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  6. Wide-pore HILIC column for glycoprotein analysis

Wide-pore HILIC column for glycoprotein analysis

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last updated:Jan 13, 2016

日本ウォーターズ(米国本社 Waters Corporation) 
日本ウォーターズ(米国本社 Waters Corporation) 
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Isolate glycoproteins based on the number and composition of sugar chains! Glycoprotein column

Glycosylation of proteins is a very important post-translational modification that regulates both the structure and function of proteins. Changes in glycan profiles can affect drug efficacy and immunogenicity, so glycosylated biopharmaceuticals require thorough characterization. In the characterization of glycoproteins, the wide-pore HILIC amide column (Glycoprotein BEH Amide, 300Å), which provides complementary information with reverse phase columns, allows for the separation of intact glycoproteins, glycoprotein subunits, or at the glycopeptide level based on the number and composition of glycans, enabling the acquisition of unprecedented glycoform information.

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Glycoproteinカラム1.jpg

Wide-pore HILIC column for glycoprotein analysis

Glycoproteinカラム1.jpg
Glycoproteinカラム1.jpg Glycoproteinカラム2.jpg
  • Waters Glycan Column Homepage
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■Optimized wide-pore HILIC stationary phase for the separation of glycoforms from intact and digestible glycopeptides ■Generation of information on domain-specific glycosylation, regardless of MS detection ■Analysis of site-specific glycan occupancy ■High-resolution peptide mapping independent of peptide/glycan size ■Improved separation of large free N-linked glycans ■Conducting QC tests on filler gel batches using Glycoprotein Performance Test Standard

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  • Glycoproteinカラム2.jpg

    Glycoprotein column Analysis of glycoprotein characteristics possible with the glycoprotein column

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Glycoprotein Column [ACQUITY UPLC Glycoprotein BEH Amide 300Å Column]

Glycoprotein Column [ACQUITY UPLC Glycoprotein BEH Amide 300Å Column]

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[On-Demand Webinar] LC Course Starting Here - Intermediate Level: Mastering HILIC Mode

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Compounds or their metabolites found in pharmaceuticals, cosmetics, and food can have high hydrophilicity. In environmental monitoring, hydrophilic and highly polar compounds may also be targeted in water system environmental analysis. Since these compounds are difficult to analyze using conventional reverse phase chromatography, hydrophilic interaction chromatography (HILIC) is often suitable. On the other hand, many people may think, "It sounds complicated just by the name," and have not yet tried HILIC, or they may not be satisfied with their current HILIC analysis. In this seminar, we will introduce the principles of HILIC analysis, key points for method development, and columns suitable for HILIC analysis. Program: ■ Basics of HILIC mode ■ Development of HILIC analytical methods ■ Columns suitable for HILIC mode and analysis examples Target Audience: ■ Those analyzing highly polar and hydrophilic compounds ■ Those planning to conduct analyses in the future ■ Those considering improvements to their current HILIC analysis

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日本ウォーターズ(米国本社

日本ウォーターズ(米国本社 Waters Corporation) 

Testing, Analysis and Measurement

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Waters Corporation, headquartered in Milford, Massachusetts, USA, has played a pioneering role as a specialized manufacturer of liquid chromatography since its establishment in 1958. Actively engaged in the development of new products and technologies, it has grown into an international group company that supplies not only high-performance liquid chromatography but also mass spectrometers (MS) and thermal analysis instruments. Waters Japan started operations in 1973 (Showa 48) as the Japanese base of Waters Corporation. Inheriting the corporate ethos of the U.S. headquarters, we have made "Customer Success" our mission and continue to provide innovative technologies and lab solutions.

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