The binding with the object can be adjusted by the structural change caused by the irradiation light wavelength
Dihydrofolate reductase (DHFR) is known as a target of the anticancer drug MTX, and research tools (kits for evaluating protein subcellular localization, etc.) utilizing the binding between Escherichia coli DHFR (eDHFR) and MTX or its analogues have been reported and sold. The inventors developed azoMTX, in which an azo group is introduced into MTX, and found that the binding to eDHFR by light irradiation can be regulated. They also found that the kinetics of eDHFR fusion protein expressed in cells can be manipulated by light using azoMTX derivatives. 【effect】 ・The structure of azoMTX is reversibly changed by light irradiation of a specific wavelength. The cis of azoMTX produced under UV irradiation strongly bound to eDHFR and inhibited its enzymatic activity, while the trans of azoMTX produced under visible light irradiation decreased the binding strength to eDHFR and the enzymatic reaction proceeded. ・The target protein was expressed as a fusion protein with eDHFR, and intracellular kinetics (localization) could be reversibly photomanipulated using an azoMTX derivative.
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The revenue generated from technology transfer is reinvested as new research funding for universities and researchers, and is utilized to create further research outcomes. To ensure the smooth operation of this cycle, known as the "Intellectual Creation Cycle," we will vigorously promote technology transfer. The types of seeds we handle include patents, know-how, databases, and programs. We have established a collaborative framework by signing basic technology transfer agreements with the following universities (as of June 1, 2025): Tohoku University, Hirosaki University, Iwate University, Akita University, Fukushima University, Yamagata University, Tohoku Gakuin University, Iwate Medical University, Fukushima Medical University, Aizu University, Miyagi University, Hokkaido University, Muroran Institute of Technology, and Showa Medical University.
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