We have found that it is an effective means to elucidate the relationship between hosts and parasites that is difficult to detect from appearance! *Case study collection is being distributed.
At the Kyoto University Center for Ecological Research, research is being conducted on the diversification of the genus Acer and the processes involved in coexistence among species. Our company has been commissioned to conduct part of the analysis, which we would like to introduce. In this analysis, we utilized host-specific blocking primers to suppress the amplification of host-derived genes, allowing for the efficient identification of the host, the leafroller moth, and a comprehensive exploration of parasites within it. We found that the analytical method using blocking primers is an effective means of elucidating the relationships between hosts and parasites that are difficult to detect visually. 【Case Overview】 ■ PCR was conducted in the presence and absence of blocking primers. ■ COI amplicon analysis was performed on 274 samples of leafroller moth larvae using Miseq. ■ From the obtained reads, we identified 10 species of leafroller moths and 13 species of parasitic wasps, clarifying the host-parasite relationships. *For more details, please refer to the PDF document or feel free to contact us.
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In recent years, advancements in molecular biology have led to remarkable progress in research such as the determination of the complete genome sequences of organisms and subsequent gene function analysis. Along with these technological advancements, molecular biological techniques such as nucleotide sequence analysis, real-time PCR, and DNA chips have become widely utilized in the field of food analysis. Since its establishment in 2001, Fasmak Co., Ltd. has been developing technologies for the "Japanese Standard Analysis Method" for genetically modified foods and food allergens in collaboration with related agencies of the Ministry of Agriculture, Forestry and Fisheries and the Ministry of Health, Labour and Welfare. The developed testing technologies are provided not only in Japan but also in the United States, China, and other countries. Additionally, Fasmak has been actively engaged in international standardization activities for "food inspection methods using molecular biological techniques" since its establishment, and its technical capabilities are internationally recognized. Furthermore, in partnership with Eurofins Scientific, one of the world's largest testing companies, Fasmak is advancing the introduction of the high technical capabilities possessed by the Eurofins Group. Moving forward, Fasmak will continue to provide world-class new testing technologies to everyone.
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