You can directly quantify exosomes from blood samples and cell culture supernatants!
These are three types of products for the relative quantification of exosomes containing CD9, CD63, or CD81 molecules. - CD9/CD9 Exosome ELISA Kit - CD63/CD63 Exosome ELISA Kit - CD81/CD81 Exosome ELISA Kit Features: - Exosomes contained in blood samples or cell culture supernatants (serum-free) can be directly quantified. - No special equipment is required; measurements can be made with a standard plate reader. - Instead of using exosomes themselves, which lack storage stability as standard reagents, stability and reproducibility are ensured by using standard beads with a particle size of 200 nm that are coated with CD9 or CD63. - Relative quantification of each sample is possible by correcting with CD9, CD63, or CD81 standard beads that mimic exosome structures. - Exosomes are captured using solid-phase CD9, CD63, or CD81 antibodies, and detected with HRP-labeled CD9, CD63, or CD81 antibodies.
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basic information
Contents: Solid-phase 96-well plates coated with anti-CD9 antibody, anti-CD63 antibody, or anti-CD81 antibody, 8-well x 12 strips, 1 piece CD9, CD63, or CD81 standard beads, 200μL, 1 vial Assay buffer, 25mL, 1 vial Washing buffer (10x), 25mL, 1 vial HRP-labeled anti-CD9, anti-CD63 antibody, or anti-CD81 antibody (500x), 20μL, 1 vial Substrate solution, 12mL, 1 vial Stop solution (2N H2SO4), 6mL, 1 vial Plate seals, 2 pieces *For more details, please download the catalog or feel free to contact us.
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Applications/Examples of results
Source: Human Application: Enzyme Linked Immunosorbent Assay Measurement Range: CD9 positive exosomes; 0.281–18 ng/mL CD63 positive exosomes; 0.625–40 ng/mL CD81 positive exosomes; 7.813–250 ng/mL Purpose: This product can relatively quantify exosomes that have CD9, CD63, or CD81 molecules on their surface using a sandwich ELISA with high-performance antibodies against the exosome markers CD9, CD63, or CD81.
Line up（3）
| Model number | overview |
|---|---|
| HAK-HEL0909-1 | Human-derived exosome quantification CD9/CD9 ELISA kit |
| HAK-HEL6363-1 | Human-derived exosome quantification CD63/CD63 ELISA kit |
| HAK-HEL8181-1 | Human-derived exosome quantification CD81/CD81 ELISA kit |
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Company information
Hakarel Co., Ltd. primarily supports research on exosome detection methods and offers contract services such as the production of recombinant proteins, the creation of monoclonal antibodies, the establishment of ELISA measurement systems, exosome ELISA measurements, and proteomics analysis. We respond flexibly to customer requests and provide support for research and development. Please feel free to contact us if you have any inquiries.