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Next-generation sequencing analysis "Microbial Genome Draft Analysis Package"

Samples can accept bacteria and archaea! *Case study collection is currently being distributed.

The "Microbial Genome Draft Analysis Package" is a service that can be used reasonably by being packaged. Acceptable samples include bacteria and archaea. As optional services, we offer auto-annotation using Prokka, BLAST analysis of contigs, and sequence type typing. Please feel free to contact us when you need our services. 【Analysis Overview】 (1) Customer: Send the extracted genomic DNA to us. (2) Our company: Perform QC work on the received samples. (3) Our company: Prepare the adapter library for MiSeq analysis. (4) Our company: Conduct a 2×300 bp run on MiSeq. (5) Our company: Assemble the acquired data and create contigs. *For more details, please refer to the PDF document or feel free to contact us.

  • Contract Analysis

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Technical Information Magazine 201905-01 Crystal Orientation Analysis with High Spatial Resolution

The technical information magazine The TRC News provides the latest information on analytical techniques that are useful for research and development, solving production troubles, and quality control.

**Abstract** Using the TEM-based crystal orientation analysis system "ASTAR," it is possible to achieve higher spatial resolution than that of SEM-based EBSD (with spatial resolution of various EBSD methods being around tens of nanometers, while the ACOM-TEM method using ASTAR achieves 2 to 5 nm). Additionally, it is characterized by the ability to identify a greater variety of crystal structures. By obtaining crystal orientation maps, crystal phase maps, and grain size distributions that are difficult to acquire through conventional TEM analysis, quantitative interpretation becomes possible. Furthermore, since measurements can be taken in the same field of view as TEM observations, it allows for complex analyses combined with (S)TEM-EDX/EELS and the use of in-situ TEM. **Table of Contents** 1. Introduction 2. ACOM-TEM Method Using ASTAR 3. Examples of Analysis Using ASTAR 4. Conclusion

  • Contract Analysis
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miRNA Contract Analysis Service "Premium 700"

Utilizing patented technology, a service for absolute quantification of 700 types of miRNA with excellent "specificity," "detection sensitivity," and "reproducibility."

HMT has partnered with MiRXES, based in Singapore, to provide miRNA (microRNA) analysis services. The ID3EAL technology, which utilizes MiRXES's patented technology, is an excellent miRNA measurement method with superior specificity, detection sensitivity, and reproducibility, supporting your research and development. One of our contract analysis services, "Premium 700," is a contract analysis service that performs absolute quantification of 700 types of miRNA using ID3EAL technology. It is recommended for clinical trials and biomarker discovery. *For more details, please refer to the link below or feel free to contact us.*

  • Public Testing/Laboratory

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miRNA contract analysis service "PanoramiR"

Utilizing patented technology, miRNA measurement with excellent "specificity," "detection sensitivity," and "reproducibility." An optimal service for biomarker discovery and disease mechanism analysis.

HMT has partnered with MiRXES, a company based in Singapore, to provide miRNA (microRNA) analysis services. The ID3EAL technology, which utilizes MiRXES's patented technology, offers an excellent method for miRNA measurement with high specificity, detection sensitivity, and reproducibility, supporting your research and development. One of our contract analysis services, the 'PanoramiR miRNA KNOWLEDGE PANEL,' is a contract analysis service that uses a profiling panel of 376 carefully selected miRNAs related to various diseases. The target miRNAs are selected based on databases such as miRbase 22, HMDD v3.2, The Cancer Genome Atlas, as well as our own research and influential academic papers. This service is recommended for basic research in biomarker discovery and disease mechanism analysis. *For more details, please refer to the link below or feel free to contact us.*

  • Public Testing/Laboratory

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Contracted analysis service for gene expression "NET-CAGE"

The NET-CAGE analysis that inputs the "NET-RNA" currently being synthesized. New service starting in the fall of 2019! An evolved version of CAGE analysis.

In the conventional "CAGE" analysis, total RNA is input, allowing for the following: - RNA expression analysis (serving the same role as general RNA-seq) - Identification of transcription start sites (the greatest strength of CAGE, which is insufficient with RNA-seq) - Searching for transcription factor binding motifs based on the identified transcription start site data (unique to CAGE, a reverse search function of ChIP-seq) The central principle of library preparation is the "cap trap method," a unique technology developed by RIKEN that captures the cap structure at the 5' end of RNA. In this "NET-CAGE" analysis, "NET-RNA," which is currently being synthesized in the nucleus, is extracted and recovered using a unique technique and input for library preparation. (NET-RNA: Negative Elongating-Transcript: newly synthesized RNA) Generally, the lifespan of total RNA is relatively long, ranging from several hours to several days. In contrast, the eRNA (enhancer RNA) from NET-CAGE has an extremely short lifespan, being degraded within just a few minutes. "CAGE" allows us to observe the total RNA present in the cell, while "NET-CAGE" enables us to see the NET-RNA that is currently being synthesized. It has become possible to differentiate between the points we want to observe.

  • Other Nucleic Acid/DNA Research
  • Other bio-related products and services
  • Other cell research

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Technical Information Magazine 201901-02 Nanometer Scale Local Structure Analysis

The technical information magazine The TRC News provides the latest information on analytical techniques that are useful for research and development, solving production troubles, and quality control.

**Abstract** Spectroscopic analysis techniques utilizing near-field light localized at the tips of metal chips are highly anticipated methods for analyzing chemical structures in the nanometer range. Techniques such as tip-enhanced Raman spectroscopy and near-field Raman spectroscopy are representative methods, and various studies have been conducted on their principles and applications. This paper presents examples of their application in material analysis and discusses their practicality for material characterization. **Table of Contents** 1. Introduction 2. Crystal structure analysis of CNTs using TERS 3. Stress analysis at the SiO2/SiC interface using SNOM-Raman 4. Conclusion

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Technical Information Magazine 202001-02 Crystal Structure Analysis of Gallium Oxide

The technical information magazine The TRC News provides the latest information on analytical techniques that are useful for research and development, solving production troubles, and quality control.

**Abstract** Gallium oxide (Ga2O3) is attracting attention as a next-generation power semiconductor material, and research has been actively conducted in recent years. Optimizing process technology is essential for improving the reliability and characteristics of semiconductor devices, making the evaluation methods crucial. This paper presents examples of crystal structure analysis necessary for assessing the quality of epitaxial films, as well as the analysis of impurities, defects, and carrier concentration in the ion implantation process, which significantly impacts device characteristics. **Table of Contents** 1. Introduction 2. Crystal structure analysis using cross-sectional TEM and planar STEM 3. Evaluation of the ion implantation process 4. Conclusion

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Technical Information Magazine 201904-02 Cellulose Nanofiber Structural Analysis

The technical information magazine The TRC News provides the latest information on analytical techniques that are useful for research and development, solving production troubles, and quality control.

**Abstract** In the research and development of CNF, the use of electron microscopy for observation has become a necessary evaluation across almost all material fields and research phases. Particularly for observing CNF dispersed in polymers using TEM, it is essential to employ the technique of "electron staining," which has been crucial for preparing TEM samples of polymer materials. In this presentation, we will introduce examples of morphological observations of composite materials using CNF, as well as observation cases of the CNF itself, structural analysis examples such as crystallinity measured by 13C solid-state NMR, and examples of compositional sugar analysis using acid hydrolysis HPLC-fluorescence detection. **Table of Contents** 1. Introduction 2. Overview of Morphological Observation 3. Observation Cases of CNF and CNF/Polymer 4. Evaluation of CNF Crystallinity 5. Compositional Sugar Analysis of CNF (HPLC-Fluorescence Detection) 6. Conclusion

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Technical Information Magazine 202303-01 Comprehensive Analysis of Biomolecular Interactions

This paper presents a comprehensive analysis of interactions using multiple methods, including surface plasmon resonance and isothermal titration calorimetry.

The technical information magazine The TRC News provides the latest information on analytical techniques that are useful for research and development, solving production troubles, and quality control. [Abstract] Molecular interaction analysis is an indispensable method in the study of life phenomena and the development of therapeutic drugs. The information on interactions obtained by different methods varies; for instance, surface plasmon resonance provides kinetic parameters of interactions, while isothermal titration calorimetry can acquire thermodynamic parameters of interactions. This paper introduces comprehensive analysis cases of interactions using multiple methods through multifaceted analysis. [Table of Contents] 1. Introduction 2. Surface Plasmon Resonance / Isothermal Titration Calorimetry 3. Analysis of the Interaction between STING and cGAMP 4. Conclusion

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