Introduction to Custom Monoclonal Antibody Production Service Case Studies vol. 9

Not mediated by animal immunity! An article introducing successful cases of antibodies that distinguish specific oxidized Cys.

DJ-1 is a 21 kDa protein, and we present a successful case of producing antibodies that specifically recognize the oxidized form of the Cys residue at position 106 (C106) of DJ-1. We have attempted to obtain antibodies that specifically recognize the oxidized C106 through the production of polyclonal antibodies via immunization in rabbits and monoclonal antibodies via immunization in mice, but we were unable to obtain the desired antibodies in either case. Here, we introduce a method for producing antibodies that do not involve animal immunization and are selected entirely in vitro, along with the results of verifying the specificity of the obtained antibodies. 【Contents】 ■1. What is DJ-1 ■2. Strategic Screening 　・2-1. Antigen Design 　・2-2. Panning and ELISA Screening 　・2-3. Cross-reactivity Confirmation by ELISA ■3. Western Blotting and Immunoprecipitation ■4. Summary *For detailed content of the article, please refer to the related links. *For detailed specifications, please refer to the PDF document or feel free to contact us.

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