The non-denaturing analysis of antibody-drug conjugates (ADCs) is settled with this!
TSKgel UP-SW3000-LS / TSKgel HIC-ADC Series
<Column demonstration in progress> Analyzing aggregate and drug-antibody ratio (DAR) using SEC column and HIC column.
As analytical methods for antibody-drug conjugates (ADCs), size exclusion chromatography (SEC) and hydrophobic interaction chromatography (HIC), which can be analyzed in a non-denaturing state, are important. Therefore, the analysis of aggregates by SEC and the analysis of drug-antibody ratio (DAR) by HIC are conducted as a set of evaluation methods. ■ SEC Analysis Column: [TSKgel UP-SW3000-LS] TSKgel UP-SW3000-LS is a column with low protein adsorption. It is suitable for the separation of antibody (IgG) aggregates, dimers, monomers, and fragments. ■ HIC Analysis Columns: [TSKgel HIC-ADC Butyl / TSKgel HIC-ADC Phenyl] The TSKgel HIC-ADC series has a higher separation capability for ADCs compared to the existing TSKgel Butyl-NPR and is an effective column for DAR analysis. We offer two types of functional groups with different separation selectivity for samples: butyl and phenyl groups. There are 10 cm columns for high-resolution analysis and 3.5 cm columns for fast analysis. To consider the durability of the columns, guard columns are also available.
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basic information
■SEC Analysis Column 【TSKgel UP-SW3000-LS】 Particle Size: 2 µm Pore Size: 25 nm Column Size: 4.6 mm I.D. × 15 cm or 4.6 mm I.D. × 30 cm Molecular Weight Fractionation Range: 10 to 500 kDa ■HIC Analysis Column 【TSKgel HIC-ADC Butyl / TSKgel HIC-ADC Phenyl】 Ligand: Butyl Group, Phenyl Group Particle Size: 5 µm Pore Size: Non-porous Column Size: 4.6 mm I.D. × 3.5 cm or 4.6 mm I.D. × 10 cm
Price information
■SEC Analysis Column 【TSKgel UP-SW3000-LS】 4.6 mm I.D. × 15 cm: 230,000 yen 4.6 mm I.D. × 30 cm: 320,000 yen ■HIC Analysis Column 【TSKgel HIC-ADC Butyl / TSKgel HIC-ADC Phenyl】 4.6 mm I.D. × 3.5 cm: 110,000 yen 4.6 mm I.D. × 10 cm: 220,000 yen *For details, please contact your distributor.*
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Applications/Examples of results
■ SEC Analysis Column 【TSKgel UP-SW3000-LS】 - Proteins (antibodies, enzymes) - Protein aggregates, fragments - Purity verification, rapid analysis, quality control ■ HIC Analysis Column 【TSKgel HIC-ADC Butyl / TSKgel HIC-ADC Phenyl】 - DAR analysis of ADCs, purity analysis - Analysis of antibody drugs, biopharmaceuticals, protein aggregates, fragments, isomer separation and analysis - Stereoisomer separation and analysis of plasmid DNA We also offer demo services for the columns. Please feel free to contact us.
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Company information
The BioScience Division is engaged in business in the "Measurement Field," which includes liquid chromatography, columns, and separation agents, and the "Diagnostics Field," which focuses on immunodiagnostics, glycohemoglobin analysis, and genetic testing. In each field, we are expanding our product lineup based on distinctive technologies and establishing sales bases not only in Japan but also overseas for global business development. Additionally, the BioScience Division consistently handles everything from the development, manufacturing, and sales of equipment and reagents to maintenance and customer support, all managed by the Tosoh Group, including its affiliated companies.