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  1. Home
  2. Testing, Analysis and Measurement
  3. ティー・エイ・インスツルメント・ジャパン (TAInstruments)
  4. July Online LIVE Seminar! ~Protein Analysis Seminar~
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  • Jun 08, 2022
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Jun 08, 2022

July Online LIVE Seminar! ~Protein Analysis Seminar~

ティー・エイ・インスツルメント・ジャパン ティー・エイ・インスツルメント・ジャパン (TAInstruments)
13:00~14:00 Analysis of the interaction between sleep substance-synthesizing enzyme and ligand using isothermal titration calorimetry Shigeru Shimamoto, Lecturer, Department of Life Science, College of Science and Engineering, Kinki University Isothermal titration calorimetry (ITC) has the significant advantage of detecting molecular interactions in a form that is closer to the state present in vivo, without the need for chemical modifications, immobilization, or detection probes. Furthermore, it is a method that provides nearly complete thermodynamic parameters related to the interactions. Here, I will introduce the analysis of the interaction between lipocalin-type prostaglandin D synthase (L-PGDS), which acts as a sleep substance-synthesizing enzyme in the mammalian brain, and its ligand, prostaglandin (PG), using ITC, and discuss the elucidation of the enzyme reaction mechanism in conjunction with structural information. 14:00~14:30 Introduction to isothermal titration calorimetry (ITC) and differential scanning calorimetry (DSC) for biomacromolecules Minako Maeda, Applications Department, TA Instruments
Date and time Friday, Jul 22, 2022
01:00 PM ~ 02:30 PM
Please apply through the link.
Capital
Entry fee Free Thank you very much for your participation.
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Related product

Discovery DSC.jpg

Differential Scanning Calorimeter Discovery DSC (Calorimeter)

How about one for the lab? Introducing the Discovery DSC series, the world's most outstanding and highly versatile differential scanning calorimeter.

TA Instruments continues to be the leading company in the world of DSC with innovative and outstanding technology. It provides reliable data and enhances workflow and productivity in laboratories. Material property analysis is essential at all stages of product design, manufacturing, processing, and application. Differential Scanning Calorimetry (DSC) is the most widely used thermal analysis instrument for evaluating the physical properties of a wide range of materials, including polymers, pharmaceuticals, food, biomaterials, organic compounds, inorganic compounds, and composites.

  • Analytical Equipment and Devices

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Collection of Thermal Analysis Case Studies for Protein Analysis (Calorimeter) ITC DSC

This is an introduction to protein analysis cases and related equipment by analysis specialists. This time, we will cover three devices: ITC, TAM, and NanoDSC!

Measurement capabilities offered by TA Instruments ☆ Thermal analysis / glass transition, crystallization, thermogravimetric analysis, curing reactions, expansion rate, thermal conductivity, shrinkage rate, effects of moisture, etc. ☆ Viscoelastic measurements (rheology, rheometers) / viscoelasticity, elastic modulus, viscosity, stress relaxation, creep, master curves, residual strain, molecular weight, molecular weight distribution, etc. ☆ Microcalorimetry / intermolecular interactions, crystallization, amorphousness, aggregation/precipitation, dispersibility, solubility, compatibility, wettability, stability, adsorption, etc. ☆ Fatigue testing / tensile strength, temperature dependence, viscoelasticity, elastic modulus, viscosity, stress relaxation, creep, master curves, residual strain, etc.

  • Analytical Equipment and Devices

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1-DSC250_MicroCamera-Detail_jpg.jpg

Accessory for Differential Scanning Calorimeter (DSC) - Microscope Camera

Device accessories

The DSC measurements conducted with microscope accessories provide insights through multiple applications to better understand the properties and behaviors of materials. It is possible to capture images related to crystallization and melting transitions, flow of materials above the glass transition and melting points, phase transitions, and volume dimensional changes associated with evaporation and sublimation. Visual observation of color changes and other appearance alterations is used in combination with DSC data to gain a deeper understanding of the material being analyzed.

  • Color camera

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イプロス_サムネイル(タンパク質分析_ITC).jpg

Analysis case: Evaluation of substrate decomposition rate and inhibitor efficacy by ITC.

This is an introduction to a protein analysis case study by a specialist in analysis. This time, we evaluated the substrate degradation rate and the effects of inhibitors using ITC.

Measurement capabilities offered by TA Instruments ☆ Thermal analysis / glass transition, crystallization, thermogravimetric analysis, curing reactions, expansion rate, thermal conductivity, shrinkage rate, effects of moisture, etc. ☆ Viscoelastic measurement (rheology) / viscoelasticity, modulus of elasticity, viscosity, stress relaxation, creep, master curve, residual strain, molecular weight, molecular weight distribution, etc. ☆ Microcalorimetry / intermolecular interactions, crystallization, amorphousness, aggregation/precipitation, dispersibility, solubility, compatibility, wettability, stability, adsorption, etc. ☆ Fatigue testing / tensile strength, temperature dependence, viscoelasticity, modulus of elasticity, viscosity, stress relaxation, creep, master curve, residual strain, etc.

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イプロス_サムネイル(タンパク質分析_NanoDSC).jpg

Analysis case: Evaluation of protein denaturation temperature and Zn2+ effects using NanoDSC.

This is an introduction to a protein analysis case study by a specialist in analysis. This time, we evaluated the changes in denaturation temperature behavior with the addition of Zn2+ using NanoDSC.

Measurement capabilities offered by TA Instruments ☆ Thermal analysis / glass transition, crystallization, thermogravimetric analysis, curing reactions, expansion rate, thermal conductivity, shrinkage rate, effects of moisture, etc. ☆ Viscoelastic measurements (rheology) / viscoelasticity, elastic modulus, viscosity, stress relaxation, creep, master curve, residual strain, molecular weight, molecular weight distribution, etc. ☆ Microcalorimetry / intermolecular interactions, crystallization, amorphousness, aggregation/precipitation, dispersibility, solubility, compatibility, wettability, stability, adsorption, etc. ☆ Fatigue testing / tensile strength, temperature dependence, viscoelasticity, elastic modulus, viscosity, stress relaxation, creep, master curve, residual strain, etc.

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image_25.jpg

Isothermal Titration Calorimeter (Affinity ITC)

Provides high reliability! An excellent tool for measuring a wide range of molecular interactions.

The Isothermal Titration Calorimeter (ITC) is a device that detects the heat changes associated with titration at a constant temperature and is primarily used for analyzing molecular interactions. It measures the minute heat changes that occur when molecules bind to each other, allowing for the determination of binding ratio (n), binding constant (Ka), and the enthalpy change of binding (ΔH) from the resulting titration curve. Affinity ITC is an isothermal titration calorimeter that provides high sensitivity, high reproducibility, and advanced ITC technology in life science research environments. With AccuShot technology, it delivers precise amounts of titrant to the appropriate location, and the FlexSpin stirring function achieves efficient mixing and high sensitivity. The automatic cleaning function prevents contamination between samples. It offers the option of standard capacity (1.0 mL) or low capacity cells (190 µL), and a temperature-controlled auto-sampler for 96 wells is also available.

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イプロス_サムネイル(Youtube資料)DSC1.jpg

Youtube Lecture Materials ~DSC Basic Edition 1~

This is the lecture material posted on YouTube ~DSC Basic Edition 1~. It is available for free download, so please feel free to make use of it.

Data Content This is a PDF version of the lecture materials from YouTube, converted for easier viewing. The video consists of a total of 12 pages and provides an easy-to-understand explanation for beginners about: - Principles - Analytical methods - Enthalpy relaxation (recovery) I think it might be difficult to understand with just the lecture materials, so please also refer to the YouTube videos below. YouTube TA Summary Site: https://www.youtube.com/channel/UCk3XzqRdsNQz1hJlqKrfujQ DSC Basics Part 1: https://www.youtube.com/watch?v=DbGpordpweg

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[Notice of Exhibition Participation by Fukuda Co., Ltd.] JAPAN PACK 2025 Japan Packaging Industry Exhibition

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We would like to extend our heartfelt congratulations on the continued prosperity of your esteemed company. Our company will be exhibiting at the following trade show, where we will introduce a number of products, including our new product, the Pillow Packaging Full Air Tightness Inspection Machine MSQ-2003. We sincerely apologize for the inconvenience during your busy schedule, but we would like to invite you to visit us at this opportunity. 【 Exhibition Name 】 JAPAN PACK 2025 Japan Packaging Industry Exhibition https://www.japanpack.jp/ 【 Dates 】 October 7 (Tuesday) to October 10 (Friday), 2025 *4 days 10:00 AM to 5:00 PM 【 Venue / Booth Number 】 Tokyo Big Sight / East Hall 8, Booth 8-505

Sep 03, 2025

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■Notice of the "2024 Attack Fair in Ichihara"!■ 【December 11, 2024 (Wednesday) to December 12, 2024 (Thursday)】

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Nitto Kohki will hold the "2024 Attack Fair in Ichihara" at the "Yume Hall" for two days from December 11 (Wednesday) to December 12 (Thursday), 2024. The Attack Fair is an exhibition where you can actually "see, touch, and operate" Nitto Kohki's products. Additionally, there will be wonderful souvenirs for visitors and many fun activities prepared. We look forward to welcoming everyone!

Sep 01, 2025

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■Notice of the "2025 Attack Fair in Kurume"!■ 【February 13, 2025 (Thursday) to February 14, 2025 (Friday)】

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Nitto Kohki will hold the "2025 Attack Fair in Kurume" at the "Local Production Kurume General Exhibition Hall" for two days from February 13 (Thursday) to February 14 (Friday), 2025. The Attack Fair is an exhibition where you can actually "see, touch, and operate" Nitto Kohki's products. Additionally, there will be wonderful commemorative gifts for visitors and many fun activities prepared. We look forward to welcoming everyone!

Sep 01, 2025

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If the methods of supervisors and veteran employees don't resonate... A must-see for companies that want to solve the high turnover rate of young employees!!

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**Seminar Overview** The construction industry faces many challenges, including a shortage of personnel, regulations on maximum working hours, and the need for efficiency improvements. Additionally, the transmission of skills due to an aging workforce has become a significant issue, with a high turnover rate among younger employees who are supposed to carry on these skills. In this seminar, we will discuss the causes of turnover based on case studies and explain key points on how to reduce turnover rates by advancing IT integration.

Sep 01, 2025

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The differences in generative AI depend on the user... What are the secrets to using it effectively?

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**Seminar Overview** "Generative AI," which is gaining attention as a method for improving business efficiency and utilizing internal digital transformation (DX), can be influenced by the literacy of the employees using it, potentially increasing the burden of work instead. In this seminar, we will discuss the current challenges of generative AI while providing examples of how it can be used to enhance business efficiency. We will also explain how to utilize generative AI in a way that achieves efficiency without being dependent on the user's skills. We hope this will serve as a reference for considering the use of generative AI.

Sep 01, 2025

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