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  1. Home
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  3. TA Instruments Japan (TAInstruments)
  4. July Online LIVE Seminar! ~Protein Analysis Seminar~
SEMINAR_EVENT
  • Jun 08, 2022
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Jun 08, 2022

July Online LIVE Seminar! ~Protein Analysis Seminar~

TA Instruments Japan TA Instruments Japan (TAInstruments)
13:00~14:00 Analysis of the interaction between sleep substance-synthesizing enzyme and ligand using isothermal titration calorimetry Shigeru Shimamoto, Lecturer, Department of Life Science, College of Science and Engineering, Kinki University Isothermal titration calorimetry (ITC) has the significant advantage of detecting molecular interactions in a form that is closer to the state present in vivo, without the need for chemical modifications, immobilization, or detection probes. Furthermore, it is a method that provides nearly complete thermodynamic parameters related to the interactions. Here, I will introduce the analysis of the interaction between lipocalin-type prostaglandin D synthase (L-PGDS), which acts as a sleep substance-synthesizing enzyme in the mammalian brain, and its ligand, prostaglandin (PG), using ITC, and discuss the elucidation of the enzyme reaction mechanism in conjunction with structural information. 14:00~14:30 Introduction to isothermal titration calorimetry (ITC) and differential scanning calorimetry (DSC) for biomacromolecules Minako Maeda, Applications Department, TA Instruments
Date and time Friday, Jul 22, 2022
01:00 PM ~ 02:30 PM
Please apply through the link.
Capital
Entry fee Free Thank you very much for your participation.
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Discovery DSC.jpg

Differential Scanning Calorimeter Discovery DSC (Calorimeter)

How about one for the lab? Introducing the Discovery DSC series, the world's most outstanding and highly versatile differential scanning calorimeter.

TA Instruments continues to be the leading company in the world of DSC with innovative and outstanding technology. It provides reliable data and enhances workflow and productivity in laboratories. Material property analysis is essential at all stages of product design, manufacturing, processing, and application. Differential Scanning Calorimetry (DSC) is the most widely used thermal analysis instrument for evaluating the physical properties of a wide range of materials, including polymers, pharmaceuticals, food, biomaterials, organic compounds, inorganic compounds, and composites.

  • Analytical Equipment and Devices

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Collection of Thermal Analysis Case Studies for Protein Analysis (Calorimeter) ITC DSC

This is an introduction to protein analysis cases and related equipment by analysis specialists. This time, we will cover three devices: ITC, TAM, and NanoDSC!

Measurement capabilities offered by TA Instruments ☆ Thermal analysis / glass transition, crystallization, thermogravimetric analysis, curing reactions, expansion rate, thermal conductivity, shrinkage rate, effects of moisture, etc. ☆ Viscoelastic measurements (rheology, rheometers) / viscoelasticity, elastic modulus, viscosity, stress relaxation, creep, master curves, residual strain, molecular weight, molecular weight distribution, etc. ☆ Microcalorimetry / intermolecular interactions, crystallization, amorphousness, aggregation/precipitation, dispersibility, solubility, compatibility, wettability, stability, adsorption, etc. ☆ Fatigue testing / tensile strength, temperature dependence, viscoelasticity, elastic modulus, viscosity, stress relaxation, creep, master curves, residual strain, etc.

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1-DSC250_MicroCamera-Detail_jpg.jpg

Accessory for Differential Scanning Calorimeter (DSC) - Microscope Camera

Device accessories

The DSC measurements conducted with microscope accessories provide insights through multiple applications to better understand the properties and behaviors of materials. It is possible to capture images related to crystallization and melting transitions, flow of materials above the glass transition and melting points, phase transitions, and volume dimensional changes associated with evaporation and sublimation. Visual observation of color changes and other appearance alterations is used in combination with DSC data to gain a deeper understanding of the material being analyzed.

  • Color camera

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イプロス_サムネイル(タンパク質分析_ITC).jpg

Analysis case: Evaluation of substrate decomposition rate and inhibitor efficacy by ITC.

This is an introduction to a protein analysis case study by a specialist in analysis. This time, we evaluated the substrate degradation rate and the effects of inhibitors using ITC.

Measurement capabilities offered by TA Instruments ☆ Thermal analysis / glass transition, crystallization, thermogravimetric analysis, curing reactions, expansion rate, thermal conductivity, shrinkage rate, effects of moisture, etc. ☆ Viscoelastic measurement (rheology) / viscoelasticity, modulus of elasticity, viscosity, stress relaxation, creep, master curve, residual strain, molecular weight, molecular weight distribution, etc. ☆ Microcalorimetry / intermolecular interactions, crystallization, amorphousness, aggregation/precipitation, dispersibility, solubility, compatibility, wettability, stability, adsorption, etc. ☆ Fatigue testing / tensile strength, temperature dependence, viscoelasticity, modulus of elasticity, viscosity, stress relaxation, creep, master curve, residual strain, etc.

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イプロス_サムネイル(タンパク質分析_NanoDSC).jpg

Analysis case: Evaluation of protein denaturation temperature and Zn2+ effects using NanoDSC.

This is an introduction to a protein analysis case study by a specialist in analysis. This time, we evaluated the changes in denaturation temperature behavior with the addition of Zn2+ using NanoDSC.

Measurement capabilities offered by TA Instruments ☆ Thermal analysis / glass transition, crystallization, thermogravimetric analysis, curing reactions, expansion rate, thermal conductivity, shrinkage rate, effects of moisture, etc. ☆ Viscoelastic measurements (rheology) / viscoelasticity, elastic modulus, viscosity, stress relaxation, creep, master curve, residual strain, molecular weight, molecular weight distribution, etc. ☆ Microcalorimetry / intermolecular interactions, crystallization, amorphousness, aggregation/precipitation, dispersibility, solubility, compatibility, wettability, stability, adsorption, etc. ☆ Fatigue testing / tensile strength, temperature dependence, viscoelasticity, elastic modulus, viscosity, stress relaxation, creep, master curve, residual strain, etc.

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image_25.jpg

Isothermal Titration Calorimeter (Affinity ITC)

Provides high reliability! An excellent tool for measuring a wide range of molecular interactions.

The Isothermal Titration Calorimeter (ITC) is a device that detects the heat changes associated with titration at a constant temperature and is primarily used for analyzing molecular interactions. It measures the minute heat changes that occur when molecules bind to each other, allowing for the determination of binding ratio (n), binding constant (Ka), and the enthalpy change of binding (ΔH) from the resulting titration curve. Affinity ITC is an isothermal titration calorimeter that provides high sensitivity, high reproducibility, and advanced ITC technology in life science research environments. With AccuShot technology, it delivers precise amounts of titrant to the appropriate location, and the FlexSpin stirring function achieves efficient mixing and high sensitivity. The automatic cleaning function prevents contamination between samples. It offers the option of standard capacity (1.0 mL) or low capacity cells (190 µL), and a temperature-controlled auto-sampler for 96 wells is also available.

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Youtube Lecture Materials ~DSC Basic Edition 1~

This is the lecture material posted on YouTube ~DSC Basic Edition 1~. It is available for free download, so please feel free to make use of it.

Data Content This is a PDF version of the lecture materials from YouTube, converted for easier viewing. The video consists of a total of 12 pages and provides an easy-to-understand explanation for beginners about: - Principles - Analytical methods - Enthalpy relaxation (recovery) I think it might be difficult to understand with just the lecture materials, so please also refer to the YouTube videos below. YouTube TA Summary Site: https://www.youtube.com/channel/UCk3XzqRdsNQz1hJlqKrfujQ DSC Basics Part 1: https://www.youtube.com/watch?v=DbGpordpweg

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PIAB [Booth: West Hall 2 W2-32] will be exhibiting at iREX 2025 "2025 International Robot Exhibition"!

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[Exhibition Display] Demonstration of pineapple adhesion dynamics using collaborative robots Dynamic display using collaborative robots and tool changers for gripping Static display of a 5kg dual-sided gripper Comparison of ejector compressed air consumption Various suction pads Foam gripper, etc. We recommend visiting on the first or second day when you can view the exhibits with relative ease. *The third day (Friday) is expected to be very crowded. 【Gripper Sale Event】 We will be selling the following two products at a discounted price at the exhibition booth. Please inquire about the special prices at the venue. ■ 'KENOS Gripper KVGL.400.100, N214.FR6.S2' URL: https://www.youtube.com/watch?v=HsNRwrDtjW0 ■ 'Collaborative Robot Hand KCS.Q110.N301.110.FR6.SX422.V1.X.X' URL: https://www.youtube.com/watch?v=GFKg3blZzdQ These products are ideal not only for actual applications but also for use as test machines. How about taking this opportunity?

Dec 05, 2025

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Notice of Participation in SEMICON JAPAN 2025

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Watanabe Trading Company will be exhibiting at "SEMICON Japan 2025" in collaboration with its group companies, Amaya Manufacturing, Wacom Manufacturing, and Tomco. At our booth, we will introduce quartz chambers, silicone rubber, surface modification processing, and the AI visual inspection device "NulMiL," among others. We look forward to your visit.

Dec 03, 2025

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[Seminar] Latest Trends and Initiatives/Outlook of Two Companies Towards the Social Implementation of Methanation

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[Lecture Topics] 1. Gas Vision 2050 and Action Plan 2030, and efforts to build a carbon-neutral gas value chain Ryota Kōgetsu, Senior Researcher (International Standards) at the Japan Gas Association 2. Osaka Gas's efforts in developing innovative methanation technology Yōsuke Kuwahara, Director of the Carbon Neutral Promotion Office, Planning Department, Management Planning Division at Osaka Gas Co., Ltd. 3. Methanation efforts by Tokyo Gas aiming for carbon neutrality from the perspective of hydrogen trends Hisataka Yakabe, Executive Fellow at the Green Transformation Company, Tokyo Gas Co., Ltd.

Dec 03, 2025

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[Seminar] Management Strategies Utilizing Intellectual Property to Generate Sustainable Profitability

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[Instructor] Kikaku Corporation, Nomura Research Institute, Consulting Division Senior Principal, Patent Attorney Rikiichi Hayashi [Key Lecture Content] In today's manufacturing industries, including electronics, machinery, chemicals, semiconductors, batteries, pharmaceuticals, telecommunications, automobiles, food, steel, cement, convenience stores, and content IP, the intensification of global competition and the rise of emerging companies leading to commoditization are accelerating. It is becoming increasingly difficult to secure sustainable profits with traditional exclusive intellectual property strategies alone. In this seminar, we will systematize the management model practiced by winners as the "IPX Management Strategy Model." By redefining and clarifying the functions of Open IP (customer-attracting IP) in addition to traditional Closed IP (revenue-generating IP), we will explain specific methods for management transformation that create asymmetric advantages (business disruption power) that overwhelm competitors. We will share practical approaches that achieve an operating profit margin of over 15% and a PBR of 2-3 times.

Dec 03, 2025

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December 18th | University-Originated Seed Matching Seminar: A Special Feature on Next-Generation Life Sciences Supported by "Visualization" Technology

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We will hold the 29th University-Originated Seed Matching Seminar. We will introduce the latest technologies from Kyoto University. We hope you will join us. 【▼Details here (Free participation, online)】 https://www.tlo-kyoto.co.jp/event/202511191448.html ━━━━━━━━━━━━━━━━━━━━ ◯ Date and Time: December 18, 2025 (Thursday) 13:30–15:00 ◯ Application Deadline: December 12, 2025 (Friday) ◯ Format: Online, Free ━━━━━━━━━━━━━━━━━━━━ ▼ Program Around 13:30–14:00: “Automatic Detection System for Muscle Activation Intervals for Home Monitoring of Sleep Disruption Factors” Lecturer Kana Eguchi, Graduate School of Informatics, Kyoto University Around 14:00–14:30: “Quantitative Evaluation of Antibody Drug Stability Against Fluid Stress” Professor Kenji Sugase, Graduate School of Agriculture, Kyoto University Around 14:30–15:00: “High-Throughput, Low-Cost Comprehensive Transcriptome Analysis Enabled by Nearly Random Index (NRI) Method” Professor Yasuhiro Murakawa, Graduate School of Medicine / ASHBi, Kyoto University

Dec 03, 2025

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