This is an introduction to the standard protocol for inducing atopic dermatitis using BioSta AD.
In the atopic dermatitis model using Biosta AD, after destroying the skin barrier with 4% SDS, the product is applied to the back and ear area of the mice at a dose of 100 mg/mouse twice a week. The induction period is approximately 3 weeks (for a total of 6 applications). Ultimately, a total of 600 mg of the product will be applied. In this model, symptoms begin to appear from 1 to 2 weeks after the start of application, with an incidence rate of about 80% observed at 3 to 4 weeks. 【Initial Induction Method】 ○(1) NC/Nga mice (female, from Charles River Japan, aged 10 weeks or older) are anesthetized (with ether), and the back and ear areas are shaved with clippers, followed by the application of an appropriate amount of depilatory agent to remove hair. ○(2) After wiping off the depilatory agent, 100 mg of Biosta AD is evenly applied to the back and ear areas. ○(3) After application, the mice are returned to their breeding cages. For more details, please contact us or download the catalog.
Inquire About This Product
basic information
【Induction Method After the Second Time】 ○(1) If hair growth has been confirmed, it is essential to shave using clippers and/or an electric shaver. ○(2) Apply 150 μl of 4% SDS solution evenly by dropping it onto the back and ear area using a micropipette. ○(3) Dry to some extent using a hairdryer (cool air). ○(4) After returning the mouse to the breeding cage, leave it for about 2 to 3 hours to dry naturally. ○(5) Apply 100 mg of BiostaAD evenly to the back and ear area. ○(6) After application, return the mouse to the breeding cage. *All treatments are performed twice a week. Atopic dermatitis is induced over 3 weeks with a total of 6 treatments. (The final 6th induction occurs on the 17th or 18th day after the initial induction. Scoring is done on the 21st day, and the subjects are used for experiments.) ● For more details, please contact us or download the catalog.
Price information
Please contact us.
Delivery Time
※Please contact us.
Applications/Examples of results
For more details, please contact us or download the catalog.
catalog（1）
Download All Catalogs
Company information
In modern society, allergies are a very common illness. Many of you may have family or friends who are struggling with this condition. The causes of allergies are diverse, including dust mites, house dust, pollen, and food, among others. All of these are inseparable from everyday life, and it is impossible to live normally while isolating oneself from these allergens. While medication is the mainstream treatment for allergies, it is also important to prevent symptoms by improving the living environment to reduce the entry of allergens into the body. It is crucial to suppress allergies comfortably and safely. To achieve this, it is necessary to utilize a wide range of technologies. BioSta Inc. (bio: biotechnology, stir: to stir/mix) was named with the intention of tackling this challenge by mixing various technologies and products.