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Column "ZORBAX RRHD 300 HILIC"

The HILIC function is specially designed for the separation of glycopeptide variants!

The "ZORBAX RRHD 300 HILIC" is a column for the analysis of glycopeptides, featuring a particle size of 1.8μm and a pore size of 300Å. It is designed to provide consistent and outstanding performance for the separation and characterization of peptides, proteins, antibodies, complexes, novel biomaterials, and biopharmaceuticals. The technology of AdvanceBio columns enhances accuracy and productivity, allowing for more information to be obtained in various characterization analyses. 【Features】 ■ Excellent peak shape for polar glycopeptides - These polar compounds typically cannot be separated using reverse phase columns. ■ High-speed analysis and stability up to 1200 bar - With a particle size of 1.8μm, it enables fast separation using UHPLC without compromising data accuracy. *For more details, please refer to the PDF document or feel free to contact us.

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Column "ZORBAX RRHD - Wide Pore"

Achieving excellent separation capability with 1.8μm particles in most defined separations!

The "ZORBAX RRHD-Wide Pore" is an LC column suitable for the analysis of biopharmaceuticals, providing highly reproducible separations of proteins and peptides. To efficiently separate proteins, peptides, or other macromolecules, the sample needs to be in close proximity to the stationary phase surface, which is why such a wide pore (300Å) column is necessary. Four different stationary phases (C18/C8/C3/Diphenyl) are available, allowing for the optimization of selectivity and recovery rates for proteins and polypeptides. 【Features】 ■ High-pressure (1200 bar) column for obtaining suitable results with the 1290 Infinity LC or other UHPLC systems ■ Usable with HILIC Plus ■ The selectivity remains the same for 3.5μm and 5μm ZORBAX columns with the same stationary phase, ensuring compatibility with various LCs *For more details, please refer to the PDF document or feel free to contact us.

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Gel filtration column "ZORBAX GF-250/450"

A highly efficient, robust, and reproducible product for protein separation up to 3 mL/min!

The "ZORBAX GF-250/450" is a gel filtration column suitable for size separation of proteins and other biomolecules. When using the GF-250 and GF-450 columns in tandem, the separation range for spherical proteins is from 4,000 to 900,000. By using a hydrophilic diol bonded phase, it demonstrates a high protein recovery rate (typically >90%) and is compatible with a wide pH range of pH 3 to 8 due to its unique zirconia-modified silica. 【Features】 ■ Compatible with organic modifiers (<25%) and denaturants in the mobile phase ■ Capable of removing protein aggregates to accurately determine protein size ■ Common analytical applications include separation of monomers, dimers, and aggregates, desalting, estimation of protein molecular weight, and separation of modified proteins *For more details, please refer to the PDF document or feel free to contact us.

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ZORBAX Bio-SCX Series ii

LC and LC/MS columns! It is possible to achieve high retention and excellent peak shape.

The "ZORBAX Bio-SCX Series II" is a column designed for optimal 2D separation of peptides and proteins using LC/MS. The packing is based on 3.5μm ultra-high purity ZORBAX silica particles, combined with a polymer that is functionalized with sulfonic acid groups, taking into account environmental considerations. As a result, high retention and excellent peak shape can be achieved in the ion exchange procedures for 2D analysis of peptides and proteins. 【Features】 ■ Available in column configurations of 35mm length × 0.3mm inner diameter and 50mm length × 0.8mm inner diameter ■ pH range: 2.5 to 8.5 *For more details, please refer to the related links or feel free to contact us.

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Fast Guard Column for Bioanalysis

Available for use with AdvanceBio Glycan Mapping Columns (2.7μm, surface porous, pressure resistant up to 600 bar)!

We would like to introduce the "Fast Guard Column for Bioanalysis" that we handle. The "Agilent Bio UHPLC Guard" is a high-performance guard column designed for Agilent's high-speed LC column family, using easy-to-install hardware that fits directly at the end of the column. By using the Fast Guard for UHPLC, you can extend the lifespan of the analytical column without compromising performance. 【Features】 - No special hardware required, just connect to the column - Comes in a package of three - Usable with AdvanceBio Peptide Mapping Columns (pressure tolerance 600 bar) - Usable with AdvanceBio Glycan Mapping Columns (1.8μm, pressure tolerance 1200 bar) - Extends the lifespan of high-speed LC analytical columns without degrading performance *For more details, please refer to the related links or feel free to contact us.

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Capillary column/nano column

Compatible with various LC/MS interfaces! Available inner diameters of 0.5mm, 0.3mm, 0.1mm, and 0.075mm.

We would like to introduce our LC columns for bio-pharmaceutical analysis, 'Capillary Columns/Nano Columns.' You can choose from a wide variety of stationary phases, pore sizes, and dimensions. These columns are suitable for applications where the sample amount is extremely limited, as they enhance sensitivity by minimizing sample dilution within the column. 【Features】 ■ Extremely high sensitivity when the sample amount is very limited ■ Compatible with various LC/MS interfaces ■ Available inner diameters: 0.5mm, 0.3mm, 0.1mm, and 0.075mm ■ Stationary phases can accommodate a wide range of molecular weights, from small to large ■ Suitable for one-dimensional (1-D) and two-dimensional (2-D, proteomics) applications *For more details, please refer to the related links or feel free to contact us.

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Biomonolith <Protein A, Protein G>

Used primarily for the quantification of IgG (calculation of fermentation potency)!

We would like to introduce our bio-pharmaceutical analysis LC column, 'Bio Monolith <Protein A, Protein G>'. It enables high-speed and accurate quantification of mAb antibody titers and allows for the purification of small amounts of mAb for further characterization. It can be used with HPLC and UHPLC systems, including Agilent 1100, 1200, and 1260 Bio Inert Quaternary LC. The Bio Monolith Protein G column is suitable for the purification of IgG molecules that do not bind to Bio Monolith Protein A. 【Features】 ■ Separation analysis of all IgG subclasses (human and mouse) ■ Protein G provides different selectivity for IgG molecules that do not bind to Protein A ■ Separation unaffected by flow rate ■ High-speed movement between mobile and stationary phases ■ Fixation of method parameters ■ Significant reduction in analysis time and buffer usage *For more details, please refer to the related links or feel free to contact us.

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mRP-C18 High Recovery Protein Column

Silica filled with porous C18 bonded ultra-pure 5μm particles that reduce or remove protein adsorption!

We would like to introduce our bio-pharmaceutical analysis LC column, the 'mRP-C18 High Recovery Protein Column'. This column and the desalting LC column can separate complex protein samples (e.g., immunodepleted serum or plasma proteins) into the number of fractions selected by the user, as well as perform desalting of the samples. It achieves significantly better recovery rates for protein samples compared to conventional RP HPLC columns. Please feel free to consult us if you have any requests. 【Features】 ■ Load up to a total of 380μg of protein ■ Available in two column sizes (0.5×100mm, 4.6×50mm) ■ Maximum operating pressure is 250bar (4000psi) ■ Compatible with water and all common organic solvents ■ Confirmed 95-99% protein sample recovery rate using immunodepleted serum with a multiple affinity removal system LC column *For more details, please refer to the related links or feel free to contact us.

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HAS/IgG Multiple Affinity Removal Column

Removal of albumin and IgG! This improves the sensitivity of subsequent sample LC/MS analysis and electrophoresis.

We would like to introduce the "HAS/IgG Multiple Affinity Removal Column" that we handle. The column format is "4.6 x 50mm," and custom-made column sizes and spin cartridges are also available. By using the multiple affinity removal system, approximately 69% of total proteins (albumin and IgG) can be removed. With an optimized two-buffer system, the column can be reused with very high reproducibility for over 200 injections, and it is provided with a convenient column reagent starter kit. 【Features】 ■ The serum injection volume per analysis is 100μL for a column with an inner diameter of 4.6mm. ■ The protocol and column are optimized for the analysis of human biological samples. ■ Compatible with most FPLC or HPLC pumps and equipment, including Agilent 1200 instruments. ■ Human serum albumin/IgG affinity removal spin cartridges are also available. ■ Up to 20,000μL of plasma/serum can be processed until the column's lifespan is reached (for a column with an inner diameter of 4.6mm). *For more details, please refer to the related links or feel free to contact us.

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JP18 Pharmaceutical Test Item LC Analysis Relevant Column

Introduction of each pharmaceutical item! In addition to the specified columns, test items and others are also included.

On our website, we introduce HPLC columns that comply with the 18th edition of the Japanese Pharmacopoeia (Notification No. 220 by the Ministry of Health, Labour and Welfare, dated June 7, 2021). We have listed pharmaceuticals such as "Actinomycin D," "Aclarubicin," "Acrinol Hydrate," and "Azathioprine" by each item. Additionally, if there are no catalog products for the corresponding structure of fillers or column sizes, we indicate "No applicable products." There is a possibility that we can propose custom columns, so please contact us if you have any requests. [Items Listed (Partial)] ■ Actinomycin D ■ Aclarubicin ■ Acrinol Hydrate ■ Azathioprine ■ Acyclovir *For more details, please refer to the related links or feel free to contact us.

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Plgel Olexis

Optimization for the analysis of high molecular weight polymers and high-temperature GPC analysis.

The "PLgel Olexis GPC column" is optimized for the analysis of high molecular weight polymers and high-temperature GPC analysis. It has become the industry standard for the analysis of polyolefins, polyethylene, polypropylene, and high-performance polymers. Even at high temperatures, this product achieves excellent efficiency, long lifespan, and high resolution without the risk of shear degradation. The column is compatible with green solvents such as 1,2,4-trichlorobenzene (TCB), dichlorobenzene (DCB), chloronaphthalene, and butyl. *For more details, please refer to the PDF materials or feel free to contact us.*

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PL Aquagel-OH SEC fractionation

Rapid scale-up of water-based size exclusion and gel filtration chromatography as expected.

By using the "PL aquagel-OH fractionation column," you can quickly scale up aqueous size exclusion and gel filtration chromatography as expected. The column is packed with analytical-grade 8µm packing material. Compared to common packing materials with larger particle sizes and broader particle size distributions, the analysis speed and resolution are significantly improved. This excellent resolution allows for the accurate fractionation of high-purity target components with a high recovery rate in less than 10 minutes of analysis time per column. *For more details, please refer to the PDF document or feel free to contact us.

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PL HFIPgel

Enabling GPC of solvent-resistant polymers at room temperature! A column with an inner diameter of 4.6mm is also available.

"PL HFIPgel" is a product developed to exhibit excellent performance with hexafluoroisopropanol (HFIP) and related polar fluorinated solvents. It analyzes nylon, polyamide, polyester, PLLA, and PLGA at room temperature, eliminating unnecessary distortion, positional shifts, shoulder peaks, and low resolution in calibration curves caused by HFIP. Additionally, it achieves high performance without unnatural results due to its suitable separation range, and we also offer a column with an inner diameter of 4.6 mm to reduce solvent consumption. [Features] - Analyzes nylon, polyamide, polyester, PLLA, and PLGA at room temperature - Provides reliable results with HFIP and fluorinated solvents - Eliminates unnecessary distortion, positional shifts, shoulder peaks, and low resolution in calibration curves caused by HFIP *For more details, please refer to the PDF document or feel free to contact us.

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PL Multisolvent

Adopting silica with excellent efficiency and robustness! Eliminating the expansion and contraction of pores due to changes in solvents.

"PL Multisolvent" is a product that coats silica with a large pore volume using an innovative process involving inert polymers. The column separates low to medium molecular weight polymers in solvents such as water and tetrahydrofuran (THF) without secondary reactions. The silica backbone provides excellent resolution and eliminates degradation due to expansion during solvent transfer. 【Features】 ■ Utilizes silica with excellent efficiency and robustness ■ The silica backbone with high strength eliminates pore expansion and contraction caused by solvent changes ■ Supports high-resolution separation of polymers, additives, oligomers, and plasticizers up to 100,000 Da *For more details, please refer to the PDF document or feel free to contact us.

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Bond Elut Plexa

Simple sample processing steps! Designed to obtain high-precision and highly reliable analytical results.

"Bond Elut Plexa" is a product that allows for the acquisition of clean and pure extracts with high recovery rates and in a short time, using a simple sample processing step. Its innovative surface with hydroxyl groups and groundbreaking polymer structure are designed to provide more accurate and reliable analytical results without compromising the performance of analytical instruments. Additionally, regardless of the analytical field or sample matrix, it enables easy and fast processing with high fractionation performance, making validation straightforward. 【Features】 ■ 45μm spherical styrene-divinylbenzene (SDVB) polymer ■ Average pore size: 150Å ■ Retention mechanism: Hydrophobic interaction ■ Packing amounts: 30mg, 60mg, 200mg, 500mg, 10g ■ Column sizes: 1mL, 3mL, 6mL, 60mL ■ Column shape: Straight *For more details, please refer to the PDF document or feel free to contact us.

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