What are peak identification and quantification methods in HPLC? Free explanatory materials are currently available.
What are the differences and characteristics of the absolute calibration method and the internal standard method? A thorough and easy-to-understand explanation of each difference! Free basic knowledge materials are currently available.
Peak identification in HPLC is performed based on the retention time of peaks from standard samples. When information such as the UV-Vis spectrum from a DAD is simultaneously obtained, identification can also be done by considering the spectral matching. Quantification of the analyte is carried out by preparing a concentration series of standard sample solutions, determining the peak area or peak height from the chromatogram, and creating a calibration curve. This document provides an easy-to-understand explanation of peak identification and quantification in HPLC using diagrams, and also introduces absolute calibration curves and internal standard methods for quantification. We encourage you to read it. [Contents (partial)] ■ Introduction ■ HPLC Equipment ■ Separation in HPLC ■ Detection ■ Peak Identification and Quantification in HPLC *For more details, please download the PDF or feel free to contact us.
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