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column Product List

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Fully porous HPLC column

You can easily change the method from a fully porous particle column to a surface porous particle column!

The product features various advantages of fully porous particle columns, such as high retention, load capacity, and solvent resistance. It can be expanded to suit your method, from UHPLC to preparative LC. Additionally, the InfinityLab Poroshell bonded phase is compatible with the traditional ZORBAX bonded phase, allowing for easy method changes from fully porous particle columns to superficially porous particle columns. 【How to choose the appropriate particle for your method (partial)】 ■1.8μm ZORBAX RRHD: Achieves the highest level of performance with UHPLC equipment - Maximum pressure: 1200 bar - Compatible systems: 1290 Infinity II LC or 1260 Infinity II Prime LC ■1.8 ZORBAX RRHT: Ultra-fast chromatography with a maximum of 600 bar - Maximum pressure: 600 bar - Compatible systems: 1260 Infinity II LC *For more details, please refer to the PDF document or feel free to contact us.

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ZORBAX Original Reverse Phase HPLC Column

It has a high activity level and helps in the separation of isomers and the improvement of selectivity!

We would like to introduce the "ZORBAX Original Reverse Phase HPLC Column" that we handle. Our lineup includes "ZORBAX C18," "ZORBAX C8," "ZORBAX-Phenyl," and others. These columns are manufactured using Type A silica and are useful for the separation of acidic or neutral compounds. Please feel free to consult us when you need assistance. 【ZORBAX C18 Specifications】 ■ZORBAX C18 - Pore Size: 70A - Surface Area: 300m²/g - Temperature Range: 60℃ - pH Range: 2.0-8.0 - End Cap: Yes - Carbon Content: 20% *For more details, please refer to the PDF document or feel free to contact us.

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Column "ZORBAX NH2"

ZORBAX offers both bonded and non-bonded series of stationary phases for reversed-phase HPLC columns!

"ZORBAX NH2" is a packing material that combines propylaminosilane with "ZORBAX SIL." It is used for normal phase separation, weak anion exchange, and reverse phase HPLC of polar compounds. When using this product, vitamins A and D are separated in normal phase mode, while hydrocarbons and sugars are separated in reverse phase mode. [Features] - Packing material that combines propylaminosilane with ZORBAX SIL - Used for normal phase separation, weak anion exchange, and reverse phase HPLC of polar compounds - Vitamins A/D are separated in normal phase mode, while hydrocarbons and sugars are separated in reverse phase mode *For more details, please refer to the related links or feel free to contact us.

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Column "Pursuit XRS/XRS Ultra"

The ultra-high purity base silica increases efficiency by 10-15% compared to a 3μm column!

The "Pursuit XRS/XRS Ultra" is a column that shortens analysis time while maintaining separation capability in general HPLC and LC/MS systems. The Agilent HPLC column series has been expanded with the addition of the "Pursuit XRs Ultra 2.8," an LC column with a particle size of 2.8μm. You can achieve rapid measurements while maintaining high separation efficiency with your current HPLC system. 【Features】 ■ Rapid analysis ■ High efficiency ■ High pressure resistance - Usable in general LC systems up to 1000 bar (=1020 kgf/cm2) *For more details, please refer to the related links or feel free to contact us.

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Column "AdvanceBio Peptide Mapping"

The particle size of 2.7μm allows for UHPLC performance in HPLC systems!

"AdvanceBio Peptide Mapping" is an LC column designed for biopharmaceutical analysis that provides high-resolution peptide maps for protein identification and post-translational modification measurement. Using this product, amino acid substitutions/modifications in the protein primary sequence can be rapidly separated and identified. This surface-porous advanced bio-column has a pore size of 120 Å, making it suitable for the analysis of peptides generated by enzymatic digestion of proteins. 【Features】 ■ Each batch is verified for stability and reproducibility using peptide standard substances. ■ Reliable analytical data can be obtained. ■ Identification of major peptides in complex peptide maps is possible. ■ UHPLC performance can be achieved with general HPLC equipment with a maximum pressure of 400 bar. ■ MS sensitivity is improved when using formic acid as the mobile phase in HPLC. *For more details, please refer to the PDF document or feel free to contact us.

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Column "ZORBAX Eclipse Amino Acid Analysis (AAA)"

The test for amino acid analysis (AAA) is complete! Using the general OPA and FMOC derivatization methods.

The "ZORBAX Eclipse Amino Acid Analysis (AAA)" is a column designed for high-speed separation of amino acids, following updated and improved protocols for use with Agilent HPLC systems. All analytical tasks between injections can be completed in 14 minutes (with a 10-minute analysis time) using a short 7.5 cm column, or in 24 minutes (with a 16-minute analysis time) using a long 15 cm column. By utilizing the OPA and FMOC derivatization methods in a fully automated single procedure with the Agilent HPLC system, very high sensitivity (5-50 pmol with DAD and FLD) and reliability are achieved. 【Features】 ■ High-resolution, high-speed analysis of 24 types of amino acids ■ Tested for amino acid analysis ■ Easily automated with detailed online derivatization protocols, compatible with Agilent HPLC autosamplers *For more details, please refer to the related links or feel free to contact us.

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ZORBAX Rx Reverse Phase HPLC Column

Rx-C18 is recommended for separation in low pH regions! It shows excellent stability and peak shape.

The "ZORBAX Rx Reverse Phase HPLC Column" is suitable for high-throughput analysis. It shows different selectivity compared to Eclipse XDB-C18 and StableBond SB-C18 in the low pH range, and StableBond is recommended for high-temperature applications. Additionally, Rx-C18 is manufactured using dimethyl octadecyl silane and demonstrates excellent stability up to pH 9 due to the lack of end-capping. 【Features】 ■ Shows different selectivity compared to Eclipse XDB-C18 and StableBond SB-C18 in the low pH range ■ Exhibits excellent stability and peak shape in low pH applications ■ Rx-C18 is manufactured using dimethyl octadecyl silane and demonstrates excellent stability up to pH 9 due to the lack of end-capping ■ Rx-C8 is the same product as SB-C8 *For more details, please refer to the PDF document or feel free to contact us.

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Column "Pursuit UPS"

Even in measurements requiring high speed and high separation, it can be performed using general HPLC or LC/MS!

"Pursuit UPS" is an LC column designed for high-speed analysis using your existing HPLC or LC/MS systems without the need for specialized equipment. By controlling the particle size to 2.4μm, it enables analysis that is 30-35% more efficient compared to columns with a particle size of 3μm. Additionally, it can reduce back pressure to about half compared to columns with a particle size of 2μm or less, allowing for rapid analysis with your already used LC systems. 【Features】 ■ Improved work efficiency - Compared to traditional 3μm particle size columns, it reduces peak retention time and peak width by 30-35%. *For more details, please refer to the related links or feel free to contact us.

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Capillary column/nano column

Compatible with various LC/MS interfaces! Available inner diameters of 0.5mm, 0.3mm, 0.1mm, and 0.075mm.

We would like to introduce our LC columns for bio-pharmaceutical analysis, 'Capillary Columns/Nano Columns.' You can choose from a wide variety of stationary phases, pore sizes, and dimensions. These columns are suitable for applications where the sample amount is extremely limited, as they enhance sensitivity by minimizing sample dilution within the column. 【Features】 ■ Extremely high sensitivity when the sample amount is very limited ■ Compatible with various LC/MS interfaces ■ Available inner diameters: 0.5mm, 0.3mm, 0.1mm, and 0.075mm ■ Stationary phases can accommodate a wide range of molecular weights, from small to large ■ Suitable for one-dimensional (1-D) and two-dimensional (2-D, proteomics) applications *For more details, please refer to the related links or feel free to contact us.

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ZORBAX RRHD Column

It shows the same selectivity as the 3.5 and 5µm ZORBAX columns using the same stationary phase!

The "ZORBAX Rapid Resolution High Definition (RRHD) column" is a product that further expands the lineup of ZORBAX columns with a particle size of 1.8µm. Through the optimization of the packing process, it achieves a pressure tolerance and stability of up to 120MPa (1200bar), suitable for use with the Agilent 1290 Infinity LC and other high-pressure HPLCs. Columns of lengths 50, 100, and 150mm are available, enabling the analysis of very complex samples at high speed or high separation. 【Features】 ■ Ultra-high pressure (120MPa [1200bar]) columns capable of achieving optimal results with the 1290 Infinity LC or other high-pressure HPLC systems ■ Achieves top-class separation ability with a particle size of 1.8µm ■ Two types of stationary phases are available: ZORBAX Eclipse Plus C18 for excellent peak shape, and ZORBAX StableBond C18 for superior selectivity and stability at low pH *For more details, please refer to the PDF document or feel free to contact us.

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Multiple Affinity Removal LC Column - Human 14

Removal of 14 types of excess protein using a simple method.

The "Agilent Human 14 Multiple Affinity Removal Column" is an LC column designed for biopharmaceutical analysis that removes 14 types of proteins, including abundant albumin, from human plasma samples, making it easier to separate and identify low-concentration proteins in human serum. By removing high-concentration proteins, it effectively expands the dynamic range of the analysis and enhances the performance of LC/MS and electrophoresis analysis of serum samples. It is compatible with most FPLC and HPLC pumps and instruments, including the Agilent 1200/1100 LC. 【Features】 ■ Removes approximately 94% of total protein ■ Available in three column sizes: - 4.6 x 50mm - 4.6 x 100mm - 10 x 100mm ■ Custom-made column sizes and spin cartridges are also available *For more details, please refer to the related links or feel free to contact us.

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Column "MicroBore"

When the sample mass is the same, the detection limit is improved by five times compared to a column with an inner diameter of 2.1 mm!

The "MicroBore" is a column suitable for the analysis of trace samples. This product uses low flow rates (generally below 50 μL/min). Therefore, it is suitable for use with detectors that require low flow rates, such as some mass spectrometers. The guard column can be adjusted for the depth of the tube stop, allowing for a complete zero dead volume connection at all times. 【Features】 ■ Suitable for use in HPLC systems purchased or modified for trace analysis ■ Achieves high sensitivity even with small sample volumes ■ Compatibility with LC/MS interfaces ■ A wide variety of stationary phases available ■ Guard columns that can be used with MicroBore columns (inner diameter 1.0 mm) and capillary columns *For more details, please refer to the related links or feel free to contact us.

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Column "Ultron ES"

Acetonitrile, ethanol, and phosphate buffer can be used as mobile phases!

"Ultron ES" is an insolubilized protein column equipped with asymmetric recognition sites for the optical isomer separation of numerous chiral compounds. It is designed to have a chiral stationary phase with two complementary protein bases, making it suitable for HPLC separation of optical isomers without derivatization. Additionally, it is suitable for the separation of pharmaceuticals. It can directly separate racemates without going through derivatization. 【Features】 ■ Ultron ES-OVM is used for the separation of optical isomers of acidic and basic pharmaceuticals. ■ Basic compounds that are difficult to separate with common products can sometimes be easily separated. ■ The ES-OVM column and ES-Pepsin column use a 120Å, 5μm silica packing that combines ovomucoid protein and pepsin protein. *For more details, please refer to the related links or feel free to contact us.

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Column "ZORBAX 300Extend-C18"

High robustness and separation of polypeptides and peptides over a wide range of pH from high pH to low pH (pH 2 to 11.5)!

The "ZORBAX 300Extend-C18" is a wide-bore HPLC column that efficiently separates peptides in the pH range of 2 to 11.5. Its unique bidentate bonding phase provides excellent longevity and reproducibility in both high and low pH regions. The retention characteristics and selectivity for peptides and polypeptides in the high pH region significantly change as these molecules become charged. 【Features】 ■ Shows different selectivity at high and low pH ■ Achieves excellent efficiency and good recovery rates for hydrophobic peptides at high pH ■ Suitable for LC/MS analysis using ammonium hydroxide mobile phase *For more details, please refer to the PDF document or feel free to contact us.

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Column "Hi-Plex"

High efficiency and low operating pressure! Separation of carbohydrates and alcohols is possible through ligand exchange.

"Hi-Plex" is a column that achieves high efficiency, low operating pressure, and long column life due to its monodisperse materials. With a series of ligand-to-ion pairs that provide suitable selectivity, resolution, and materials corresponding to the USP definitions for filler types "L17/19/34" and "L58," this product is ideal for isocratic separation using water or diluted acids as solutes. This simplifies HPLC system requirements and eliminates the need to use hazardous organic solvents. [Features] ■ Resolution and materials corresponding to the USP definitions for filler types L17/19/34 and L58 ■ Separation of carbohydrates and alcohols through ligand exchange ■ Separation of organic acids through ion exchange *For more details, please refer to the related links or feel free to contact us.

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