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  6. Single Cell Western Milo

Single Cell Western Milo

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Milo

last updated:Mar 17, 2025

プロテインシンプルジャパン
プロテインシンプルジャパン
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Latest protein expression analysis device

Milo is a cutting-edge device that can analyze protein expression at the single-cell level. It can identify subpopulations of cells that cannot be observed with traditional Western blotting. In other words, it allows for the study of specific cell types within heterogeneous samples. Milo performs single-cell Westerns (scWesterns) on over 1,000 cells in a single measurement. In a significantly shorter time than traditional Western blotting, it provides expression information for up to four types of proteins from a single cell, enabling the analysis of characteristic behaviors, expressions, and variations within small populations of cells in the sample.

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Single Cell Western Milo

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  • Related Link - https://www.bio-techne.com/p/single-cell-western/m…

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basic information

**Features** - Analysis of heterogeneity in multicellular populations and cell populations sorted by FACS - Analysis of heterogeneity based on isoforms and expression levels - Identification of cell subpopulations - Identification of differentiated cell subtypes - Simplification of phospho-flow analysis - Detection of proteins at the single-cell level using antibodies that are difficult to use in flow cytometry - Analysis of single-cell RNA at the protein level - Single-cell level analysis of signaling pathways obtained through gene enrichment analysis and others

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Applications/Examples of results

It is being introduced to cutting-edge research sites such as Johns Hopkins University, Cold Spring Harbor Laboratory, the FDA, NIH, Stanford University, and the University of Tokyo.

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Analytical solutions for the development and manufacturing of cell and gene therapies and biopharmaceuticals.

Analytical solutions for the development and manufacturing of cell and gene therapies and biopharmaceuticals.

TECHNICAL
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Comprehensive Catalog 2025 Edition

Comprehensive Catalog 2025 Edition

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COMET application

About the release of the Comprehensive Catalog 2025 edition.

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A high-throughput fully automated staining and imaging device capable of simultaneous detection of RNA and proteins. The single-cell spatial multi-omics COMET is also newly listed.

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Bio-Techne showcases innovative cancer research, cell therapy, and manufacturing tools at the AACR 2023 conference.

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Minneapolis / PRNewswire / April 13, 2023 (local U.S. time) Bio-Techne Corporation (NASDAQ:TECH) announced today the launch of a portfolio of products and solutions that advance cancer research and enable the development and manufacturing of cell and gene therapies at the American Association for Cancer Research (AACR) Annual Meeting. The AACR Annual Meeting will be held in Orlando, Florida from April 14 to 19. At Bio-Techne's booth (#1763), a wide range of products, services, and technical capabilities will be showcased, including industry-leading reagents, immunoassays, proteomics analysis instruments, spatial biology solutions, and companion diagnostics for cancer research and exploration, the development of cell and gene therapies, and workflow solutions. Additionally, several of our scientists will present the latest information on therapeutic biomarkers, structural and chemical biology, and spatial biology through posters.

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Data Top Page

Advanced Western blotting solutions for regenerative medicine and cell therapy.

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This is a case study document on cell therapy and regenerative medicine using Simple Western (fully automated Western) and Single Cell Western. *Simple Western (Examples of quantifying proteins for the recovery of photoreceptors, treating autoimmune diseases like lupus, and multi-analysis of delaying hematopoietic stem cell aging) *Single Cell Western (Example of analyzing cardiomyocytes derived from stem cells)

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Exhibited at the 44th Annual Meeting of the Japanese Society for Molecular Biology.

  • Seminar・Event

We will be exhibiting at the 44th Annual Meeting of the Japanese Society for Molecular Biology, held from December 1 to 3 (Wednesday to Friday). Location: Inside the poster area, Booth A36 We will showcase equipment including Simple Western, and our group companies (ACD, Bio-Techne) will also be exhibiting. Additionally, if you visit our booth and show us the screen indicating that you have followed our company’s Twitter account on the spot, we will present you with original goods. We look forward to your visit.

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プロテインシンプルジャパン

プロテインシンプルジャパン

Pharmaceuticals and Biotechnology

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Protein Simple Japan Co., Ltd. is a member of a division based on Bio-Techne's protein-related products. We provide a wide range of tools, from immunoassay systems that quantify protein expression to systems that elucidate the structure and purity of protein-based pharmaceuticals. Please feel free to contact us if you have any inquiries. Contact email address: info.japan@proteinsimple.com X (formerly Twitter): https://x.com/ProteinSimpleJ

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