Outsourced intestinal environment analysis [organic acids (short-chain fatty acids), microbiota, stool collection kit]

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last updated：Nov 07, 2025

テクノスルガ・ラボ

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Analysis of organic acids (short-chain fatty acids) in feces and analysis of bacterial and fungal flora (microbiome) are possible. A fecal collection kit that allows for storage of collected feces at room temperature is also available for sale.

One method for evaluating the functionality of food is the analysis of the intestinal environment, which has garnered attention. For example, by examining organic acids (short-chain fatty acids) in feces and the intestinal microbiota, changes in the intestinal environment can be captured. Our company offers analysis services for organic acids (short-chain fatty acids) in feces and intestinal microbiota. In intestinal environment analysis, feces are often used as a non-invasive sample that can be collected. It is recommended that feces be quickly frozen using liquid nitrogen or similar methods after collection and stored at -80°C. Maintaining this frozen state is particularly one of the hurdles in sample collection for intestinal environment analysis involving humans. Therefore, our company has developed and sells a collection kit that allows for the preservation of short-chain fatty acids and intestinal microbiota without freezing the feces. [For more details, please check the related links.]

Related Link - https://www.tecsrg.co.jp/techinfo/gut-environment/

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basic information

■ Analysis of Organic Acids (Short-Chain Fatty Acids) in Feces ● Analysis Method 　LC (pH-buffered post-column conductivity detection method) ● Analysis Items 　Acetic acid, propionic acid, succinic acid, lactic acid, iso-butyric acid, iso-valeric acid, n-butyric acid, n-valeric acid, formic acid ■ Microbiome Analysis ● Amplicon Sequencing Analysis 　◎ Analysis of the microbiome in feces 　◎ Analysis at the genus to species level 　〈Analysis Items: Identification of reads, α and β diversity analysis using QIIME2, statistical analysis using R, predictive metagenomic analysis, etc.〉 ■ Fecal Collection Kit "Metabolokeeper" ● Can stably maintain organic acids (short-chain fatty acids) and microbiome in feces at room temperature for about one month ● Quantitative concentration correction is possible using internal standard substances in the preservation solution

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Applications/Examples of results

Measurement of changes in intestinal environment during functional food intake Evaluation of the health status and balance of gut microbiota Assessment of intestinal barrier function Monitoring the therapeutic effects on obesity and diabetes Research on the relationship between gut microbiota and metabolites Research on the correlation between specific diseases and intestinal environment, etc.

Detailed information

【Metabolic Keeper (Feces) Collection Kit】 This kit allows for intestinal microbiota analysis and fecal physicochemical analysis without freezing. The upper part of the container is filled with a preservation solution, which enables quantitative concentration correction using internal standard substances in the preservation solution. The available fecal physicochemical analysis items include the analysis of organic acids in feces. This kit is sold in combination with the Rakuryu Cup WIDE.
The accuracy of the data obtained from analysis and research outsourcing is extremely important. Our intestinal environment analysis has been utilized by many researchers for over 20 years. Furthermore, the results have already been used in numerous academic papers.