日本クレア List of Products and Services

In 2016, the Ministry of Education, Culture, Sports, Science and Technology announced in the Basic and Cross-Sectional Research Strategy Working Group that it would "support and develop technology for the establishment of an aging and longevity research environment that conforms to international standards" in the future direction of aging research. In response to the demand for the item "creation, breeding, and supply of aging animals and aging model animals," Japan CREA began securing inventory for the supply of high-aged mice every six months starting in 2018. Subsequently, for general sales, we are also securing the same process to provide approximately 100 mice each of ~78-week-old B6J/B6N (♂♀). Therefore, as long as inventory is available, we can supply high-aged mice "immediately" following the same procedures as general sales animals.

• Chemicals

A test system has been developed by Physiogenex using a unique method (using cyclodextrin, as described later) that allows for the extremely rapid induction of NASH within just three weeks. This model uses 8-week-old male mice and measures parameters such as ALT, AST, liver weight as a percentage of body weight, total cholesterol, triglycerides, and fatty acids, all of which increase within just three weeks (at 11 weeks of age). It also shows an increase in the NAS score (indicators: steatosis, inflammation, fibrosis, ballooning, total score) and significant liver inflammation and fibrosis. Treatment with ezetimibe (5 mg/kg) for two weeks results in a decrease in plasma total cholesterol and liver fat, a reduction in gene expression related to inflammation and fibrosis, and a lower overall NAFLD score due to decreased liver inflammation. Similarly, treatment with liraglutide for two weeks also shows a reduction in body weight and liver weight, as well as a lower overall NAS score due to decreased liver inflammation. For more details, please contact us. *Inquiries can also be made through our website. We will respond promptly. (Search for "Clea Japan" at http://www.clea-japan.com/)

• Other Contract Life Science Specialization

Unlike mice and rats, hamsters exhibit human-like cholesterol and bile acid metabolism characteristics. Therefore, pharmacological tests with the FXR agonist obeticholic acid show human-like weight and liver inflammation suppression, as well as side effects such as increased LDL cholesterol and decreased HDL cholesterol. (In mice and rats, this cholesterol side effect is reversed, with decreased LDL cholesterol and increased HDL cholesterol.) Physiogenex has adopted a cafeteria diet approach to induce this hamster model for overfeeding and NASH (non-alcoholic steatohepatitis) by providing a high-fat diet and high fructose water. This induces obesity, insulin resistance, hypercholesterolemia, and elevated ALT levels. This test system allows for efficacy evaluation studies targeting obeticholic acid and Elafibranor (GFT505), based on human-like cholesterol and bile acid metabolism characteristics. For more details, please contact us. *Inquiries can also be made through our website. We will respond promptly. (Search for "Clea Japan" at http://www.clea-japan.com/)

• Other Contract Life Science Specialization

The Physiogenex independently developed diet-induced NASH mouse model involves feeding a high-fat, high-cholesterol diet, along with the addition of fructose to drinking water. This induces severe liver damage, obesity, and insulin resistance. As a result, liver fat and hepatocyte ballooning appear by the 16th week of feeding, and severe liver complications (inflammation and fibrosis) develop by 25 weeks of age. Additionally, the administration of the FXR agonist obeticholic acid, which is related to NASH, results in observed reductions in obesity, insulin resistance, fatty liver, and ballooning at both 16 and 25 weeks of age. This experimental system allows for the efficacy evaluation of drugs using obeticholic acid as a control, set against a background of obesity and insulin resistance that resembles the histopathological features of human NASH. Furthermore, there are efficacy evaluation data and academic papers (PDF) using Elafibranor (GFT505) as a control drug. For more details, please contact us. *Inquiries can also be made through our website. We will respond promptly. (Search for "Clea Japan" at http://www.clea-japan.com/)

• Other Contract Life Science Specialization

We are developing a pharmacological evaluation model for diabetic nephropathy using the type 2 diabetes model SDT fatty rat. Currently, it has been clarified that unilateral nephrectomy and salt loading in drinking water (for 10 weeks) lead to the manifestation of diabetic nephropathy at an early age of 17 weeks. After several trials, we are still continuing our investigations as of 2017. The main data published by June 2017 are as follows: - The SDT fatty rats subjected to unilateral nephrectomy and salt loading show a decrease in GFR to below 50% at 17 weeks of age, presenting significant renal impairment. - Administration of the SGLT2 inhibitor Dapagliflozin for 10 weeks demonstrates an inhibitory effect on the progression of renal complications in SDT fatty rats subjected to unilateral nephrectomy and 0.3% saline loading. *Inquiries can also be made through our company website. We will respond promptly. (Search for "Clea Japan" at http://www.clea-japan.com/)

• Other Contract Life Science Specialization

The Royal College of Surgeons (RCS) rat, a model for retinal pigment degeneration, is the first known model of hereditary retinal degeneration. It has been widely used in research on hereditary retinal dystrophies. Since April 2016, Japan Crea has started general sales. For more details, please check from [Download Catalog]. *You can easily make inquiries using a simpler email form than on our company website. (Search for "Japan Crea")

• Other bio-related products and services

At Clea Japan, Inc., we primarily engage in the production and sale of laboratory animals, the manufacturing and sale of laboratory animal feed, and the production and sale of laboratory animal housing equipment, tools, facilities, and devices. In our contract services, we create transgenic mice and rats, as well as knockout mice. 【Features】 ■ Production of transgenic mice ■ Production of transgenic rats ■ Production of knockout mice ■ A diverse lineup of models *For more details, please refer to our catalog or feel free to contact us.

• Other contract services
• Other contract manufacturing

At Claire Co., Ltd., we primarily engage in the production and sale of laboratory animals, the manufacturing and sale of laboratory animal feed, and the production and sale of laboratory animal housing equipment, tools, facilities, and devices. In our contract services, we carry out the collection, freezing, and storage of fertilized eggs from mice and rats. To save space and breeding costs, we recommend collecting and freezing fertilized eggs or sperm from mice and rats that are only being housed. [Features] - Collection of fertilized eggs is possible - Freezing of fertilized eggs is possible - Storage of fertilized eggs is possible - Space-saving - Cost-saving on breeding *For more details, please refer to our catalog or feel free to contact us.

• Other contract services

At Claire Co., Ltd., we mainly engage in the production and sale of laboratory animals, the manufacturing and sale of laboratory animal feed, and the production and sale of laboratory animal housing equipment, tools, facilities, and devices. In our contract services, we accept mice and rats whose microbiological cleanliness is unknown. This service can be used for pre-import quarantine of imported animals and for pre-introduction quarantine from other facilities. We conduct microbiological testing according to customer requests. 【Features】 - Targets mice and rats with unknown microbiological cleanliness - Can be used for pre-import quarantine of imported animals - Can be used for pre-introduction quarantine from other facilities - Microbiological testing conducted according to customer requests *For more details, please refer to our catalog or feel free to contact us.

• Other contract services
• Microbiology

At Claire Co., Ltd., we mainly engage in the production and sale of laboratory animals, the manufacturing and sale of laboratory animal feed, and the production and sale of laboratory animal breeding equipment, tools, facilities, and devices. In our contract services, we carry out the production of individuals from frozen fertilized eggs of mice and rats. By conducting this within our BS facility, we are capable of large-scale processing and can also accommodate regular production supply. 【Features】 - Capable of producing individuals from frozen fertilized eggs of mice and rats - Conducted within a BS facility - Capable of large-scale processing - Can accommodate regular production supply *For more details, please refer to our catalog or feel free to contact us.

• Other contract services

At Claire Co., Ltd., we maintain and produce valuable mice and rats on behalf of our customers. We select housing facilities such as SPF facilities (bacterial system breeding rooms) and vinyl isolators based on production scale and microbiological levels. We also respond to requests for backcrossing, speed congenics, and selective breeding of breeding animals. 【Features】 ■ Selection of SPF facilities and housing based on production scale and microbiological levels ■ Meeting requests for backcrossing and speed congenics ■ Selective breeding of breeding animals is possible ■ Maintenance and production of valuable mice and rats on behalf of our customers *For more details, please refer to our catalog or feel free to contact us.

• Other contract services

At Nippon Crea Co., Ltd., we will take care of your mice and rats and perform microbiological cleaning (SPF and Gf). Please provide males and females that are capable of breeding. You can choose the method of cleaning from "natural mating → cesarean section" or "fertilized egg transfer using reproductive engineering techniques, etc." Additionally, the microbiological test items to confirm the completion of cleaning are based on the same items as our sold animals, but if you have requests for additional items, please consult with us. [Features] ■ Additional test items for completion of cleaning can be added ■ Cleaning methods can be selected ■ Consultation on target animals is available ■ Reproductive engineering techniques such as in vitro fertilization and tubal transfer can be utilized as needed *For more details, please refer to the catalog or feel free to contact us.

• Other Nucleic Acid/DNA Research
• Gene expression analysis contract
• Other contract services

This catalog is an introduction to the contracted services of Japan Kurea Co., Ltd., which mainly engages in the production and sale of laboratory animals, the manufacturing and sale of laboratory animal feed, and the production and sale of laboratory animal breeding equipment, tools, facilities, and devices. It includes a variety of services we offer, such as microbiological cleaning, contracted breeding (SPF), and strain maintenance and production services. [Contents] ■ Announcement of complete AAALAC certification ■ Introduction to contracted services *For more details, please refer to the catalog or feel free to contact us.

• Other Nucleic Acid/DNA Research
• Gene expression analysis contract
• Other contract services