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  6. Measurement of reaction rate at 3000 s⁻¹ using a stopped-flow analysis system.

Measurement of reaction rate at 3000 s⁻¹ using a stopped-flow analysis system.

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SX20

last updated:Dec 25, 2023

テガサイエンス
テガサイエンス
  • Official site

A stopped-flow device optimal for steady-state and reaction kinetics studies!

The SX20 stop-flow device from Applied Photophysics is used to study the transient steady states of fast liquid-phase chemical and biochemical reactions initiated by the rapid mixing and stopping (stop-flow) of reactants. Spectroscopic probes such as absorbance and fluorescence are employed to observe the progress of the reaction by recording changes in the intensity of the spectral signal as a function of time. The general upper limit of reaction rates that can be measured using stop-flow spectroscopy is approximately 2000 s⁻¹. However, by utilizing the small volume cells adopted by the SX20, it becomes possible to measure reaction rates exceeding 3000 s⁻¹. *For more details, please download the PDF or feel free to contact us.*

    Other physicochemical equipment
SX20-1

Measurement of reaction rate at 3000 s⁻¹ using a stopped-flow analysis system.

SX20-1 550x550.png
SX20-1 550x550.png
  • Related Link - https://www.tegascience.co.jp/products/apl/sx20-su…

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basic information

**Main Features** - Extremely excellent micro-volume performance and outstanding sensitivity minimize sample consumption. - Capable of tracking reaction rates exceeding 3000 s⁻¹ with a dead time of less than 500 microseconds. - Optimized for absorbance and fluorescence applications without the need for system reconfiguration. - Powerful and user-friendly measurement, plotting, and analysis software. *For more details, please download the PDF or feel free to contact us.*

Price information

It varies depending on the composition. Please feel free to contact us.

Delivery Time

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※*It varies depending on the configuration. Please feel free to contact us.

Applications/Examples of results

Stop-flow measurements in biochemical research Examples: Enzyme mechanisms and redox reaction kinetics; protein folding

Detailed information

  • SX20-1

    **Overview** The SX20 is a clear market leader in the field of stopped-flow. Applied Photophysics supplies more than half of the stopped-flow analysis systems currently used worldwide, and these instruments have been used to produce over 2,000 academic papers to date.

  • sx_lamp

    [Light Source] The SX20 lamp house can be equipped with three types of lamps: an ozone-free 150W Xe lamp, an ozone-generating 150W Xe lamp, and an ozone-generating 150W Hg-Xe lamp. All lamps possess excellent stability and durability, meeting the stringent specifications set by Applied Photophysics.

  • sx-cell

    [Cell Design] The dimensions of the standard 20μL cell are 10mm x 2mm x 1mm, and two optical path lengths of 10mm and 2mm can be selected for use. The optical windows are masked with black quartz. The optical path length can be switched by changing the position of the photomultiplier detector and light guide, and the working time for this is approximately 1 minute.

catalog(9)

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News about this product(5)

【RX2000】

Reaction rate measurement is possible with existing spectrometers! Stop-flow measurement accessory RX2000 with a dead time of 8 milliseconds.

  • Product news

Combine with existing spectrophotometers to enable stopped-flow measurements! The RX2000 from Applied Photophysics is a high-performance stopped-flow measurement accessory. By combining it with existing UV-Vis spectrophotometers, fluorometers, and circular dichroism dispersers, it allows for the measurement of reactions that are over 1000 times faster compared to manual operations. With a dead time of just 8 milliseconds, the RX2000 can accommodate primary reaction rates exceeding 200 s^-1. ■ Adds stopped-flow measurement capabilities to existing UV-Vis spectrophotometers and fluorometers ■ Sample temperature control mechanism with a digital temperature display ■ Excellent dead time of 8 milliseconds allows for the measurement of reaction rates exceeding 200 s^-1 ■ Stable measurements enabled by gas pressure-driven accessories (optional) *Please feel free to contact us for more details.*

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【SX20】

For the study of high-speed reaction kinetics! Stop-flow device SX20 [500 microseconds]

  • Product news

The SX20 stop-flow device from Applied Photophysics is used to study the transient steady states of fast liquid-phase chemical and biochemical reactions initiated by the rapid mixing and stopping (stop-flow) of reactants. Spectroscopic probes such as absorbance and fluorescence are employed to observe the progress of the reaction by recording changes in the intensity of the spectral signal as a function of time. The general upper limit of reaction rates measurable by stop-flow spectroscopy is approximately 2000 s⁻¹. However, by utilizing the small volume cells adopted by the SX20, it becomes possible to measure reaction rates exceeding 3000 s⁻¹. 【Key Features】 ■ Minimizes sample consumption with extremely excellent micro-volume performance and outstanding sensitivity ■ Capable of tracking reaction rates exceeding 3000 s⁻¹ with a dead time of less than 500 microseconds ■ Optimized for absorbance and fluorescence applications without the need for system reconfiguration ■ Powerful and user-friendly measurement, plotting, and analysis software *Please feel free to contact us for more details.

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【Chirascan VX】

New standard for circular dichroism spectrometer!!! Circular dichroism spectrometer Chirascan VX【High sensitivity, low running cost】

  • Product news

Beyond α-helices and β-sheets! Detecting structural and thermodynamic properties! The Chirascan circular dichroism spectrometer from Applied Photophysics is suitable for the study of all types and sizes of chiral molecules and has been featured in thousands of publications since its launch in 2005. Chirascan data, commonly used for the analysis of biomolecules, helps deepen the understanding of the properties, mechanisms, and interactions of biomolecules. ■ Detect changes in secondary and tertiary structures ■ Detect responses to thermal or chemical changes ■ Study the mechanisms of folding and unfolding ■ Functionality expansion with accessories for Chirascan 【Key Features】 ■ High light throughput across the entire wavelength range ■ Obtain low-noise CD spectra in a short time ■ Measurement possible with a small sample amount ■ Reliable data through step-scan method and post-measurement smoothing processing ■ Low nitrogen gas consumption of 5L/min ■ Long lamp life *Please feel free to contact us for more details.

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【Chirascan V100】

Next-generation circular dichroism spectrometer!!! Circular dichroism spectrometer Chirascan V100 [Low sample consumption, high measurement speed]

  • Product news

The Chirascan V100 from Applied Photophysics is a circular dichroism (CD) spectrometer that can improve productivity and detection sensitivity for all CD applications. It is ideal for applications where samples and time are extremely valuable, such as in biopharmaceutical research or development departments. ■ High-sensitivity detector ■ Maximizes information from minimal samples ■ Dramatically reduces measurement time for sufficient CD application data acquisition ■ Simultaneous measurement of CD, absorbance, and fluorescence data ■ Absolute CD acquisition across the entire wavelength range through multi-point calibration ■ Opens the door to structural analysis of existing and unknown proteins 【Key Features】 ■ High-sensitivity solid-state detector ■ Quantitative CD measurement using DichOS ■ High light throughput across the entire wavelength range ■ Capable of obtaining low-noise CD spectra in a short time ■ Measurement possible with minimal sample amounts ■ Reliable data through step scan method and post-measurement smoothing processing ■ Low nitrogen gas consumption of 5L/min ■ Long lamp life *Please feel free to contact us for more details.*

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テガサイエンス

テガサイエンス

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Tegasaience Co., Ltd. is a specialized trading company for scientific and analytical instruments, headquartered in Kitakashiwa, Kashiwa City, near Lake Tega, where wild birds gather. Founded at the beginning of the 21st century, we have been providing excellent scientific and analytical instruments and precision equipment from Europe and the United States to domestic research institutions. Moving forward, we will continue to strive to contribute to the development of Japan's science and technology, focusing on areas that cannot be covered by domestic products. The bird in Tegasaience Co., Ltd.'s trademark is a colorful arrangement of the Eurasian Coot, which is abundant in Lake Tega.

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