ジェイテックコーポレーション List of Products and Services

With about 30 minutes of preparation, you can leave the medium exchange work to the device, reducing the need for weekend work! 'MakCell' is an automatic cell culture device that automatically performs the tedious medium exchange on a daily basis. Since the medium exchange is done without removing the cells from the CO2 incubator, it minimizes the stress caused by vibrations from transportation and changes in humidity and temperature that occur during regular medium exchanges. Dishes and multi-well plates can be mixed for a total of up to 24 plates. At the top, there is a refrigerated section for supplying medium, allowing for the simultaneous loading of three types of media. ★ Currently, we are offering technical materials provided by university professors. You can view them immediately by clicking the download button below.

• Culture Equipment

A unique etching technology that utilizes catalytic action to chemically remove only the protrusions on the processed surface by moving ultra-pure water as the processing fluid over a substrate with pads that have catalytic functions (such as films of Pt or Ni). It flattens the surfaces of various materials, including glass and SiC, at the atomic scale. CARE further achieves a P-V of 0.7 nm and aims to become the ultimate processing method capable of achieving flatness at the level of a single atom in the future.

• Other processing machines

EEM (Elastic Emission Machining) processing is a unique surface nano-processing technology that utilizes chemical reactions between nanoparticles and the surface of the workpiece. By interacting reactive nanoparticles in ultra-pure water with the surface atoms of the workpiece, it removes them to achieve a perfectly flat surface at the atomic level.

• Surface treatment contract service

We will introduce the gas exchange capability of disposable culture vessels. Human iPS cells were able to form spheroids in both culture vessels, but the dissolved oxygen levels significantly decreased during culture in glass. Osteosarcoma cells could not be cultured in glass, but they were able to be cultured in disposable vessels. 【Analysis Method】 ■SevenExcellence S900; pH/DO (MetraTredo) *For more details, please refer to the PDF document or feel free to contact us.

• Other Culture
• Cell dispersion reagent
• Other cell research

We would like to introduce the media exchange for dimple plates using the tabletop automatic cell culture device "MakCell." The obtained cell aggregates showed a reduction in small sizes that appeared to be broken, and no large sizes that seemed to have fused were observed, suggesting that there was no collapse within the dimples or movement between the dimples. ▼ Do you have any of these concerns? MakCell can solve them! - Cells move or break during media exchange. - There are inconsistencies in cell sizes. - I want to culture cells of uniform size. - I want to automate media exchange. ~ Limited Information on the Tabletop Automatic Culture Device "MakCell" ~ Only 2 units available (as of March 2023), the regular price of 13,700,000 yen (excluding tax) will be offered at a discounted price of 9,800,000 yen (excluding tax) for deliveries by the end of June 2023, which is approximately a 28.5% discount! [Additional delivery adjustment fee: 300,000 yen (excluding tax)] *For more details, please refer to the PDF document or feel free to contact us.

• Other Culture

We will introduce a comparative evaluation between the tabletop automatic cell culture device "MakCell" and manual culture. Compared to manual culture, cell culture using MakCell showed an increase in the number of cells obtained. Regarding cell morphology, no significant changes were observed, and floating cells were confirmed in both MakCell and manual culture. ~Limited Information~ Only 2 units available in stock (as of March 2023), the list price of 13,700,000 yen (excluding tax) will be offered at a discounted price of 9,800,000 yen (excluding tax) for deliveries by the end of June 2023, which is approximately a 28.5% discount! [Delivery adjustment fee: 300,000 yen (excluding tax) separately] *For more details, please refer to the PDF document or feel free to contact us.

• Other Culture

This document contains technical information on "Fragmentation of F-PDOs / Evaluation of Anticancer Drugs" using the spheroid fragmentation and dispersion device "CellPet FT." It features photographs showing the condition after processing immediately, as well as at 3, 9, and 13 days of culture, using a 70μm mesh filter. Please feel free to contact us if you have any inquiries. *For more details, please refer to the PDF document or feel free to contact us.*

• Other Culture
• Cell dispersion reagent
• Other cell research

We will introduce the "CellPet 3D-iPS," a three-dimensional rotating floating culture device, regarding "differentiation induction." From the analysis results, we were able to demonstrate a method for inducing trilineage differentiation using the CELLFLOAT system. It is expected that a certain amount of growth factors and low molecular compounds will be necessary for preferential differentiation into each germ layer. However, the results suggest that it is possible to induce differentiation for each type. You can download technical materials from the related catalog below. Please take a look. [Analysis Method] ■ Spheroids were collected on day 7 of culture, observed and photographed, followed by RNA extraction. Real Time RT-PCR analysis was performed using QuantStudio7. * For more details, please refer to the PDF materials or feel free to contact us.

• Other Culture
• Cell dispersion reagent
• Other cell research

We would like to introduce the "CellPet 3D-iPS," a three-dimensional rotating floating culture device, regarding "induction of chondrogenic differentiation." The analysis results indicate that our product enables easy differentiation from hMSC to chondrocytes. We believe that the maturation of the formed cartilage tissue can be promoted through rotating floating culture in the CellPet CUBE, which allows for greater shear stress. You can download technical materials from the related catalog below. Please take a look. 【Analysis Method】 ■Alcian Blue staining/Kerner's solution *For more details, please refer to the PDF materials or feel free to contact us.

• Other Culture
• Cell dispersion reagent
• Other cell research

We would like to introduce the "CellPet 3D-iPS" three-dimensional rotating floating culture device for the culture of iPS cells. With the CELLFLOAT system, we were able to obtain spheroids that maintained their undifferentiated state. The size of the spheroids had a mode of 220-240 μm, with 61.9% (1865 out of 3013) existing within the range of 200-300 μm. You can download technical materials from the related catalog below. Please take a look. 【Overview】 ■ Cell type: 253G1 (Pre-culture: 253G1 cells cultured in Φ60 dishes coated with iMatrix511 (Nippi)) ■ Culture medium: AK02N (Ajinomoto) ■ Detachment solution: EDTA (5 mM) ■ Culture vessel: Disposable culture vessel <10ml> ■ Culture conditions (rotation speed): 40 rpm *For more details, please refer to the PDF materials or feel free to contact us.

• Other Culture
• Cell dispersion reagent
• Other cell research

Automatic completion of troublesome extraction tasks! Introducing the automatic magnetic bead nucleic acid extraction device priced at 1,650,000 yen (excluding tax). Simultaneous extraction of 32 samples is possible, and the shortest extraction time is just 15 minutes. Operation is easy! Settings can be completed using a touch panel monitor. Please feel free to contact us when you need assistance. 【Features】 ■ Simultaneous extraction of 32 samples ■ Dedicated kit for both DNA and RNA ■ Shortest extraction time of 15 minutes ■ Easy operation with a touch panel monitor ■ Up to 500 protocols can be registered ★ Please download the product information. ★ We also accept requests for demo units. Feel free to contact us.

• Refining and Extraction Equipment
• Other purification and extraction

"MakCell" is a device that performs automatic medium exchange within a CO2 incubator. The interior is equipped with a HEPA filter, maintaining cleanliness equivalent to ISO Class 5, thereby reducing the risk of contamination. When medium exchange is performed manually by removing samples from a standard incubator, changes in temperature, humidity, and external air (composition) are unavoidable, which can cause stress and damage to the cells. However, with "MakCell," medium exchange is executed without removing samples from the incubator, minimizing stress and damage to the cells. It is a "cell-friendly device" that you can trust for medium exchange! Additionally, it is a compact tabletop device, taking up little space and allowing operation at eye level. ~Limited Information~ Only 2 units available (as of March 2023), the regular price of 13,700,000 yen (excluding tax) will be offered at a discounted price of 9,800,000 yen (excluding tax) for deliveries by the end of June 2023, a discount of approximately 28.5%! [Delivery adjustment fee: 300,000 yen (excluding tax) separately] *For more details, please refer to the PDF document. Feel free to contact us with any inquiries.

• Culture Equipment

"MakCell" is a tabletop automatic cell culture device that allows for automatic medium exchange within a CO2 incubator. It reduces stress on cells caused by environmental changes such as CO2 concentration, temperature, and humidity, as well as damage to cells due to the movement (vibration) of culture containers during medium exchange. Designed with consideration for cross-contamination, it enables easy management of culture schedules through simple operations on a PC. 【Features】 - Automatic medium exchange within a CO2 incubator - Capable of simultaneously holding three types of media - Usable with both dishes and multi-well plates - Designed with consideration for cross-contamination - Easy management of culture schedules via PC ~Limited Information~ Only 2 units available in stock (as of March 2023), the regular price of 13,700,000 yen (excluding tax) will be offered at a discounted price of 9,800,000 yen (excluding tax) for deliveries by the end of June 2023, which is approximately a 28.5% discount! [Delivery adjustment fee: 300,000 yen (excluding tax) separately] *For more details, please refer to the PDF document or feel free to contact us.

• Culture Equipment

- Large-capacity incubator Adopts a rotating shelf to accommodate automation. Equipped with dry heat sterilization function. Can hold 84 multi-well plates or dishes. - Clean environment Equipped with a HEPA filter. The interior of the device is under the same conditions as a clean bench. - Cartesian robot Offers high degrees of freedom, ensuring fast and accurate movements. Easy maintenance. - Customization Automates your cell culture methods as they are.

• Culture Equipment
• CO2 Incubator

"MakCell" is a product that has improved upon our flagship product "CELLPET II" by enhancing the automatic cell culture technology to meet our customers' needs to the fullest. It is equipped with a HEPA filter and allows for medium exchange in an environment equivalent to Class 100. It can accommodate both multi-well plates and dishes for culture. 【Features】 ■ Equipped with HEPA filter ■ Medium exchange in an environment equivalent to Class 100 ■ Usable with both multi-well plates and dishes ■ Compact tabletop size ■ Supports three types of media (with cooling function) ■ Email notification function for device operation status (optional) ■ Compatible with low oxygen specifications (optional) ~Limited Information~ Only 2 units available in stock (as of March 2023), the regular price of 13,700,000 yen (excluding tax) will be offered at a discounted price of 9,800,000 yen (excluding tax) for deliveries by the end of June 2023, which is approximately a 28.5% discount! [Delivery adjustment fee: 300,000 yen (excluding tax) separately] *For more details, please refer to the PDF document or feel free to contact us.

• Culture Equipment

The "Tissue Dissociation Plate" is a trimming-specific plate with a concave bottom shape and a pointed grid surface, suitable as a substitute for petri dishes. This design reduces the burden of cutting as the tissue (tumor) does not slip. Additionally, it allows for the rapid and fine dispersion of minced tissue (tumor). It can be used for tissue dissociation in primary cultures, trimming of tissue pieces and pathological specimens, and as a dissection platform for mouse embryos. 【Features】 ■ Concave bottom shape and pointed grid surface ■ Reduces the burden of cutting as the tissue (tumor) does not slip ■ Allows for rapid and fine dispersion of minced tissue (tumor) ■ Wide groove structure on the outer perimeter ■ Convenient for recovering dispersed tissue (tumor) *For more details, please refer to the PDF document or feel free to contact us. If you need a sample, please do not hesitate to ask.

• Other glassware

We would like to introduce our product, "CellPet3D-iPS/CellPet FT." "CellPet3D-iPS" is a rotating floating culture device that enables cultivation in spheroids. It can be utilized in research fields such as regenerative medicine and drug discovery. "CellPet FT" is a cell fragmentation and dispersion device that automatically controls the flow rate, helping to suppress variations in fragmentation. It can be used in drug discovery research. 【Features of CellPet3D-iPS】 ■ Technology established uniquely by our company: "CELLFLOAT" ■ For the culture of iPS cells, the medium exchange frequency is once every 3 to 4 days ■ Enables cultivation in spheroids (organoids) ■ Reduces contamination ■ Uses a closed system culture container *For more details, please refer to the PDF document or feel free to contact us.

• Dispersion/emulsification equipment/homogenizer

The "Tissue Dissociation Plate" is a product designed for tissue (tumor) dissociation that reduces the burden of cutting work by preventing the tissue from slipping. We also offer the "CellPet FT," a device for cell fragmentation and dissociation that minimizes damage to cells during the dissociation process. 【Features】 ■ Concave bottom shape and pointed grid surface - Reduces the burden of cutting work by preventing the tissue (tumor) from slipping - Allows for quick and fine dissociation of chopped tissue (tumor) ■ Wide groove structure around the perimeter - Convenient for collecting the tissue (tumor) after dissociation - Minimizes loss during collection *For more details, please refer to the PDF document or feel free to contact us.

• Other Analysis
• Other analysis and evaluation services
• Other emulsification and dispersion equipment

The cell fragmentation and dispersion device fragments spheroids (cell aggregates) into a uniform size, and is utilized in drug discovery. It reduces damage to cells during dispersion and minimizes contamination risks. 【Features】 - Variation reduction / Automatically controls flow rate to suppress fragmentation variation - No reagents required / Simple fragmentation through physical action - Contamination reduction / Achieves fragmentation through operations in a closed system *For more details, please refer to the PDF document or feel free to contact us.

• Other Culture
• Cell dispersion reagent
• Other cell research

The "CellPet FT handy" is a lightweight dispersing device weighing 3.3 kg, making it easy to install in clean benches or safety cabinets. With simple operability, it dispenses 1/10 of the capacity with just one lever operation by tilting the side lever. Additionally, we also offer the "CellPet FT," a cell fragmentation dispersing device that evenly breaks up cell aggregates. 【Features of CellPet FT handy】 ■ Lightweight design ■ Easy installation in clean benches or safety cabinets ■ Simple operability ■ One lever operation by tilting the side lever ■ Compact size: height 38.3 cm, base dimensions 14 cm wide, 18.8 cm deep *For more details, please refer to the PDF materials or feel free to contact us. Demonstrations are also available upon request.

• Other emulsification and dispersion equipment

Features - The device is compact, so it does not take up much space even when placed inside an incubator. - The dedicated culture container rotates, allowing the medium to be dispersed and used effectively. - By placing cells that have been adhered and cultured on a dish/plate into the dedicated container for rotating suspension culture, spheroids can be easily formed. - It does not require scaffolding materials. - It can perform continuous culture from undifferentiated stem cells to differentiated cultures. - Three-dimensional culture generally results in increased gene expression compared to two-dimensional culture. - The material of the dedicated container has very high gas permeability, preventing oxygen deficiency even in a closed system. - The size of the gas-permeable resin container is 10ml (with 30ml and 50ml versions under development).

• Culture Equipment
• Other Physics and Chemistry Equipment

**Features** "Reduces damage to cells during dispersion" - Uses only a mesh filter without reagents like enzyme treatments. - Does not disperse to single cells, maintaining the connection between cells. - No repeated pipetting; the mesh is passed through only once. "Reduces contamination risk" - Achieves a closed environment during dispersion by using sealed containers. "High repeatability" - Can control the dispersion speed, eliminating variability caused by human factors. "Maintains original properties" - Research results indicate that dispersing patient-derived cancer cells with CellPet FT maintains clinical characteristics better than using enzymes.

• Cell dispersion reagent
• Other cell research
• Other Physics and Chemistry Equipment

Features - The device is compact, so it does not take up much space even when placed inside an incubator. - The dedicated culture container rotates, allowing the medium to be dispersed and used effectively. - By placing cells that have been adherently cultured on dishes/plates into the dedicated container for rotating suspension culture, spheroids can be easily formed. - It does not require scaffold materials. - It can perform continuous culture from undifferentiated to differentiated stem cells. - Three-dimensional culture generally increases gene expression levels compared to two-dimensional culture. - The material of the dedicated container has very high gas permeability, ensuring that there is no oxygen deficiency even in a closed system. - The size of the gas-permeable resin container is 10ml (with 30ml and 50ml sizes currently in development).

• Culture Equipment
• Other Physics and Chemistry Equipment

Features - The device is compact, so it does not take up much space even when placed inside an incubator. - Culture containers can be attached to both sides of the device, allowing for use in a maximum of two pairs. - By applying shear stress to the cells, differentiation is promoted compared to 2D cultures. - Three-dimensional culture increases gene expression levels compared to two-dimensional culture. - The rotation of the dedicated culture container allows for effective diffusion of the culture medium. - One side of the dedicated container has a high gas exchange membrane, enabling long-term culture. - The dedicated culture containers are available in 100ml and 50ml sizes. - With optional features, scaling up is possible (approximately 600ml).

• Culture Equipment
• Other Physics and Chemistry Equipment