Using the desalting tube "Solnac Tube" for LC/MS! Removing TFA and conducting negative ion measurement.
When analyzing acidic compounds using reversed-phase partition chromatography, an acidic mobile phase (set to a pH more than 2 units lower than the pKa of the analyte) may be used to suppress dissociation. Common acidic mobile phases suitable for use in LC/MS include acetic acid, formic acid, and trifluoroacetic acid (TFA), with TFA being appropriate for use at low pH. However, TFA is considered to have too high an acidity, which can lead to ionization suppression with almost 100% probability when detecting the analyte as a negative ion. TFA was removed from the eluent using a Solnac tube, and negative ion measurements were conducted. For details on conditions and analysis results, please download the catalog. 【LC Conditions (Partial)】 ■ Equipment: Agilent 1200 ■ Flow rate: 0.3 ml/min ■ Detector: UV (210 nm) ■ Sample: Tryptophan 20 ppm solution ■ Injection volume: 5 μL *For more details, please refer to the PDF document or feel free to contact us.
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【MS Conditions (Partial)】 ■Device: JEOL JMS-T100LP ■Ionization Method: ESI Neg. ■Needle Voltage: 2000 V ■Orifice 1 Voltage: 50 V ■Desolvation Chamber Temperature: 250 ℃ *For more details, please refer to the PDF document or feel free to contact us.
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For more details, please refer to the PDF document or feel free to contact us.
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Our company develops contract services utilizing our unique high-level technical expertise in imaging mass spectrometry and liquid chromatography mass spectrometry. As a venture originating from Hamamatsu University School of Medicine, we leverage our unique technology related to IMS, cultivated over many years in the Cell Molecular Anatomy course, along with our extensive experience in mass spectrometry, including a Level 5 LC/MS analyst certification, to provide high-quality mass spectrometry data.