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  6. Human-derived exosome quantification ELISA kit

Human-derived exosome quantification ELISA kit

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last updated:Nov 07, 2022

ハカレル
ハカレル
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You can directly quantify exosomes from blood samples and cell culture supernatants!

These are three types of products for the relative quantification of exosomes containing CD9, CD63, or CD81 molecules. - CD9/CD9 Exosome ELISA Kit - CD63/CD63 Exosome ELISA Kit - CD81/CD81 Exosome ELISA Kit Features: - Exosomes contained in blood samples or cell culture supernatants (serum-free) can be directly quantified. - No special equipment is required; measurements can be made with a standard plate reader. - Instead of using exosomes themselves, which lack storage stability as standard reagents, stability and reproducibility are ensured by using standard beads with a particle size of 200 nm that are coated with CD9 or CD63. - Relative quantification of each sample is possible by correcting with CD9, CD63, or CD81 standard beads that mimic exosome structures. - Exosomes are captured using solid-phase CD9, CD63, or CD81 antibodies, and detected with HRP-labeled CD9, CD63, or CD81 antibodies.

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Human-derived exosome quantification ELISA kit

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Contents: Solid-phase 96-well plates coated with anti-CD9 antibody, anti-CD63 antibody, or anti-CD81 antibody, 8-well x 12 strips, 1 piece CD9, CD63, or CD81 standard beads, 200μL, 1 vial Assay buffer, 25mL, 1 vial Washing buffer (10x), 25mL, 1 vial HRP-labeled anti-CD9, anti-CD63 antibody, or anti-CD81 antibody (500x), 20μL, 1 vial Substrate solution, 12mL, 1 vial Stop solution (2N H2SO4), 6mL, 1 vial Plate seals, 2 pieces *For more details, please download the catalog or feel free to contact us.

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P2

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Applications/Examples of results

Source: Human Application: Enzyme Linked Immunosorbent Assay Measurement Range:   CD9 positive exosomes; 0.281–18 ng/mL   CD63 positive exosomes; 0.625–40 ng/mL   CD81 positive exosomes; 7.813–250 ng/mL Purpose: This product can relatively quantify exosomes that have CD9, CD63, or CD81 molecules on their surface using a sandwich ELISA with high-performance antibodies against the exosome markers CD9, CD63, or CD81.

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Model number overview
HAK-HEL0909-1 Human-derived exosome quantification CD9/CD9 ELISA kit
HAK-HEL6363-1 Human-derived exosome quantification CD63/CD63 ELISA kit
HAK-HEL8181-1 Human-derived exosome quantification CD81/CD81 ELISA kit

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Human-derived exosome quantification ELISA kit (new product flyer)

Human-derived exosome quantification ELISA kit (new product flyer)

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Human PD-L1 Positive Exosome (CD9) ELISA Kit Flyer

Human PD-L1 Positive Exosome (CD9) ELISA Kit Flyer

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Human EpCAM Positive Exosome (CD9) ELISA Kit Flyer

Human EpCAM Positive Exosome (CD9) ELISA Kit Flyer

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BioJapan 2019

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We will be exhibiting at BioJapan 2019. It is the largest partnering event in Asia for the bio business, held every autumn in Yokohama. Exhibition Booth: Osaka Bio Headquarters (Secretariat: Osaka Prefecture) Booth Number: B-12 We will be showcasing and displaying brochures of Hakarel's services and products. Please be sure to stop by our booth at Booth Number: B-12!

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Hakarel Seminar

Exosome Technical Seminar

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Exosome secretion from cells is gaining significant attention worldwide as a carrier for intercellular communication in living organisms, disease diagnosis, and drug delivery. This seminar will feature a technical presentation on liquid biopsy using exosomes by the representative director of Hakarel Co., Ltd. Participation is free for the first 50 attendees, and there will be participant benefits. We encourage you to take this opportunity to join us. Date and Time: October 2, 2019 (Wednesday), 16:00 - 17:00 Location: Meeting Room, 2nd Floor, Saito Biohills Center (7-7-18 Asagi, Ibaraki City) Lecture Content: Title: "Immunoassays for Exosome Surface Antigens and Their Applications" - Quantification of exosomes using ELISA with anti-tetraspanin antibodies - Liquid biopsy through quantification of PD-L1 positive exosomes - Screening method for anti-membrane protein antibodies using exosome ELISA, etc. Speaker: Mitsu Sonoda Representative Director, Hakarel Co., Ltd. / Director, Japanese Society for Extracellular Vesicles Participation: - Pre-registration required - Limited to the first 50 attendees - Free of charge - Participant benefits available

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ハカレル

ハカレル

Pharmaceuticals and Biotechnology

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Hakarel Co., Ltd. primarily supports research on exosome detection methods and offers contract services such as the production of recombinant proteins, the creation of monoclonal antibodies, the establishment of ELISA measurement systems, exosome ELISA measurements, and proteomics analysis. We respond flexibly to customer requests and provide support for research and development. Please feel free to contact us if you have any inquiries.

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