Human EpCAM positive exosome (CD9) ELISA kit
HAK-HELEPCAM09-1 Human EpCAM Positive Exosome ELISA Kit
Direct quantification of EpCAM-positive exosomes from blood samples and cell culture supernatants using the ELISA method!
This product is a two-step sandwich ELISA kit that utilizes high-performance monoclonal antibodies against CD9, an exosome marker, and EpCAM, which is said to be a marker for the diagnosis and prognosis of cancer derived from epithelial tissues, to directly and quantitatively detect EpCAM-positive exosomes secreted by cells in human blood and cell culture media.
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basic information
Component Products Anti-CD9 Antibody Solid Phase Plate 96 well 1 piece Standard Protein (EpCAM/CD9 Fusion Protein) 100µL 1 vial Assay Buffer 15mL 1 vial Washing Buffer (10×) 20µL 1 vial HRP-labeled Anti-PD-L1 Antibody (500×) 12mL 1 vial Substrate Solution 12mL 1 vial Stop Solution (2N H2SO4) 6mL 1 vial Plate Seal - 2 pieces
Price information
Suggested retail price: 100,000 yen
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Applications/Examples of results
Species origin: Human Application: Enzyme Linked Immunosorbent Assay Applicable samples: Serum, Plasma, Cell culture supernatant Measurement range (standard curve range): 0.313 to 20 ng/mL Sensitivity (standard substance): 0.14 ng/mL Purpose of use: This product is a two-step sandwich ELISA kit that utilizes high-performance monoclonal antibodies against CD9, an exosome marker, and EpCAM, which is said to be a marker for the diagnosis and prognosis of cancer derived from epithelial tissues, to directly quantify EpCAM-positive exosomes secreted by cells in human blood and cell culture media.
Detailed information
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This ELISA kit is based on a two-step sandwich method. The ELISA plate in the kit is pre-coated with anti-CD9 antibodies, and when a sample is added, the exosomes in the sample are trapped. After washing, an HRP-conjugated anti-EpCAM antibody is reacted with the EpCAM expressed on the trapped exosomes, and after adding the substrate, the color development by HRP is read and quantified using a plate reader.
Line up（3）
| Model number | overview |
|---|---|
| HAK-HELPDL109-1 | Human PD-L1 Positive Exosome (CD9) ELISA Kit |
| HAK-HEL0909-1 | CD9 Exosome ELISA Kit |
| HAK-HEL6363-1 | CD63 Exosome ELISA Kit |
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Company information
Hakarel Co., Ltd. primarily supports research on exosome detection methods and offers contract services such as the production of recombinant proteins, the creation of monoclonal antibodies, the establishment of ELISA measurement systems, exosome ELISA measurements, and proteomics analysis. We respond flexibly to customer requests and provide support for research and development. Please feel free to contact us if you have any inquiries.