Induction of differentiation from iPS cells to NKT cells

Introduction of results on the differentiation induction from iPS cells to hematopoietic stem progenitor cells.

We conducted experiments to promote the differentiation of iPS cells into hematopoietic stem progenitor cells (HSPC) using "PG-007," "PG-008," and "PG-010," which can be utilized in the differentiation induction process into NKT cells. We compared the productivity, differentiation induction efficiency of HSPC, as well as the productivity, differentiation induction efficiency, and functionality of NKT cells, with control conditions using existing methods such as VEGF, CHIR99021, and TPO. As a result, the differentiation induction process was shortened by one day, and more than twice the number of HSPC cells was obtained compared to existing methods. [Summary of Results] ■ "PG-007" demonstrated sufficient effect at one-tenth the concentration of VEGF, "PG-008" at one 2500th the concentration of CHIR99021, and "PG-010" at one-fifth the concentration of TPO. ■ The differentiation efficiency into NKT cells and the functionality of the obtained NKT cells were equivalent to the control conditions, while the number of NKT cells obtained significantly increased. *For more detailed test results, please download the materials from the link below.

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