東ソー Company Profile

During the production of bispecific antibodies (BsAb), various impurities such as mispaired antibodies, aggregates, incomplete antibodies, and fragment antibodies are often generated. Therefore, in the downstream purification process of BsAb, it is important to effectively separate and remove these impurities derived from various products and process-related impurities using chromatography. This report presents the separation modes of chromatography used in the isolation and purification of BsAb, along with the corresponding packing materials and separation conditions.

Anion exchange chromatography (AEC) is often used for the separation and purification of oligonucleotides. However, modified oligonucleotide complexes tend to be highly hydrophobic, which can lead to the formation of aggregates and higher-order structures, resulting in poor separation and decreased recovery rates. Numerous reports have been made on solutions to this issue, including investigations into separation conditions such as elution buffer composition. This summary compiles information on the elution buffers used in chromatography separations, such as AEC separation of modified oligonucleotide complexes.

We have published a data collection of tests and analyses of herbal medicines using TSKgel. This data collection introduces analysis examples for 20 items among the herbal medicines listed in the eighteenth revised edition of the Japanese Pharmacopoeia. 【Analysis subjects included】 1. … Onsei-in extract 2. … Ougon 3. … Kouren-ketokutou extract 4. … Kakkontou extract 5. … Kamikihitou extract 6. … Kanzo 7. … Keishibukuryougan extract 8. … Kouboku 9. … Gojyuuyutou extract 10. … Goleisan extract 11. … Saiko 12. … Saiko-keishitou extract 13. … Sanshishi 14. … Shakuyaku 15. … Ninjin 16. … Hanjaku-houbakutou extract 17. … Byakko-ka-ninjin-tou extract 18. … Botanki 19. … Rokkunzitou extract 20. … Reikeijutsugantou extract * A printed version is also available. If you would like one, we can send it by mail. Please contact us via "Inquiries" to request this.