Sample preparation for the analysis of thyroid hormones in serum using LS-MS/MS with EVOLUTE EXPRESS AX.
This application note describes a sample preparation method using polymer-based strong anion exchange mixed-mode solid-phase extraction for the analysis of thyroid hormones T3, rT3, and T4 in serum by LC-MS/MS. Using the EVOLUTE EXPRESS AX 30 mg fixed well plate format, thyroid hormones (T3, rT3, and T4) were extracted from 200 μL of serum. A high recovery rate with good reproducibility was achieved, while minimizing the co-elution of phospholipids, resulting in a quantification limit of less than 50 pg/mL. *You can download the English version of the catalog. *For more details, please refer to the PDF document or feel free to contact us.
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*You can download the English version of the catalog. *For more details, please refer to the PDF document or feel free to contact us.
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*You can download the English version of the catalog. *For more details, please refer to the PDF document or feel free to contact us.
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Biotage Japan Co., Ltd. is a wholly-owned subsidiary of Biotage AB, which is headquartered in Uppsala, Sweden (BIOT: NASDAQ/OMX Stockholm). Biotage AB has its roots in several venture companies that originated from Uppsala University, a leading science and engineering university in the Nordic region. After undergoing international acquisitions and restructuring throughout the 2000s, it has recently achieved remarkable growth as a global company.
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