It is an affinity chromatography resin that efficiently purifies the important T7 RNA polymerase used in the synthesis of mRNA drugs.
Cellufine Phosphate is an affinity chromatography resin that has an affinity for nucleic acid-binding proteins. T7 RNA polymerase can be efficiently purified using Cellufine Phosphate due to its nature as a nucleic acid-binding protein. T7 RNA polymerase is an RNA synthesis enzyme used to produce mRNA from DNA templates. In the manufacturing process of mRNA vaccines, which became famous as a vaccine for the novel coronavirus disease (COVID-19), T7 RNA polymerase is used as the enzyme for mRNA synthesis. T7 RNA polymerase can be purified to high purity through a two-step purification process using Cellufine Phosphate and Cellufine MAX DEAE (a weak anion exchange support). *For more details, please refer to the PDF document or feel free to contact us.
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**Features** - Base carrier: spherical cellulose particles - Ligand: phosphate ester group - Ligand concentration: 0.3 - 0.8 meq/ml - Protein adsorption capacity: ≧ 20 mg/ml-gel (lysozyme) It has affinity adsorption for nucleic acid-binding proteins such as RNA polymerase. Since the phosphate group is negatively charged, it does not adsorb negatively charged impurities such as endotoxins. For stationary cleaning, 0.2M sodium hydroxide can be used. Therefore, it can be reused multiple times. *For more details, please refer to the PDF document or feel free to contact us.*
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For more details, please refer to the PDF document or feel free to contact us.
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Our group was founded in 1906 as Sogi Electric Co., Ltd., and since then, we have been a pioneer in Japan's chemical industry, contributing to the development of society by expanding renewable energy generated by hydropower into the field of electrochemistry and developing domestic nitrogen fertilizers. Our unchanging mission is to engage in manufacturing that harmonizes with the global environment, to solve our customers' challenges in order to realize a rich life, and to cultivate outstanding human resources who can thrive globally, continuously serving society.