Successful measurement of the concentration distribution of low molecular weight compounds administered to mouse tissues: A new method for elucidating drug action mechanisms in living organisms.
● We have developed a method to measure the concentration distribution of low molecular weight compounds in moist tissues. ● A pathway has been opened for the localization measurement of drugs and low molecular weight bioactive substances in biological tissues. ● In the future, applications for elucidating the mechanisms of action of low molecular weight bioactive substances and developing new therapeutic drugs are expected. 【Features of the device】 1. Device adjustment frequency -------------------- Continuous, no adjustment needed 2. Measurement time after power on ----------- Immediate measurement possible 3. Time required for initial measurement adjustment after installation ------- Less than 2 hours 4. Direct measurement ---------------- Measurement without using fluorescent substances 5. Compact size, cost reduction ------------ Composed of only one femtosecond laser 【Samples and specimens that can utilize this technology】 1. Non-destructive, non-contact measurement 2. Identification of molecular structures 3. Measurement of depth distribution of samples 4. Samples containing moisture at room temperature in the atmosphere 5. Plants, insects, fish, etc. (Note: Some measurement objects may not be measurable)
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basic information
The inherent molecular vibration spectrum (Raman spectrum) of low molecular weight compounds was measured, and the shape of that spectrum was used as a marker. As a method for measuring this molecular vibration spectrum, we developed a "phase-controlled coherent Raman microscopy" that uses pulse laser light with manipulated spectral phases, achieving high sensitivity. It has become possible to detect molecular vibration spectra in small frequency regions, which was previously difficult, with high sensitivity.
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P8
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Applications/Examples of results
Analysis of small molecules (low molecular weight compounds) such as steroids and taurine.
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