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  6. What are the types of separation modes in HPLC? Free explanatory materials available.

What are the types of separation modes in HPLC? Free explanatory materials available.

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last updated:Oct 11, 2024

アジレント・テクノロジー株式会社
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What are the characteristics of each separation mode in HPLC? A comprehensive and easy-to-understand explanation of the basics of HPLC! Free basic knowledge materials are currently available.

In chromatography, the differences in the affinity of substances for the stationary phase and the mobile phase are utilized. The separation modes commonly used in HPLC can be broadly classified into adsorption, partition, and size exclusion based on the type of affinity. The main separation modes include "adsorption," "partition," "hydrophilic interaction," "ion exchange," and "size exclusion." This document provides a clear summary of the fundamentals of liquid chromatography (LC), detectors, and principles. It is recommended for those who are learning HPLC for the first time, those who want to review from the basics, and those looking for materials useful for in-house training. [Contents (partial)] ■ Introduction ■ HPLC Equipment ■ Separation in HPLC ■ Detection *For more details, please download the PDF or feel free to contact us.

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What are the types of separation modes in HPLC? Free explanatory materials available.

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【Other Published Content】 ■Peak Identification and Quantification in HPLC ■Terminology of Chromatography ■History of Agilent LC ■Agilent InfinityLab LC System/Solution Portfolio *For more details, please download the PDF or feel free to contact us.

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[Information] Basics of LC

[Information] Basics of LC

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Introducing the next-generation HPLC that assists users and promotes automation and digital transformation in pharmaceuticals, chemicals, food, and the environment.

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By utilizing HPLC equipped with smart features that assist daily operations, it contributes to high productivity and a reduction in human errors. This enables the establishment of a sustainable and comfortable laboratory.

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アジレント・テクノロジー株式会社

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Agilent was born out of the Hewlett-Packard Company's spin-off in 1999. Since then, we have received numerous awards related to innovation and have obtained patents for inventions that surpass those accolades. More importantly, the number of customers we serve has been increasing year by year, demonstrating the strength of our partnerships. Agilent has become a leader in the life sciences, diagnostics, and applied chemistry markets. We provide instruments, services, consumables, applications, and expertise to laboratories around the world, contributing to the discovery of insights that our customers are exploring. Through our expertise and reliable collaboration, we offer highly dependable solutions to our customers.

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