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カラム『AdvanceBio オリゴヌクレオチド』

独自のテクノロジーを使用!高pH移動相に対する耐性を大幅に高めている

『AdvanceBio オリゴヌクレオチド』は、高効率、2.7μmの表面多孔質 Poroshell粒子が特長のバイオ医薬品分析用LCカラムです。 エンドキャップ処理を施したC18相で オリゴヌクレオチドの選択性を高めます。 さらに、分離能標準を用いて当製品をバッチテストすることで、 一貫した性能を実現しています。 【特長】 ■HPLCおよびUHPLCシステムの長寿命カラムによる、  オリゴヌクレオチドの高分解能分離 ■結果の信頼性の向上 ・Poroshellの効率的な粒子形態により高分解能分離を実現 ■コストの削減 ・高pHに対して優れた耐性を持つ堅牢な化学修飾シリカによりカラムを長寿命化 ※詳しくはPDF資料をご覧いただくか、お気軽にお問い合わせ下さい。

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"Poroshell 300" LC column for biopharmaceutical analysis

In addition to protein separation for analysis, it can also be used for LC/MS separation with ammonia-containing mobile phases!

The "Poroshell 300" is an ideal LC column for the analysis of biopharmaceuticals, designed for the rapid separation of proteins and peptides. This column, featuring unique particles, maintains sharp and efficient peak shapes even at high flow rates. With a unique particle structure that combines a porous silica surface thin phase and a hard silica core, it shortens the diffusion distance of proteins, enabling practical high-speed HPLC separations for peptides and proteins up to 500–1,000 kDa. 【Features】 ■ The Poroshell 300 column, bonded with StableBond phases (C18, C8, C3), exhibits excellent stability and sensitivity in TFA and formic acid mobile phases. ■ Usable at pH 2 to 11, it achieves unique separations and high selectivity. *For more details, please refer to the PDF document or feel free to contact us.

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HAS/IgG Multiple Affinity Removal Column

Removal of albumin and IgG! This improves the sensitivity of subsequent sample LC/MS analysis and electrophoresis.

We would like to introduce the "HAS/IgG Multiple Affinity Removal Column" that we handle. The column format is "4.6 x 50mm," and custom-made column sizes and spin cartridges are also available. By using the multiple affinity removal system, approximately 69% of total proteins (albumin and IgG) can be removed. With an optimized two-buffer system, the column can be reused with very high reproducibility for over 200 injections, and it is provided with a convenient column reagent starter kit. 【Features】 ■ The serum injection volume per analysis is 100μL for a column with an inner diameter of 4.6mm. ■ The protocol and column are optimized for the analysis of human biological samples. ■ Compatible with most FPLC or HPLC pumps and equipment, including Agilent 1200 instruments. ■ Human serum albumin/IgG affinity removal spin cartridges are also available. ■ Up to 20,000μL of plasma/serum can be processed until the column's lifespan is reached (for a column with an inner diameter of 4.6mm). *For more details, please refer to the related links or feel free to contact us.

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JP18 Pharmaceutical Test Item LC Analysis Relevant Column

Introduction of each pharmaceutical item! In addition to the specified columns, test items and others are also included.

On our website, we introduce HPLC columns that comply with the 18th edition of the Japanese Pharmacopoeia (Notification No. 220 by the Ministry of Health, Labour and Welfare, dated June 7, 2021). We have listed pharmaceuticals such as "Actinomycin D," "Aclarubicin," "Acrinol Hydrate," and "Azathioprine" by each item. Additionally, if there are no catalog products for the corresponding structure of fillers or column sizes, we indicate "No applicable products." There is a possibility that we can propose custom columns, so please contact us if you have any requests. [Items Listed (Partial)] ■ Actinomycin D ■ Aclarubicin ■ Acrinol Hydrate ■ Azathioprine ■ Acyclovir *For more details, please refer to the related links or feel free to contact us.

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Column "AdvanceBio MS Spent Media"

Rapid MS-based workflow! No need for sample derivatization, saving time and resources.

The "AdvanceBio MS Spent Media" is a HILIC column that can separate non-derivatized amino acids and other polar metabolites in cell culture media with high speed, excellent sensitivity, and reproducibility, making it suitable for mass spectrometry. By combining it with Agilent InfinityLab LC series instruments and Agilent MS instruments, a comprehensive solution for cell culture component analysis is achieved. The AdvanceBio MS Spent Media analysis is an innovative solution designed for the generation and characterization of biomolecules, as a new product in the Agilent AdvanceBio family. 【Features】 ■ Achieves excellent peak shape and recovery rates even for challenging ionic metabolites ■ Baseline chromatographic separation capability for isomers of leucine and isoleucine ■ Columns tested with amino acids to ensure quality and performance ■ Excellent analytical sensitivity with a mobile phase suitable for MS ■ Compatible with both HPLC and UHPLC systems *For more details, please refer to the PDF document or feel free to contact us.

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Column "AdvanceBio Amino Acid Analysis (AAA)"

Designed as an innovative solution for the characterization of biomolecules!

The "AdvanceBio Amino Acid Analysis (AAA)" is an LC column for biopharmaceutical analysis that can separate amino acids from protein hydrolysates and cell culture media with high speed, excellent sensitivity, and reproducibility. It includes proven reagents for amino acid derivatization, a prepared amino acid standard kit, columns based on our innovative Poroshell technology, and support from our experts. When used in conjunction with Agilent InfinityLab LC series instruments, it provides a complete solution for amino acid analysis. 【Features】 ■ Achieves high separation capability with the efficient particle morphology of Poroshell ■ Long column life due to robust chemically modified silica with excellent resistance to high pH ■ Utilizes 2.7μm diameter particles, compatible with both HPLC and UHPLC systems ■ Standard solutions and reagents available as a kit ■ Uses a general analysis injection system *For more details, please refer to the PDF document or feel free to contact us.

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InfinityLab Poroshell 120 Column

Method development is quick and easy! Compatible with almost all LC systems, with 12 types of fillers available.

The "InfinityLab Poroshell 120 Column" enhances analytical efficiency and reliability, significantly improving the performance of various instruments. Whether you desire superior HPLC performance, want to achieve UHPLC performance at lower pressures, or are seeking the highest level of UHPLC performance, this column is suitable for you. Available in three particle sizes: 1.9μm, 2.7μm, and 4μm, allowing you to choose the appropriate particle size based on your separation needs. 【Features】 ■ A column with innovative chiral stationary phase on porous surface particles ■ High-speed, high-separation column that increases throughput and separation capability ■ Excellent inter-batch reproducibility that consistently delivers reliable separation results ■ Selectable particle sizes Particle sizes of 1.9μm, 2.7μm, and 4μm maximize the performance of methods and instruments *For more details, please refer to the PDF document or feel free to contact us.

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Multiple Affinity Removal LC Column Human-6

Remove six types of abundant proteins with a simple method.

The "Multiple Affinity Removal Column Human6" is an LC column designed for biopharmaceutical analysis that removes six types of proteins, including abundant albumin, from human plasma samples, making it easier to separate and identify low-concentration proteins in human serum. By removing high-concentration proteins, the dynamic range of analysis can be effectively expanded. It can enhance the performance of LC/MS and electrophoresis analysis of serum samples. 【Features】 - Removes approximately 85-90% of total protein - Available in three column sizes: - 4.6 x 50mm - 4.6 x 100mm - 10 x 100mm *For more details, please refer to the related links or feel free to contact us.

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Multiple Affinity Removal Column - Human7

Remove seven types of excess protein with a simple method.

The "Multiple Affinity Removal Column Human7" is an LC column designed for biopharmaceutical analysis that removes seven types of proteins, including abundant albumin, from human plasma samples, making it easier to separate and identify low-concentration proteins in human serum. By removing high-concentration proteins, the dynamic range of analysis can be effectively expanded. It can enhance the performance of LC/MS and electrophoresis analysis of serum samples. 【Features】 ■ Removes approximately 88-92% of total protein ■ Available in three column sizes: ・4.6 x 50mm ・4.6 x 100mm ・10 x 100mm ■ Custom-made column sizes are also available *For more details, please refer to the related links or feel free to contact us.

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Multiple Affinity Removal Column Mouse3

Remove three types of excess protein with a simple method.

The "Agilent Mouse3 Multiple Affinity Removal Column" is designed to remove three types of proteins, including the abundant albumin, from mouse plasma samples, making it easier to separate and identify low-concentration proteins in mouse serum. By removing high-concentration proteins, the dynamic range of analysis can be effectively expanded, enhancing the performance of LC/MS and electrophoresis analyses of serum samples. (This product is not intended for use other than research purposes.) *For more details, please refer to the related links or feel free to contact us.

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ZORBAX column / Poroshell column

Dramatic improvement in productivity! Flexibility and scalability of methods for low molecular weight and biomolecule analysis.

The "ZORBAX Columns / Poroshell Columns" are specially designed products for HPLC analysis with high productivity. The silica used is of extremely high purity, very robust, and highly uniform, providing the highest level of reliability. Additionally, one of the features is the extremely wide range of phases and column configurations that cater to diverse application needs. 【Features of ZORBAX Columns】 ■ Excellent options and flexibility for low molecular weight and reversed-phase biomolecule analysis with over 18 phases, including Eclipse Plus and HILIC ■ Easy method development that demonstrates excellent performance across a wide range of target compounds and conditions ■ Improvement of UHPLC methods ■ Outstanding peak shape performance due to proven innovative silica and bonding technology ■ Long-term reliability by having the necessary options available when needed for analysis and preparative purification *For more details, please refer to the PDF materials or feel free to contact us.

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mRP-C18 High Recovery Protein Column

Silica filled with porous C18 bonded ultra-pure 5μm particles that reduce or remove protein adsorption!

We would like to introduce our bio-pharmaceutical analysis LC column, the 'mRP-C18 High Recovery Protein Column'. This column and the desalting LC column can separate complex protein samples (e.g., immunodepleted serum or plasma proteins) into the number of fractions selected by the user, as well as perform desalting of the samples. It achieves significantly better recovery rates for protein samples compared to conventional RP HPLC columns. Please feel free to consult us if you have any requests. 【Features】 ■ Load up to a total of 380μg of protein ■ Available in two column sizes (0.5×100mm, 4.6×50mm) ■ Maximum operating pressure is 250bar (4000psi) ■ Compatible with water and all common organic solvents ■ Confirmed 95-99% protein sample recovery rate using immunodepleted serum with a multiple affinity removal system LC column *For more details, please refer to the related links or feel free to contact us.

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UHPLC column

Equivalent selectivity to existing ZORBAX columns! Optimize separation by changing the stationary phase!

This product is a UHPLC column that achieves a maximum operating pressure of 120 MPa. When combined with the Agilent 1290 Infinity LC System, it delivers the highest level of performance. It is suitable for continuous analysis with durability and stable reproducibility, making it compatible with high-pressure UHPLC systems from various manufacturers. 【Features】 ■ Achieves a maximum operating pressure of 120 MPa through optimization of hardware and packing processes ■ Delivers the highest level of performance when combined with the Agilent 1290 Infinity LC System ■ Equivalent selectivity to existing ZORBAX columns ■ Durability and stable reproducibility suitable for continuous analysis ■ Compatible with high-pressure UHPLC systems from various manufacturers *For more details, please refer to the PDF document or feel free to contact us.

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Fast Guard Column for Bioanalysis

Available for use with AdvanceBio Glycan Mapping Columns (2.7μm, surface porous, pressure resistant up to 600 bar)!

We would like to introduce the "Fast Guard Column for Bioanalysis" that we handle. The "Agilent Bio UHPLC Guard" is a high-performance guard column designed for Agilent's high-speed LC column family, using easy-to-install hardware that fits directly at the end of the column. By using the Fast Guard for UHPLC, you can extend the lifespan of the analytical column without compromising performance. 【Features】 - No special hardware required, just connect to the column - Comes in a package of three - Usable with AdvanceBio Peptide Mapping Columns (pressure tolerance 600 bar) - Usable with AdvanceBio Glycan Mapping Columns (1.8μm, pressure tolerance 1200 bar) - Extends the lifespan of high-speed LC analytical columns without degrading performance *For more details, please refer to the related links or feel free to contact us.

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Column "ZORBAX RRHD - Wide Pore"

Achieving excellent separation capability with 1.8μm particles in most defined separations!

The "ZORBAX RRHD-Wide Pore" is an LC column suitable for the analysis of biopharmaceuticals, providing highly reproducible separations of proteins and peptides. To efficiently separate proteins, peptides, or other macromolecules, the sample needs to be in close proximity to the stationary phase surface, which is why such a wide pore (300Å) column is necessary. Four different stationary phases (C18/C8/C3/Diphenyl) are available, allowing for the optimization of selectivity and recovery rates for proteins and polypeptides. 【Features】 ■ High-pressure (1200 bar) column for obtaining suitable results with the 1290 Infinity LC or other UHPLC systems ■ Usable with HILIC Plus ■ The selectivity remains the same for 3.5μm and 5μm ZORBAX columns with the same stationary phase, ensuring compatibility with various LCs *For more details, please refer to the PDF document or feel free to contact us.

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