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Column "AdvanceBio MS Spent Media"

Column "AdvanceBio MS Spent Media"

Rapid MS-based workflow! No need for sample derivatization, saving time and resources.

The "AdvanceBio MS Spent Media" is a HILIC column that can separate non-derivatized amino acids and other polar metabolites in cell culture media with high speed, excellent sensitivity, and reproducibility, making it suitable for mass spectrometry. By combining it with Agilent InfinityLab LC series instruments and Agilent MS instruments, a comprehensive solution for cell culture component analysis is achieved. The AdvanceBio MS Spent Media analysis is an innovative solution designed for the generation and characterization of biomolecules, as a new product in the Agilent AdvanceBio family. 【Features】 ■ Achieves excellent peak shape and recovery rates even for challenging ionic metabolites ■ Baseline chromatographic separation capability for isomers of leucine and isoleucine ■ Columns tested with amino acids to ensure quality and performance ■ Excellent analytical sensitivity with a mobile phase suitable for MS ■ Compatible with both HPLC and UHPLC systems *For more details, please refer to the PDF document or feel free to contact us.

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Column "AdvanceBio RP-mAb"

Column "AdvanceBio RP-mAb"

It can be analyzed in a shorter time compared to a column filled with all porous particles of the same size!

"AdvanceBio RP-mAb" is a column designed to optimize the performance of intact and reduced mAb analysis for the discovery, development, and QA/QC applications of biopharmaceuticals. Based on proprietary technology related to Poroshell technology and pore size and bonding phases, it provides accurate and reproducible analytical results with higher separation capability and faster analysis times. It accommodates various chemical properties such as SB-C8, C4, and diphenyl. 【Features】 ■ Enhances mAb separation capability with surface porous particles (3.5μm) of a wide pore size (450Å), while maintaining compatibility with all LC equipment. ■ Prevents inlet clogging and extends column life with robust Poroshell packing material and 2μ inlet frit. ■ Available in bonding phases of SB-C8, C4, and diphenyl. *For more details, please refer to the PDF document or feel free to contact us.

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Column "AdvanceBio Deionization RP Cartridge"

Column "AdvanceBio Deionization RP Cartridge"

Time-saving through online sample desalting! Concentrate the sample before elution.

The "AdvanceBio Desalting RP Cartridge" is a column that can efficiently and rapidly remove salt ions online before MS detection. It can be used in combination with any LC system to desalinate collected fractions. Additionally, it can be used with the Agilent 1290 Infinity II 2D-LC system, performing desalting on the second dimension after separation on the first dimension. 【Features】 ■ Reduced MS downtime and maintenance ■ Improved data quality and reliability of results ■ Large pore size of 1000 Å suitable for biopharmaceuticals ■ Stability from pH 2 to 13 ■ Compatible with commonly used reverse phase mobile phases *For more details, please refer to the PDF document or feel free to contact us.

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Agilent BioSEC-5 LC Column for Biopharmaceutical Analysis

Agilent BioSEC-5 LC Column for Biopharmaceutical Analysis

Achieving high reproducibility and long lifespan size-based separation for biomolecules!

The "Agilent BioSEC-5" is an LC column for biopharmaceutical analysis, filled with 5μm silica particles coated with a unique neutral hydrophilic layer to maximize column efficiency and stability. The specially designed packing increases pore volume, enhancing both peak capacity and resolution. To accommodate a wide range of size exclusion analyses, pore sizes of 100Å, 150Å, 300Å, 500Å, 1000Å, and 2000Å are available. 【Features】 ■ High recovery rates for a wide range of size-based biomolecule separations ■ Superior reproducibility and column lifespan compared to common products ■ Excellent stability under high pH, high salt, and low salt conditions ■ Usable with most aqueous buffers *For more details, please refer to the PDF document or feel free to contact us.

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Agilent BioMAb LC Column for Biopharmaceutical Analysis

Agilent BioMAb LC Column for Biopharmaceutical Analysis

Particles, membranes, and bonds are resistant under high pressure, achieving higher resolution and faster separation!

The "Agilent BioMAb" is an LC column for biopharmaceutical analysis that uses a unique resin designed for high-resolution ion exchange separation of monoclonal antibodies. It is available in particle sizes of 1.7μm, 3μm, 5μm, and 10μm, with smaller particle size columns offering higher resolution. The packing material consists of rigid spherical cross-linked polystyrene-divinylbenzene (PS/DVB) non-porous beads. 【Features】 ■ Particles grafted with a hydrophilic polymer layer that virtually eliminate non-specific binding of antibody proteins. ■ A weak cation exchange (WCX) layer that is chemically bonded to a hydrophilic polymer coating, extremely uniform and densely packed. *For more details, please refer to the PDF document or feel free to contact us.

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Column "ZORBAX 300Extend-C18"

Column "ZORBAX 300Extend-C18"

High robustness and separation of polypeptides and peptides over a wide range of pH from high pH to low pH (pH 2 to 11.5)!

The "ZORBAX 300Extend-C18" is a wide-bore HPLC column that efficiently separates peptides in the pH range of 2 to 11.5. Its unique bidentate bonding phase provides excellent longevity and reproducibility in both high and low pH regions. The retention characteristics and selectivity for peptides and polypeptides in the high pH region significantly change as these molecules become charged. 【Features】 ■ Shows different selectivity at high and low pH ■ Achieves excellent efficiency and good recovery rates for hydrophobic peptides at high pH ■ Suitable for LC/MS analysis using ammonium hydroxide mobile phase *For more details, please refer to the PDF document or feel free to contact us.

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Column "ZORBAX RRHD 300 HILIC"

Column "ZORBAX RRHD 300 HILIC"

The HILIC function is specially designed for the separation of glycopeptide variants!

The "ZORBAX RRHD 300 HILIC" is a column for the analysis of glycopeptides, featuring a particle size of 1.8μm and a pore size of 300Å. It is designed to provide consistent and outstanding performance for the separation and characterization of peptides, proteins, antibodies, complexes, novel biomaterials, and biopharmaceuticals. The technology of AdvanceBio columns enhances accuracy and productivity, allowing for more information to be obtained in various characterization analyses. 【Features】 ■ Excellent peak shape for polar glycopeptides - These polar compounds typically cannot be separated using reverse phase columns. ■ High-speed analysis and stability up to 1200 bar - With a particle size of 1.8μm, it enables fast separation using UHPLC without compromising data accuracy. *For more details, please refer to the PDF document or feel free to contact us.

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Column "ZORBAX RRHD - Wide Pore"

Column "ZORBAX RRHD - Wide Pore"

Achieving excellent separation capability with 1.8μm particles in most defined separations!

The "ZORBAX RRHD-Wide Pore" is an LC column suitable for the analysis of biopharmaceuticals, providing highly reproducible separations of proteins and peptides. To efficiently separate proteins, peptides, or other macromolecules, the sample needs to be in close proximity to the stationary phase surface, which is why such a wide pore (300Å) column is necessary. Four different stationary phases (C18/C8/C3/Diphenyl) are available, allowing for the optimization of selectivity and recovery rates for proteins and polypeptides. 【Features】 ■ High-pressure (1200 bar) column for obtaining suitable results with the 1290 Infinity LC or other UHPLC systems ■ Usable with HILIC Plus ■ The selectivity remains the same for 3.5μm and 5μm ZORBAX columns with the same stationary phase, ensuring compatibility with various LCs *For more details, please refer to the PDF document or feel free to contact us.

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Gel filtration column "ZORBAX GF-250/450"

Gel filtration column "ZORBAX GF-250/450"

A highly efficient, robust, and reproducible product for protein separation up to 3 mL/min!

The "ZORBAX GF-250/450" is a gel filtration column suitable for size separation of proteins and other biomolecules. When using the GF-250 and GF-450 columns in tandem, the separation range for spherical proteins is from 4,000 to 900,000. By using a hydrophilic diol bonded phase, it demonstrates a high protein recovery rate (typically >90%) and is compatible with a wide pH range of pH 3 to 8 due to its unique zirconia-modified silica. 【Features】 ■ Compatible with organic modifiers (<25%) and denaturants in the mobile phase ■ Capable of removing protein aggregates to accurately determine protein size ■ Common analytical applications include separation of monomers, dimers, and aggregates, desalting, estimation of protein molecular weight, and separation of modified proteins *For more details, please refer to the PDF document or feel free to contact us.

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Fast Guard Column for Bioanalysis

Fast Guard Column for Bioanalysis

Available for use with AdvanceBio Glycan Mapping Columns (2.7μm, surface porous, pressure resistant up to 600 bar)!

We would like to introduce the "Fast Guard Column for Bioanalysis" that we handle. The "Agilent Bio UHPLC Guard" is a high-performance guard column designed for Agilent's high-speed LC column family, using easy-to-install hardware that fits directly at the end of the column. By using the Fast Guard for UHPLC, you can extend the lifespan of the analytical column without compromising performance. 【Features】 - No special hardware required, just connect to the column - Comes in a package of three - Usable with AdvanceBio Peptide Mapping Columns (pressure tolerance 600 bar) - Usable with AdvanceBio Glycan Mapping Columns (1.8μm, pressure tolerance 1200 bar) - Extends the lifespan of high-speed LC analytical columns without degrading performance *For more details, please refer to the related links or feel free to contact us.

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Capillary column/nano column

Capillary column/nano column

Compatible with various LC/MS interfaces! Available inner diameters of 0.5mm, 0.3mm, 0.1mm, and 0.075mm.

We would like to introduce our LC columns for bio-pharmaceutical analysis, 'Capillary Columns/Nano Columns.' You can choose from a wide variety of stationary phases, pore sizes, and dimensions. These columns are suitable for applications where the sample amount is extremely limited, as they enhance sensitivity by minimizing sample dilution within the column. 【Features】 ■ Extremely high sensitivity when the sample amount is very limited ■ Compatible with various LC/MS interfaces ■ Available inner diameters: 0.5mm, 0.3mm, 0.1mm, and 0.075mm ■ Stationary phases can accommodate a wide range of molecular weights, from small to large ■ Suitable for one-dimensional (1-D) and two-dimensional (2-D, proteomics) applications *For more details, please refer to the related links or feel free to contact us.

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mRP-C18 High Recovery Protein Column

mRP-C18 High Recovery Protein Column

Silica filled with porous C18 bonded ultra-pure 5μm particles that reduce or remove protein adsorption!

We would like to introduce our bio-pharmaceutical analysis LC column, the 'mRP-C18 High Recovery Protein Column'. This column and the desalting LC column can separate complex protein samples (e.g., immunodepleted serum or plasma proteins) into the number of fractions selected by the user, as well as perform desalting of the samples. It achieves significantly better recovery rates for protein samples compared to conventional RP HPLC columns. Please feel free to consult us if you have any requests. 【Features】 ■ Load up to a total of 380μg of protein ■ Available in two column sizes (0.5×100mm, 4.6×50mm) ■ Maximum operating pressure is 250bar (4000psi) ■ Compatible with water and all common organic solvents ■ Confirmed 95-99% protein sample recovery rate using immunodepleted serum with a multiple affinity removal system LC column *For more details, please refer to the related links or feel free to contact us.

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HAS/IgG Multiple Affinity Removal Column

HAS/IgG Multiple Affinity Removal Column

Removal of albumin and IgG! This improves the sensitivity of subsequent sample LC/MS analysis and electrophoresis.

We would like to introduce the "HAS/IgG Multiple Affinity Removal Column" that we handle. The column format is "4.6 x 50mm," and custom-made column sizes and spin cartridges are also available. By using the multiple affinity removal system, approximately 69% of total proteins (albumin and IgG) can be removed. With an optimized two-buffer system, the column can be reused with very high reproducibility for over 200 injections, and it is provided with a convenient column reagent starter kit. 【Features】 ■ The serum injection volume per analysis is 100μL for a column with an inner diameter of 4.6mm. ■ The protocol and column are optimized for the analysis of human biological samples. ■ Compatible with most FPLC or HPLC pumps and equipment, including Agilent 1200 instruments. ■ Human serum albumin/IgG affinity removal spin cartridges are also available. ■ Up to 20,000μL of plasma/serum can be processed until the column's lifespan is reached (for a column with an inner diameter of 4.6mm). *For more details, please refer to the related links or feel free to contact us.

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JP18 Pharmaceutical Test Item LC Analysis Relevant Column

JP18 Pharmaceutical Test Item LC Analysis Relevant Column

Introduction of each pharmaceutical item! In addition to the specified columns, test items and others are also included.

On our website, we introduce HPLC columns that comply with the 18th edition of the Japanese Pharmacopoeia (Notification No. 220 by the Ministry of Health, Labour and Welfare, dated June 7, 2021). We have listed pharmaceuticals such as "Actinomycin D," "Aclarubicin," "Acrinol Hydrate," and "Azathioprine" by each item. Additionally, if there are no catalog products for the corresponding structure of fillers or column sizes, we indicate "No applicable products." There is a possibility that we can propose custom columns, so please contact us if you have any requests. [Items Listed (Partial)] ■ Actinomycin D ■ Aclarubicin ■ Acrinol Hydrate ■ Azathioprine ■ Acyclovir *For more details, please refer to the related links or feel free to contact us.

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Bond Elut Sodium Sulfate dehydration column

Bond Elut Sodium Sulfate dehydration column

The cartridge filled with ACS grade granular anhydrous sodium sulfate allows for an easy drying process using sodium sulfate.

The cartridge filled with ACS grade granular anhydrous sodium sulfate allows for an easy drying process using sodium sulfate. We offer three types of cartridges (LRC, Bond Elut Jr, straight barrel). *For more details, please refer to the PDF document or feel free to contact us.*

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