Technical Data: Metabolomics Analysis Using High-Resolution GC-MS
A deeper exploration of the metabolome! A comprehensive metabolomics approach from target analysis to the identification of unknown compounds.
This technical document introduces a workflow for metabolomics analysis using the Orbitrap GC-MS system and Compound Discoverer software. Data acquisition through EI full scan with a mass resolution of 60,000 and data extraction and deconvolution analysis with Compound Discoverer accurately extracts peaks characteristic of the sample. It also includes statistical analysis functions to identify differences between sample groups, designed to allow visual confirmation of results. Furthermore, the workflow for identifying unknown compounds using precise mass information from EI, PCI, and MS/MS quickly lists candidate compounds by comparing mass accuracy within a few ppm, PCI's adduct ion information, and in silico MS/MS fragment information. Features: - The Orbitrap GC-MS HRAM metabolomics library includes information on over 800 metabolites and Kovats retention time information. - Compatible with commercially available integer mass libraries. - Reliable compound identification using precise mass information from EI, PCI, and MS/MS. - Detection of component differences through multivariate statistical analysis and pathway analysis.
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**Product Specifications** Thermo Scientific Orbitrap Exploris GC / Orbitrap Exploris GC 240 Mass Spectrometer ● Maximum resolution 30,000 – 240,000 (FWHM) @ m/z 200 ● Mass accuracy < 1 ppm ● System sensitivity < 6 fg OFN ● Thermo Scientific NeverVent technology allows for switching between EI/CI, ion source maintenance, and column replacement without breaking vacuum Thermo Scientific Compound Discoverer Software ● An integrated platform for low molecular weight qualitative analysis using high-resolution mass spectrometry ● Flexible customization of analysis workflows according to objectives ● Peak detection algorithm that minimizes false positives ● Molecular formula calculation for detected components, database search for MS and MSn spectra ● Exploration of metabolites and related compounds based on MSn spectra ● Statistical analysis features to assist in interpreting large data sets *For more details, please refer to the PDF document or feel free to contact us.*
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【Usage】 ■Identification of metabolites For research use only. Not for diagnostic purposes. *For more details, please refer to the PDF materials or feel free to contact us.
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Thermo Fisher Scientific K.K., the Japanese subsidiary of Thermo Fisher Scientific Incorporated, is a sales company for comprehensive system analysis instruments, bio-related equipment, and research reagents. In Japan, it has bases in Yokohama, Tokyo, Osaka, Fukuoka, and Nagoya, handling various analytical instruments including mass spectrometers, various bio-related equipment, measuring instruments, medical devices, lab software, and research reagents.