PD-L1 positive exosomes can be directly quantified from blood samples and cell culture supernatants using the ELISA method!
This product is a sandwich ELISA kit that utilizes high-performance antibodies against CD9, an exosome marker, and PD-L1, an immune checkpoint-related molecule, to detect PD-L1 molecules expressed on the surface of exosomes secreted by cells in human blood and cell culture supernatants. PD-L1 is also expressed on exosomes, adopting the same topology as PD-L1 on the surface of cancer cells, with the extracellular domain exposed on the surface of the exosomes. Features: - Highly sensitive detection of human PD-L1 positive exosomes using the ELISA method (detection sensitivity: 0.05 ng/mL) - Direct quantification of PD-L1 positive exosomes contained in human blood samples and cell culture supernatants - No special equipment is required; measurements can be taken with a standard plate reader - Instead of using exosomes themselves, which lack storage stability as standard reagents, stability and reproducibility are ensured by using PD-L1/CD9 fusion protein (standard protein) - Relative quantification of each sample is possible by reading a standard curve using PD-L1/CD9 fusion protein (standard protein)
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basic information
Contents: Anti-CD9 antibody solid-phase 96-well plate, 8-well x 12 strips, 1 piece Standard protein (PD-L1/CD9 fusion protein), 100μL, 1 vial Assay buffer, 25mL, 1 vial Washing buffer (10x), 25mL, 1 vial HRP-labeled anti-CD9 antibody (500x), 20μL, 1 vial Substrate solution, 12mL, 1 vial Stop solution (2N H2SO4), 6mL, 1 vial Plate seal, 1 piece *For more details, please download the latest data sheet from Hakarel's website or feel free to contact us. (As of January 2020, the contents and operating procedures have been changed.)
Price range
P2
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Applications/Examples of results
Origin: Human Application: Enzyme Linked Immunosorbent Assay Applicable Samples: Serum, Plasma, Cell culture supernatant Measurement Range (Standard Curve Range): 0.156 to 10 ng/mL Sensitivity (Standard Substance): 0.05 ng/mL Purpose: This kit allows for the high-sensitivity direct quantification of human PD-L1 positive exosomes in samples using a sandwich method with high-performance antibodies against CD9, an exosome marker, and PD-L1, an immune checkpoint-related molecule.
Detailed information
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This ELISA kit is based on the sandwich method. The ELISA plate in the kit is pre-coated with anti-CD9 antibodies, and when a sample is added, the exosomes in the sample are trapped. After washing, an HRP-conjugated anti-PD-L1 antibody is reacted with the PD-L1 expressed on the trapped exosomes, and after adding the substrate, the color development by HRP is read and quantified using a plate reader.
Line up（1）
| Model number | overview |
|---|---|
| HAK-HELPDL109-1 | Human PD-L1 positive exosome (CD9) ELISA kit |
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Company information
Hakarel Co., Ltd. primarily supports research on exosome detection methods and offers contract services such as the production of recombinant proteins, the creation of monoclonal antibodies, the establishment of ELISA measurement systems, exosome ELISA measurements, and proteomics analysis. We respond flexibly to customer requests and provide support for research and development. Please feel free to contact us if you have any inquiries.
