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This document is the instruction manual for "PXB-cells LA," fresh human liver cells isolated from PXB mice (for lipid research purposes). It includes key operations after receipt (operations at the time of receipt, maintenance culture, analysis culture), product features and specifications, and the equipment and reagents to be used. To maintain the lipid profile of this product and utilize it for research, please read this document carefully and use it correctly. 【Contents (partial)】 ■ Introduction ■ Features of PXB-cells LA ■ Product specifications ■ Equipment and reagents to be used ■ Handling of PXB-Cells LA *For more details, please refer to the PDF document or feel free to contact us.

• Human cells
• Other bio-related products and services

"PXB-cells LA" are human fatty liver cells optimized for lipid analysis, derived from fresh human liver cells (PXB-cells) collected through collagenase perfusion from human liver chimeric mice (PXB mice) using a special culture method. It has been confirmed that they can maintain the same lipid metabolic functions as fresh human liver cells at a high level for up to 10 days after delivery. Additionally, it is possible to maintain the secretion of human albumin, drug metabolism enzymes, and transporter activity for an extended period under culture conditions. 【Features】 ■ Maintains the same lipid metabolic functions as fresh human liver cells at a high level for up to 10 days after delivery ■ Forms large lipid droplets similar to the tissue morphology of human fatty liver within hepatocytes ■ Confirms the secretion of large VLDL (Large-VLDL) with a large particle size ■ Provides high-quality fresh human liver cells from the same donor at a low and stable supply ■ Confirms that similar responses to clinical trials can be obtained with anti-lipid drugs *For more details, please refer to the PDF document or feel free to contact us.

• Human cells
• Other bio-related products and services

The "PXB Mouse" is an experimental animal that has a human liver, making it closer to humans. As a result, it is possible to predict the efficacy and safety in humans with a higher probability than traditional animal testing in non-clinical trials for drug development. This can improve the success rate of new drug development and, as a result, reduce the time and cost involved in development. 【Features of the "PXB Mouse"】 ■ It has a high replacement rate of human liver. ■ It forms normal human liver tissue. ■ It expresses human-type genes, enzymes, and transporters in the liver. ■ It can sustain infection with hepatitis viruses (HBV, HCV) that only infect humans. *For more details, please refer to the PDF document or feel free to contact us.

• Other experimental consumables
• Other animal and plant experimental equipment and instruments
• Other experimental equipment and supplies

We provide fresh human liver cells, "PXB-cells," isolated from the "PXB mouse," a chimeric mouse with a human liver. The fresh human liver cells, "PXB-cells," can maintain high human metabolic enzyme activity over a long period and can be used for drug metabolism, enzyme induction/inhibition, safety assessments, transporter evaluations, and anti-HBV drug efficacy evaluations in cell experiments. Please feel free to contact us if you have any requests. 【Features of "PXB-cells"】 ■ Stable supply of liver cells from the same donor (repeated experiments possible) ■ Long-term culture and maintenance of high human-type activity (over 3 weeks) ■ High cell adhesion (flat and spheroid culture possible) ■ Persistent infection with hepatitis virus (HBV) ■ In vitro and in vivo experiments possible with liver cells from the same donor *For more details, please download the PDF or feel free to contact us.

• Human cells
• Other bio-related products and services