Introduction to Custom Monoclonal Antibody Production Service Case Study Vol. 7

Setting the antigens is very important! An article introducing the selection of antibodies that distinguish the differences at the terminal ends.

When proteins undergo processing and are divided into multiple fragments, it becomes very important to set the antigens for producing antibodies that recognize each fragment. Antibody artisans perform all selections in vitro without using animal immunization, allowing them to conduct binding reactions with peptides or proteins in their prepared state, without the steps of fragmenting proteins for antigen presentation. In this article, we will introduce the antigen setting and selection methods for producing antibodies that can distinguish between fragments before and after cleavage by a protease of a certain protein. 【Contents】 ■1. Specificity of antibodies ■2. Design of antigens ■3. Screening methods 　・3-1. Panning 　・3-2. ELISA screening ■4. Protein antigens and peptide antigens 　・4-1. Which one should be chosen? 　・4-2. Innovations in selection ■5. Summary *For detailed content of the article, please refer to the related links. *For detailed specifications, please refer to the PDF materials or feel free to contact us.

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