Establishment of the UpCell User Club

It is possible to collect mesenchymal stem cells, dendritic cells, macrophages, and cell sheets without using trypsin and without causing damage.

- Celsid's Technology By utilizing a unique nano-surface design, temperature-responsive polymer (PIPAAm) has been immobilized on the surface of the equipment. This allows the surface to change from hydrophobic (cell-adhesive surface) to hydrophilic (cell-releasing surface) by lowering the surface temperature around 32°C. Without using any enzymes that can damage cells, such as trypsin, intact cells can be recovered simply by setting the equipment temperature to 20-25°C and waiting for about 10-30 minutes. - Benefits 1) Easy cell recovery through temperature control. 2) Recovered cells completely retain the extracellular matrix. 3) No need for trypsin, which can harm cells. 4) Suitable for culturing tissues used in regenerative medicine research. 5) For 3D culture by stacking homo/heterogeneous cell sheets. For more details, please contact us or refer to the catalog.

• Plastic utensils and containers
• others

Sample presentation available! Cell sheets can be easily collected and transplanted. We use materials that are gentle on cells.

Features Dedicated cell sheet recovery support "CellShifter" for UpCell Utilizes a material with flexibility and water absorption suitable for cell detachment that could not be achieved with hydrophilic PVDF membranes or nitrocellulose membranes. This allows for the reliable and easy recovery of your precious cell sheets that have been carefully cultured with UpCell. The CellShifter, with the cell sheet adhered to it, boasts excellent ease of handling when transplanted directly to the host. Thanks to the specially treated material, there are almost no detached fibers. We guarantee maximum experimental results. Please take this opportunity to experience the results of years of material development.

• others

No need for trypsin! Macrophages and others are collected intact. The temperature-responsive culture equipment UpCell has "grid walls" arranged at 3mm intervals.

◆The temperature-responsive culture device UpCell for cell recovery is equipped with "grid walls" placed at 3mm intervals. ◆It has become easier to recover cultured cells in single cell/small colony form. ◆The grid wall is a new technology that prevents cells from proliferating beyond the grid by using optimized grooves on the surface of the culture dish. Cells can be recovered intact with just a short time and temperature treatment. Advantages of RepCell - It is known that trypsin significantly damages cell viability, but with RepCell, it is possible to recover cells while maintaining surface antigens and extracellular matrix. - Cell detachment using only temperature treatment The only necessary procedure for cell recovery is temperature treatment, eliminating the need for cumbersome pipetting operations associated with traditional trypsin use. For more details, please contact us or refer to the catalog.

• others

Complete suppression of cell adhesion in cell culture.

By applying our unique nano-surface design technology, we have fixed (covalently bonded) superhydrophilic polymers to the surfaces of our equipment. In addition to being superhydrophilic, this polymer creates a free water film. The double effect of "superhydrophilicity" and "free water" has achieved a level of low adhesion performance that far surpasses conventional low-adhesion equipment. For more details, please contact us or refer to our catalog.

• others

Live demonstration available! While culturing cells, we will measure the TER (transepithelial/endothelial resistance) and Ccl (capacitance) of the cell layer in real-time.

Features 1) Measurement possible while culturing cells: Long-term measurements can be conducted with the cell module placed inside the incubator. 2) Medium exchange and addition of reagents possible: The module can be removed from the incubator for operations. 3) Compatible with cell culture inserts from various manufacturers: Provides optimized medium volume for each company's inserts. 4) Each electrode is independent: Measurement of up to 24 samples is possible. (+/2) Cleaning the electrodes is easy, and inserts of different sizes can also be used. Specification of the electrodes to be measured is possible. Demonstrations are available. For more details, please contact us or refer to the catalog. You can also watch an introduction video of cellZscope on our YouTube channel.

• others

At our cell culture center, we conduct contract services related to cell sheet manufacturing, facility operation, and the preparation of application documents.

Features of Regenerative Medicine Contract Services 1. Development and Contract Manufacturing of Cell Sheet Products We provide contract development and manufacturing services primarily for cell sheets, commissioned by pharmaceutical companies and research institutions. Our team includes clinical culture specialists certified by the Japanese Society for Regenerative Medicine, and we offer high-quality cell sheet product development and manufacturing using experienced staff in a cell culture processing facility licensed for the production of specific cell processed products. 2. Facility Management and Application Support The "Act on Securing Safety for Regenerative Medicine" stipulates the structure, equipment, and operation of cell processing, requiring each cell culture processing facility to submit notifications and applications for permits to the Minister of Health, Labour and Welfare. At CellSeed, we assist with the notification and application documentation for the manufacture of specific cell processed products, as well as the creation of necessary operational documents. Additionally, we support the necessary administrative procedures, including the preparation of consultation materials for authorities regarding applications such as clinical trial notifications. 3. Education for Cell Culture Technicians We conduct training for cell culture technicians who have no prior experience with cell sheets, covering procedures such as cell sheet culture and detachment at our cell culture facility. For more details, please contact us or refer to our catalog.

• Other contract services

Live demonstration available! The difference compared to metal heating plates is clear!

It is a transparent heating element made of specially processed hard glass. It improves work efficiency in clean benches for tasks such as microscope observation and media exchange using UpCell/RepCell. Temperature settings are possible from room temperature to 50°C. It is lightweight (approximately 70g) and suitable for portability. It can also be used to cover petri dishes and multi-well plates to prevent fogging during photography. Demonstrations are available. For more details, please contact us or refer to the catalog.

• others

UpCell dedicated cell sheet stacking device. Stacking of sheets becomes easy. Demo unit available.

Features of CellStamp ● Utilizes the adhesion of gelatin gel and cell sheets for layering * ● Can be sterilized by autoclaving ** ● Compatible with UpCell 3.5 cm dishes and 24-well multi-wells

• Other physicochemical equipment

Technoa Co., Ltd. (Headquarters: Gifu City, Gifu Prefecture, Representative Director: Koji Yamazaki) will hold a limited-time summer special event, "Monozukuri DX CAMP," from Saturday, August 9, 2025, to Saturday, August 23, 2025. Small and medium-sized manufacturing companies face challenges such as a shortage of specialized personnel and a lack of know-how and information, which act as barriers to promoting DX (digital transformation). This event will provide a step-by-step approach to "realistic DX," based on the know-how accumulated by Technoa while supporting the DX transformation of small and medium-sized manufacturing companies. It will be structured as DAY.1 to DAY.5, offering an opportunity for skill development during the summer break and supporting the learning and growth of small and medium-sized manufacturers. For more details, please refer to the related link.

JOHNAN has updated a webpage summarizing the selection points to solve various challenges in large-scale processing (quality maintenance, short delivery times, costs). This article introduces the challenges faced in large-scale processing based on years of knowledge and experience, as well as the key points for choosing the optimal contractor that balances quality and short delivery times. If you are looking for a reliable contractor, please make use of our "Large-Scale Processing Solutions." ▼For more details "Large-Scale Processing Solutions to Achieve Product Quality and Short Delivery Times" https://www.johnan.com/solution/process/processing-assembly/post_8.html

Thank you very much for your continued support. We sincerely apologize for the inconvenience, but our company will be closed for summer vacation during the following period. 【Closure Period】 From Saturday, August 9, 2025, to Thursday, August 14, 2025. We will resume normal business operations on Friday, August 15. We appreciate your understanding during this time and apologize for any inconvenience caused.

JOHNAN has released a video that clearly explains the implementation technology for stretchable films, which are expected to be utilized in wearable devices and medical equipment. In the video, you can see how the film, which has excellent elasticity, stretches and contracts. Please check our high technological capabilities and the flexible characteristics that are useful for product development. We hope this will help you generate new ideas in product development. ▼You can watch the video here [Link to the implementation page for stretchable films] https://www.johnan.com/dms/mounting/filmdevic/stretchable/

"Because it's an invisible place, we won't overlook it." Japan's climate is characterized by high temperatures and humidity, and particularly after typhoons and heavy rains, the conditions can be harsh for homes. It's not just the roofs and exterior walls that are affected by rain and wind. The area beneath the floor is actually the most susceptible and often overlooked point. This time, we will explain the "checkpoints for under the floor" that should be confirmed after a typhoon or heavy rain, the "responses" if abnormalities are found, and measures for preventing recurrence. [Table of Contents] ■ Why is underfloor checking necessary? ■ Checkpoints to confirm after a typhoon or heavy rain ■ Responses when damage is discovered ■ Points for preventing recurrence ■ Suggestions from Seihou Products ■ Summary Please be sure to read the [related materials] and [related links]. ↓ ↓ ↓