distinguish a substitution of one amino acid within a specific sequence. We encourage you to read on. [Contents] 1. Preparation of Antigens - 1-1. Narrowing down the target area - 1-2. Setting up antigens including negative controls - 1-3. Synthesizing peptides 2. Selection to Increase Specificity - 2-1. Excluding antibodies that bind to ends, etc. - 2-2. Increasing selection pressure with multiple antigens 3. Selection of Antibodies - 3-1. Checking the signal-to-noise ratio with ELISA - 3-2. Confirming the nucleotide sequence of the antibody 4. Summary *For detailed content of the article, please refer to the related links. *For detailed specifications, please refer to the PDF materials or feel free to contact us.

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