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Analysis Product List

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Genotyping Analysis: PCR-NGS Analysis

Genotyping Analysis: PCR-NGS Analysis

Detailed analysis of mosaic mutations! Introducing genotyping analysis using Illumina MiSeq.

At Fasmac, we offer "PCR-NGS analysis." We conduct detailed analysis of the types and frequencies of mutations using next-generation sequencers. The analysis device used is the "Illumina MiSeq," which allows for the simultaneous analysis of one target site (up to 16 samples). For details regarding pricing, delivery times, and more, please feel free to contact us. 【Analysis Details】 ■ Simultaneous analysis of 16 samples for one target site ■ Example: Knockout in cultured cells ■ Analysis device: Illumina MiSeq *For more information, please refer to the PDF document or feel free to contact us.

  • Contract Analysis
  • Contract Analysis
  • Analysis Services

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Genotyping Analysis: PCR-Sanger Sequencing Analysis

Genotyping Analysis: PCR-Sanger Sequencing Analysis

Detection of small indels and single nucleotide substitutions! Introducing our genotyping analysis.

At Fasmac, we offer "PCR-Sanger Seq analysis." With Sanger sequencing, we can confirm the presence of single nucleotide substitutions and the number of nucleotide deletions/inserts that cannot be detected by electrophoresis analysis. Samples can include tissue fragments from mice, rats, zebrafish, and medaka, as well as cultured cell pellets, and the deliverables will be sequencing analysis data. Please feel free to contact us when you need our services. 【Analysis Details】 ■ Delivery Time: 1-2 weeks ■ Samples: Tissue fragments from mice, rats, zebrafish, and medaka, cultured cell pellets, etc. ■ Deliverables: Sequencing analysis data ■ Analysis Equipment: Applied Biosystems 3730xl DNA Analyzer *For more details, please refer to the PDF document or feel free to contact us.

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  • Contract Analysis
  • Analysis Services

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Next-generation sequencing analysis "Microbial Genome Draft Analysis Package"

Next-generation sequencing analysis "Microbial Genome Draft Analysis Package"

Samples can accept bacteria and archaea! *Case study collection is currently being distributed.

The "Microbial Genome Draft Analysis Package" is a service that can be used reasonably by being packaged. Acceptable samples include bacteria and archaea. As optional services, we offer auto-annotation using Prokka, BLAST analysis of contigs, and sequence type typing. Please feel free to contact us when you need our services. 【Analysis Overview】 (1) Customer: Send the extracted genomic DNA to us. (2) Our company: Perform QC work on the received samples. (3) Our company: Prepare the adapter library for MiSeq analysis. (4) Our company: Conduct a 2×300 bp run on MiSeq. (5) Our company: Assemble the acquired data and create contigs. *For more details, please refer to the PDF document or feel free to contact us.

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Genotyping analysis

Genotyping analysis

PCR-electrophoresis analysis and PCR-NGS analysis! Detection using real-time PCR and digital PCR is also possible.

To everyone who is worried about 'having to do genotyping but not having enough time,' leave the labor-intensive tasks to Fasmac. In our 'Genotyping Analysis,' we perform DNA extraction, PCR, and mutation detection. Please send us tissue samples from mice or zebrafish, cultured cell pellets, etc. We will provide a quote based on the number of samples and the analysis you require. Additionally, detection using real-time PCR and digital PCR is also possible. 【Line-up】 ■ PCR-Electrophoresis Analysis: Detection of larger indels and screening using HMA ■ PCR-Sanger Seq Analysis: Detection of small indels and single nucleotide substitutions ■ PCR-NGS Analysis: Detailed analysis of mosaic mutations *For more details, please refer to the PDF document or feel free to contact us.

  • Contract Analysis
  • Contract Analysis
  • Analysis Services

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Next-generation sequencing analysis "Targeted amplicon analysis"

Next-generation sequencing analysis "Targeted amplicon analysis"

We offer a variety of optional services! Data is visualized as "microbial community composition graphs" and "heat maps." *Case study collection available.

"Target Amplicon Analysis" is a service that can be used reasonably by packaging it. The target regions for analysis include 16S V4, 18S V9, ITS (gITS7-ITS4), COI (mlCOIintF-HCO2198), and MiFish (12S V5), among others. We provide primers for sample preparation targeting the amplification regions free of charge, and you will send us your samples. After that, we will use QIIME and other tools to visualize the data as "microbial community composition graphs" and "heat maps." [Optional Services (Excerpt)] - Change of target region length (approximately 400-500 bp) - Library mix preparation - Rarefaction curve and principal coordinate analysis (PCoA) - OTU BLAST analysis *For more details, please refer to the PDF document or feel free to contact us.

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