Selection of antibodies that bind only to mutant types! An article introducing the production of antibodies that distinguish between mutant types differing by just one amino acid.
In cases where a protein with a single amino acid mutation exists in a mixed state alongside the wild-type sequence, there may be a need for antibodies that can specifically distinguish this mutated protein. Specifically, as an antigen, a peptide synthesized from the mutated sequence portion is used, and through panning via phage display and ELISA screening, clones that are negative for the wild-type and positive for the peptide with the single amino acid mutation are selected. I will introduce each step. [Contents] 1. Design and coupling of peptide antigens 2. Panning 3. ELISA screening 4. Sequencing 5. Selection of candidate antibodies *For detailed content of the article, please refer to the related links. *For detailed specifications, please refer to the PDF materials or feel free to contact us.
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*You can view the detailed content of the article through the related link. *For detailed specifications, please refer to the PDF document or feel free to contact us.
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Gene Frontier has been expanding its business areas that contribute to the acceleration of biopharmaceuticals, including antibody drugs, and has been advancing the development of its unique technologies. Moving forward, we aim to accelerate our research and development and business development to achieve further growth on a global scale. We will continue to provide unique technologies and services for the development of innovative pharmaceuticals and diagnostic drugs, striving to be a trusted partner for our customers and contribute to 21st-century healthcare.